1. Functional Analysis of Sheep POU2F3 Isoforms
- Author
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Ning Wang, En-Guang Rong, Yan-Kai Chu, Hua Yang, Guangwei Ma, Xiaohong Yan, and Hui Li
- Subjects
Keratinocytes ,Male ,0301 basic medicine ,Gene isoform ,Keratin 14 ,Biology ,Biochemistry ,03 medical and health sciences ,0302 clinical medicine ,Promoter activity ,Matrix Metalloproteinases, Secreted ,Genetics ,medicine ,Animals ,Humans ,Protein Isoforms ,Molecular Biology ,Transcription factor ,Gene ,Cell proliferation ,Ecology, Evolution, Behavior and Systematics ,Sheep ,POU domain ,Cell growth ,Keratin-14 ,POU2F3 ,General Medicine ,Molecular biology ,HEK293 Cells ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Octamer Transcription Factors ,Original Article ,Isoforms ,Keratinocyte ,MMP19 - Abstract
POU domain class 2 transcription factor 3 (POU2F3) plays an important role in keratinocyte proliferation and differentiation. Our previous study identified four sheepPOU2F3transcript variants (POU2F3-1,POU2F3-2,POU2F3-3, andPOU2F3-4), encoding three POU2F3 protein isoforms (POU2F3-1, POU2F3-2, and POU2F3-3). However, the functional differences among the three POU2F3 isoforms remain unknown. The objective of this study was to determine the tissue expression pattern of the fourPOU2F3transcript variants in sheep and to investigate the functional differences in cell proliferation among the three POU2F3 isoforms. Quantitative RT-PCR analysis showed that the fourPOU2F3transcripts were ubiquitously expressed in all tested adult sheep tissues, andPOU2F3-1exhibited higher expression level than the other threePOU2F3transcript variants in skin (P P P P > 0.05). Dual luciferase reporter assays demonstrated that overexpression of any one of the three POU2F3 isoforms significantly inhibited the promoter activities of keratin 14 (KRT14) and matrix metalloproteinase 19 (MMP19) genes (P KRT14andMMP19genes than POU2F3-1 and POU2F3-2 (P P > 0.05). These results suggest three sheep POU2F3 isoforms have similar functional effects, but to a different extent.
- Published
- 2019