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Laine de Carvalho Guerra Pessoa Mamede,1 Rafaela Lira Formiga Cavalcanti de Lima,2 Alexandre Sérgio Silva,1 João Carlos Lima Rodrigues Pita,3 Nadjeanny Ingrid Galdino Gomes,1 Elisama Araújo de Sena,1 Rhayra Priscila Moraes Nobrega,2 João Otávio Scarano Alcântara,2 Julie Hanna Fontes de Souza,2 Glêbia Alexa Cardoso,3 José Luiz de Brito Alves,1 Maria da Conceição Rodrigues Gonçalves1 1Post-Graduate Program in Nutrition Sciences, Health Sciences Center, Federal University of Paraíba, João Pessoa, Brazil; 2Department of Nutrition, Health Sciences Center, Federal University of Paraíba, João Pessoa, Brazil; 3Health Sciences Center, Federal University of Paraíba, João Pessoa, BrazilCorrespondence: Laine de Carvalho Guerra Pessoa MamedeDepartment of Nutrition, Health Sciences Center, Universidade Federal da Paraíba, Campus I – Jd, Cidade Universitária, João Pessoa, PB, CEP: 58051-900, BrazilTel/Fax +55 83 9 91089818Email laineguerramamede@gmail.comAim: The study aimed to evaluate the effects of vitamin D3 (VD3) supplementation on inflammation and oxidative stress markers in overweight and obese women with deficiency or insufficiency of vitamin D.Methods: Twenty-nine overweight or obese women who had a deficiency or insufficiency of vitamin D were placed into two groups according to VD3 intervention. Patients in the supplemented group received a single oral megadose of VD3 (VD3, n=14). Patients in placebo group received a single oral identical capsule without vitamin D (placebo, n = 15). Anthropometric and biochemical variables were assessed at baseline and after 4-weeks intervention.Results: Anthropometric variables (waist circumference, waist–hip ratio, waist–height ratio and body mass index) were similar between groups (p > 0.05). VD3 supplementation increased the serum levels of 25-hydroxyvitamin D (p=0.000), malondialdehyde (p=0.021) and C-reactive protein (p=0.043) in overweight and obese women. Additionally, VD3 supplementation reduced the serum levels of aspartate aminotransferase (AST, p=0.035), alanine aminotransferase (ALT, p< 0.0001) in overweight and obese women. Despite this, the serum levels of parathyroid hormone (PTH), fasting glucose (FG), and alpha-1- acid glycoprotein (A1GPA), total antioxidant capacity (TAC) were similar between groups.Conclusion: In summary, a single oral megadose of VD3 increased 25-hydroxyvitamin D serum levels but did not improve oxidative stress and inflammation markers.Keywords: vitamin D, obesity, inflammation, lipid peroxidation

Objective: To analyze the hemodynamic variables and the rating of perceived exertion (RPE) between an active videogame (AVG) session and a dance session. Materials and Methods: The sample consisted...

This study aimed to verify the association between the genotypic of the receptor gene activated by peroxisome proliferators gamma 2 (PPARy2) and the body composition and the specific indicators of adiposity in practitioners physical exercises, considering nutritional intake, age, and training load as influencing factors. It was conducted a cross-sectional study with 335 adults (47.9 ± 12.7 years, 138 men, body mass index/BMI = 27.0 ± 4.9 kg/m2), practitioners of aerobic exercises in cyclical modalities (running, walking and/or cycling, who spent 328.3 ± 193.6 kcal/day on physical training). The genotyping of the Pro12Ala polymorphism was performed using the PCR-RFLP technique and the body composition measured by bioimpedance (InBody 720). Energy expenditure was based on the compendium of physical activities and caloric intake was measured by 24 h recall questionnaire. The higher prevalence was for the Pro/Pro genotype (76.1% vs. 23.9% of Pro/Ala). Pro/Pro genotypic group showed significant higher mean values for body mass (BM) (p < 0.03 for men and p < 0.02 for women) and BMI (p < 0.00 for men and p < 0.02 for women) and %FAT (p < 0.00), waist-hip ratio (WHR) (p < 0.04), and visceral fat (VF) (p < 0.00) only in men compared to Pro/Ala. Higher frequency of Pro/Pro was observed in the category indicating BMI (p < 0.00 for men and p < 0.03 for women), WRH (p < 0.03 for men and p < 0.00 for women), and %FAT (p < 0.03) (in the latter case, only among men. It was also observed that the frequency of distribution of Pro/Ala in the eutrophic category of the BMI remained independent of all influencers, while WHR and %FAT were independent of the training load, but influenced by nutritional intake and age. In women, the frequency of Pro/Ala distribution at the lowest BMI and WHR values remained independent of all confounding variables. It is concluded that the Pro12Ala polymorphism in the PPARy2 gene consistently influences indicators of body composition and adiposity, regardless of the practitioners of physical training, but the relationship needs to be considered according to age and nutritional intake.

