1. Genetically Similar Isolates ofSalmonella entericaSerotype Enteritidis Persistent in China for a Long-Term Period
- Author
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Danyang Zhang, Xuanyi Shen, Hong Gao, Qifa Song, and Yuanbin Yang
- Subjects
0301 basic medicine ,Serotype ,Nalidixic acid ,Tetracycline ,Salmonella enteritidis ,030106 microbiology ,Biology ,Multiple Loci VNTR Analysis ,biology.organism_classification ,Virology ,Microbiology ,03 medical and health sciences ,Salmonella enterica ,Ampicillin ,medicine ,Pulsed-field gel electrophoresis ,Food Science ,medicine.drug - Abstract
Salmonella enterica serotype Enteritidis (S. Enteritidis) is an important causative agent of nontyphoidal salmonellosis in human populations. In this study, we collected 72 S. Enteritidis strains from 2004 to 2014 in Ningbo, mid-east China. Of the 72 strains, we identified a dominant clone of 58 strains recovered from patient's feces (n = 48), blood (n = 1), pleural effusion (n = 1), chickens (n = 3), and dessert cakes (n = 5) by pulsed-field gel electrophoresis (PFGE) and variable-number of tandem repeat analysis (MLVA). The profile arrangements of MLVA were SE1-SE2-SE3-SE5-SE6-SE8-SE9: 4-4-3-11-10-1-3. These dominant strains were susceptible to ampicillin, chloramphenicol, tetracycline, ciprofloxacin, gentamicin, cefotaxime and trimethoprim-sulfamethoxazole, and resistant to nalidixic acid. Additionally, all isolates harboured virulence genes invA, sipA, sopE, and spvB when tested by PCR. Our results reveal that genetically similar S. Enteritidis strains which accounted for several outbreaks as well as blood infection and pleural cavity infection are prevalent in China for a long-term period. This situation calls for further attention in the prevention and control of foodborne disease caused by Salmonella species.
- Published
- 2016