1. Detection of Toxoplasma gondii in Crassostrea spp. oysters cultured in an estuarine region in eastern Amazon
- Author
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Jacqueline Silva, Alessandra Scofield, Flávia de Nazaré Leite Barros, Gleiciane Schupp de Sena Mesquita, Marcely Karen Santos do Rosário, Maeli Fernanda Silva Ferreira, Betsy Emely Tavares Honorio, Thamillys Rayssa Marques Monteiro, Isis Abel, Carla Cristina Guimarães de Moraes, Katarine de Souza Rocha, and Hugo Filipe Rodrigues Melo
- Subjects
0301 basic medicine ,Gill ,animal structures ,Epidemiology ,030231 tropical medicine ,030106 microbiology ,DNA sequencing ,Host-Parasite Interactions ,Microbiology ,03 medical and health sciences ,0302 clinical medicine ,parasitic diseases ,Animals ,Crassostrea ,Gene ,General Veterinary ,General Immunology and Microbiology ,biology ,fungi ,Public Health, Environmental and Occupational Health ,Toxoplasma gondii ,biology.organism_classification ,DNA extraction ,Infectious Diseases ,GenBank ,Estuaries ,Toxoplasma ,Nested polymerase chain reaction ,Brazil - Abstract
The aim of the present study was to detect DNA of Toxoplasma gondii in Crassostrea spp. oysters cultured in the state of Pará, Brazil. A total of 400 oysters were directly collected from a fixed rack system. Gills, gastrointestinal tract (GIT) and intervalvular liquid were separated and grouped into pool samples of 10 animals, resulting in 40 samples each of gills, GIT and intervalvular liquid. DNA extraction was performed using a commercial kit, and T. gondii DNA was detected by nested PCR using the primers Toxo3 and Toxo4, which produced an amplification product of 155 bp of the T. gondii gene B1. Nucleotide sequencing was performed for positive samples, and the obtained sequences were identified by comparison with sequences in GenBank. The DNA of T. gondii was detected in 5.8% (7/120) of the pool samples, of which 7.5% (3/40) was in the GIT, 5% (2/40) in the gills, and 5% (2/40) was in the intervalvular liquid. The obtained sequences presented 100% identity and overlap with T. gondii DNA sequences. This is the report of detection of T. gondii DNA in oysters from genus Crassostrea spp. originating from the state of Pará, eastern Amazon.
- Published
- 2019
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