This study analyzed the intensity of Kinect Sports® games in college students through hemodynamic variables and perceived exertion. An experimental study, with 34 young students (21.5 ± 1.9 years) who underwent three consecutive games sessions, divided into boxing, bowling, baseball, and tennis games; sand, golf, athletics, and ski flights; table tennis, football, darts, and American football. Heart rate (HR), blood pressure (BP), and rate of perceived exertion (RPE) were recorded every two minutes of each game. One-way ANOVA for repeated measurements was used to verify differences in HR, BP, and RPE at each time of the game and the effect size (p, RESUMO Este estudo analisou a intensidade dos jogos Kinect Sports® em universitários através das variáveis hemodinâmicas e da percepção subjetiva de esforço. Tratou-se de um estudo experimental, com 34 jovens estudantes (21.5±1.9anos) que foram submetidos a três sessões consecutivas de jogos, divididas em jogos de boxe, boliche, beisebol e tênis; vôlei de areia, golfe, atletismo e esqui; tênis de mesa, futebol, dardos e futebol americano. A frequência cardíaca (FC), pressão arterial (PA) e percepção subjetiva de esforço (PSE) foram registradas a cada dois minutos de cada jogo. One-way ANOVA for repeated measurements was used to verify differences in HR, BP and PSE at each time of the game and the effect size (p

The objective of this study was to verify the influence of the Pro12Ala polymorphism of the PPARγ2 gene in response of a training program on the body composition. Sixty-nine previously inactive men and women (32.8 ± 8.2 years) were genotyped and underwent a 12-week aerobic (running/walking) training program (3–5 sessions, 40 – 60 min per session, and intensity between the aerobic and anaerobic threshold) (experimental group n = 53) or were part of the control group (n = 16). They were tested for aerobic capacity (ergospirometry), body composition (DXA), abdomen, waist and hip circumferences and nutritional assessment before and 48 h after the experimental protocol. Two-way repeated measures ANOVA test was used to verify possible differences in variables between the experimental vs. control groups or Pro/Pro vs. Pro/Ala groups, and the Chi-squared test was used to verify the distribution of responders and non-responders according to genotype (p < 0.05). Frequencies of 75.5% Pro/Pro (n = 40) and 24.5% Pro/Ala (n = 13) were found, without any occurrence of the recessive homozygote. Body fat reduction was initially confirmed compared to a control group which did not exercise (n = 16; 29.1 ± 8.8 years), so that the exercise group obtained a reduction of −1.3 kg vs. −0.3 kg in the control group (p = 0.03). When they were divided by genotype, there were significant changes in fat mass (−1.3 ± 2.1 kg; p = 0.00), lean mass (0.6 ± 1.5 kg; p = 0.02), fat percentage (−1.3 ± 1.6; p = 0.00), waist circumference (−2.2 ± 2.9 cm; p = 0.00), abdomen circumference (−3.3 ± 3.6 cm; p = 0.00) and hip circumference (−2.7 ± 2.7 cm; p = 0.00) for Pro/Pro genotypes; and fat mass (−1.1 ± 1.7 kg; p = 0.04), fat percentage (−0.9 ± 1.5; p = 0.04), abdomen circumference (−3.9 ± 3.5 cm; p = 0.00) and hip circumference (−1.8 ± 1.8 cm; p = 0.00) for Pro/Ala genotypes, without any group interaction differences. The Chi squared test revealed no differences in the distribution of responders or non-responders according to genotype. It is concluded that an aerobic training program promotes weight loss, but the Pro12Ala polymorphism in the PPARγ2 gene does not influence the variability of aerobic-induced exercise weight loss.

