1. Adipocyte-derived extracellular vesicles regulate survival and function of pancreatic β cells
- Author
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Maria Felice Brizzi, Alessandro Fanciulli, Simone Arolfo, Maria Chiara Deregibus, Rita Nano, Gaia Zocaro, Iacopo Gesmundo, Huy Ong, Enrica Favaro, Stefania Bruno, Maria Alessandra Bocchiotti, Giovanni Birolo, Giacomo Gamba, Barbara Pardini, Giuseppe Matullo, Mario Morino, Justo P. Castaño, Giovanni Camussi, Eleonora Gargantini, Riccarda Granata, Dana Banfi, Ezio Ghigo, Raúl M. Luque, Lorenzo Piemonti, Juan M. Falcón-Pérez, Gabriele Togliatto, Noemi Congiusta, Gesmundo, Iacopo, Pardini, Barbara, Gargantini, Eleonora, Gamba, Giacomo, Birolo, Giovanni, Fanciulli, Alessandro, Banfi, Dana, Congiusta, Noemi, Favaro, Enrica, Deregibus, Maria Chiara, Togliatto, Gabriele, Zocaro, Gaia, Brizzi, Maria Felice, Luque, Raul M, Castaño, Justo P, Bocchiotti, Maria Alessandra, Arolfo, Simone, Bruno, Stefania, Nano, Rita, Morino, Mario, Piemonti, Lorenzo, Ong, Huy, Matullo, Giuseppe, Falcón-Pérez, Juan M, Ghigo, Ezio, Camussi, Giovanni, and Granata, Riccarda
- Subjects
0301 basic medicine ,Adult ,Male ,Programmed cell death ,Cell ,Adipose tissue ,Beta cells ,Diabetes ,Metabolism ,03 medical and health sciences ,chemistry.chemical_compound ,Extracellular Vesicles ,Islets of Langerhans ,Mice ,0302 clinical medicine ,Adipocyte ,3T3-L1 Cells ,microRNA ,medicine ,Adipocytes ,Animals ,Humans ,Obesity ,Chemistry ,Pancreatic islets ,General Medicine ,In vitro ,Cell biology ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Medicine ,Female ,Signal transduction ,Research Article - Abstract
Extracellular vesicles (EVs) are implicated in the crosstalk between adipocytes and other metabolic organs, and an altered biological cargo has been observed in EVs from human obese adipose tissue (AT). Yet, the role of adipocyte-derived EVs in pancreatic β cells remains to be determined. Here, we explored the effects of EVs released from adipocytes isolated from both rodents and humans and human AT explants on survival and function of pancreatic β cells and human pancreatic islets. EVs from healthy 3T3-L1 adipocytes increased survival and proliferation and promoted insulin secretion in INS-1E β cells and human pancreatic islets, both those untreated or exposed to cytokines or glucolipotoxicity, whereas EVs from inflamed adipocytes caused β cell death and dysfunction. Human lean adipocyte-derived EVs produced similar beneficial effects, whereas EVs from obese AT explants were harmful for human EndoC-βH3 β cells. We observed differential expression of miRNAs in EVs from healthy and inflamed adipocytes, as well as alteration in signaling pathways and expression of β cell genes, adipokines, and cytokines in recipient β cells. These in vitro results suggest that, depending on the physiopathological state of AT, adipocyte-derived EVs may influence β cell fate and function.
- Published
- 2020