1. Occurrence and repair of alkylating stress in the intracellular pathogen Brucella abortus
- Author
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Xavier De Bolle, Arnaud Machelart, Katy Poncin, Eric Muraille, Emanuele G. Biondi, Nayla Francis, Agnès Roba, Kevin Willemart, Nicolas Zeippen, Georges Potemberg, Antonella Fioravanti, Stéphane P. Vincent, Ravikumar Jimmidi, Department of Bio-engineering Sciences, Research Unit in Molecular Biology (URBM-NARILIS), Université de Namur [Namur] (UNamur), Unité de Chimie Organique University of Namur, Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Université de Lille-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de chimie bactérienne (LCB), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institut de Microbiologie de la Méditerranée (IMM), University of Oxford [Oxford], Fédération Wallonie-Bruxelles (ARC 17/22-087) and by the FRS-FNRS 'Brucell-cycle' (PDR T.0060.15), ANR-11-JSV3-0003,CASTACC,Analyse comparative des facteurs de transduction des signaux contrôlant la régulation du cycle cellulaire chez les alpha-protéobactéries(2011), Université de Namur [Namur], Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), ANR: Analyse comparative des facteurs de transduction des signaux contrôlant la régulation du cycle cellulaire chez les alpha-protéobactéries – CASTACC,Projet CASTACC,ANR-JCJC-2011-Castacc, Université de Lille-Centre National de la Recherche Scientifique (CNRS), and University of Oxford
- Subjects
0301 basic medicine ,Alkylation ,DNA Repair ,[SDV]Life Sciences [q-bio] ,Cellular microbiology ,Brucella abortus ,General Physics and Astronomy ,medicine.disease_cause ,DNA damage response ,Mice ,chemistry.chemical_compound ,lcsh:Science ,Pathogen ,Multidisciplinary ,3. Good health ,Technologie de l'environnement, contrôle de la pollution ,Host-Pathogen Interactions ,Pathogens ,DNA repair ,Science ,030106 microbiology ,Biology ,Article ,Brucellosis ,General Biochemistry, Genetics and Molecular Biology ,Microbiology ,03 medical and health sciences ,Stress, Physiological ,medicine ,Animals ,Chimie ,Gene ,Escherichia coli ,Transcription factor ,Physique ,Macrophages ,DNA adducts ,Pathogenic bacteria ,General Chemistry ,DNA Methylation ,Astronomie ,RAW 264.7 Cells ,030104 developmental biology ,chemistry ,Vacuoles ,lcsh:Q ,DNA ,DNA Damage - Abstract
It is assumed that intracellular pathogenic bacteria have to cope with DNA alkylating stress within host cells. Here we use single-cell reporter systems to show that the pathogen Brucella abortus does encounter alkylating stress during the first hours of macrophage infection. Genes encoding direct repair and base-excision repair pathways are required by B. abortus to face this stress in vitro and in a mouse infection model. Among these genes, ogt is found to be under the control of the conserved cell-cycle transcription factor GcrA. Our results highlight that the control of DNA repair in B. abortus displays distinct features that are not present in model organisms such as Escherichia coli., SCOPUS: ar.j, info:eu-repo/semantics/published
- Published
- 2019