Background High levels of oxidative stress promote degradation of the cell membrane impairing cellular function in fat oxidation. However, the influence of oxidative stress on exercise-induced weight-loss has not yet been investigated. Therefore, the aim of this study was to verify the influence of a lipidic peroxidation marker (malondialdehyde, MDA) and antioxidant status (total antioxidant capacity marker, TAC) on the magnitude of weight-loss by aerobic-induced exercise in previously sedentary overweight or obese individuals. Methods Seventy-five physically inactive adults were randomized into experimental (N.=58) and control (N.=17) groups, who engaged in a 12-week program of aerobic training walking and/or running (3 to 5 days/week) or stretching (1 day/week), respectively. Body composition (DXA), aerobic capacity (ergospirometric) and blood collections for oxidative stress analysis (MDA and TAC) were determined before and after the experimental protocol. Two-way ANOVA for repeated measures or Friedman's test were used to evaluate differences in time/group interaction. Pearson correlation was used to verify the relationship between the variables of oxidative stress and of body composition. Results Significant reduction was found in fat body mass of experimental when compared to control group (-1.3±1.9 kg versus -0.3±1.3, P=0.04). Experimental group also altered significantly the total body mass (-1.2±4.7 kg; effect size 0.44), body mass index - BMI (-0.3±1.1 effect size 0.37), fat percentage (1.3±1.6%; effect size 0.50) and lean body mass (0.6±1.5 kg; effect size 0.32).There was increase in MDA of 2.3 μmol/L to 2.7 μmol/L (P=0.00), without changes to TAC (25.6±13.9% to 28.0±10.4%). No correlation was found between these variations in body composition with either the initial values of MDA and TAC or delta variation of these indicators of oxidative stress in response to the training program. Conclusions Indicators of oxidative stress (MDA and TAC) does not influence the magnitude of weight-loss induced by aerobic training.

Asthma is a heterogeneous disease of the airways characterized by chronic inflammation associated with bronchial and smooth muscle hyperresponsiveness. Currently, different murine models for the study of asthma show poor bronchial hyperresponsiveness due to a scarcity of smooth muscle and large airways, resulting in a failure to reproduce smooth muscle hyperreactivity. Thus, we aimed to standardize a guinea pig model of chronic allergic lung inflammation mimicking airway smooth muscle hyperreactivity observed in asthmatics (Asth). Animals were randomly divided into a control group (Ctrl), which received saline (0.9% NaCl), and the Asth group, subjected to in vivo sensitization with ovalbumin (OVA) nebulization. Morphological analysis was performed by hematoxylin-eosin staining. Bronchial hyperresponsiveness was evaluated by nebulization time in the fifth, sixth, and seventh inhalations (NT5-7) and tracheal isometric contractions were assessed by force transducer. Total antioxidant capacity was measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and protein expression by Western blot. Histologically, the Asth group developed peribronchial cellular infiltrate, epithelial hyperplasia and smooth muscle thickening. After the fourth nebulization, the Asth group developed bronchial hyperreactivity. The trachea from the Asth group contracted after in vitro stimulation with OVA, differing from the Ctrl group, which showed no response. Additionally, airway smooth muscle hyperreactivity to carbachol and histamine was observed in the Asth group only in intact epithelium preparations, but not to KCl, and this effect was associated with an augmented production of reactive oxygen species. Moreover, lung inflammation impaired the relaxant potency of isoproterenol only in intact epithelium preparations, without interfering with nifedipine, and it was found to be produced by transforming growth factor-β negative modulation of β adrenergic receptors and, furthermore, big-conductance Ca2+-sensitive K+ channels. These effects were also associated with increased levels of phosphatidylinositol 3-kinases but not extracellular signal-regulated kinases 1/2 or phosphorylation, and augmented α-actin content as well, explaining the increased smooth muscle mass. Furthermore, pulmonary antioxidant capacity was impaired in the Asth group. Therefore, we developed a standardized and easy-to-use, reproducible guinea pig model of lung inflammation that mimics airway smooth muscle hypercontractility, facilitating the investigation of the mechanisms of bronchial hyperresponsiveness in asthma and new therapeutic alternatives.

AIMS to compare the influence of menopause on body fat, induced by a physical training program. METHODS Twenty-one sedentary women, 10 postmenopausal (MN; 54.6 ± 5.1 years, 42.9 ± 4.1 % body fat) and 11 in the ovulatory state (OS; 35.1 ± 5.4 years, 44.1 ± 5.5 % body fat), performed a training program with aerobic exercise (12 weeks, five times / week, 60 minutes per session). Before and after this program, the following measurements were taken: sum of skin fold (∑D), body fat percentage (%F), waist-hip ratio (WHR) and abdominal circumference (AC). RESULTS MN and OS significantly reduced ΣD (38.5 ± 25.1 mm and 27.7 ± 19.4 mm, respectively), but without differences between the groups. MN group women obtained relative reduction in the suprailiac fold, significantly greater than OS (25 ± 10 vs. 11.5 ± 12 mm), and also a greater reduction in the %F (13.5 ± 8% vs. 6.6 ± 6%). There were very slight reductions in WHR in both groups (0.009 ± 0.02 and 0.005 ± 0.03 in MN and OS, respectively), as well as AC (reduction of 3.5 ± 3 cm and 3.9 ± 2.9 cm for MN and OS), with no differences between groups. CONCLUSION The postmenopausal women responded to a training program with a greater reduction of body fat, but in only one of four evaluated body composition variables.

Highlights • Post-exercise hypotension did not occur in anabolic steroid users. • The rehabilitation of hypertension is blunted by anabolic steroids. • Damage caused by anabolic steroids should be reported in educational programs., Background There is evidence of hypertensive effects caused by anabolic androgenic steroids (AAS). A single exercise session promotes the acute reduction of blood pressure, but the effects of AAS on this phenomenon are unknown. Objectives To investigate the post-exercise blood pressure response in androgenic-anabolic steroid users. Methods Thirteen AAS users (23.9 ± 4.3 years old) and sixteen controls (22.1 ± 4.5 years old) performed a session of aerobic exercise. Heart rate and blood pressure were assessed before exercise and during a 60 min post-exercise resting period. Repeated ANOVA measures were used to determine differences between the groups. Results While the control group had a significant reduction in post-exercise systolic blood pressure of up to 13.9 ± 11.6 mmHg at 40 min, this phenomenon was limited among AAS users who reached a maximum of 6.2 ± 11.5 mmHg at 60 min. The between groups comparison revealed significant higher post-exercise hypotension (PEH) for the control group at 30 min (−12.9 ± 14.1 mmHg versus −2.9 ± 7.6 mmHg), 40 min (−13.9 ± 11.6 mmHg versus −2.5 ± 8.3 mmHg), 50 min (−13.9 ± 13.9 mmHg versus −5.0 ± 7.9 mmHg) and 60 min (−12.5 ± 12.8 mmHg versus −6.2 ± 11.5 mmHg). There was no significant diastolic PEH in any of the groups. Conclusions This study demonstrated impaired systolic post-exercise hypotension as a new adverse effect of AAS usage.