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Start Over Descriptor "MESH : Intestinal Mucosa" Topic 0302 clinical medicine
4 results on '"MESH : Intestinal Mucosa"'

1. Expression colique de l’interféron-γ et de l’interleukine-10 au cours de la maladie de Crohn et de la rectocolite ulcéro-hémorragique

Author

A. Sassi, Lamia Kallel, M. Ben Ahmed, Samir Boubaker, Hechmi Louzir, Béchir Zouari, Azza Filali, Jalel Boubaker, Service de gastroentérologie A, Hôpital de La Rabta Tunis, Laboratoire de Transmission, Contrôle et Immunobiologie des Infections ( LR11IPT02 ), Institut Pasteur de Tunis-Réseau International des Instituts Pasteur ( RIIP ), Institut Pasteur de Tunis, Département de médecine préventive et de statistiques, Faculté de Médecine de Tunis, Hôpital La Rabta [Tunis], Laboratoire de Transmission, Contrôle et Immunobiologie des Infections - Laboratory of Transmission, Control and Immunobiology of Infection (LR11IPT02), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), and Réseau International des Instituts Pasteur (RIIP)

Subjects
MESH: Interleukin-10, MESH : RNA, Messenger, [SDV]Life Sciences [q-bio], MESH: Down-Regulation, MESH : Down-Regulation, MESH: Biopsy, 0302 clinical medicine, MESH : Child, MESH: Reverse Transcriptase Polymerase Chain Reaction, MESH: Child, Gamma interferon, Medicine, MESH : Female, MESH : Colonoscopy, 0303 health sciences, MESH: Middle Aged, MESH : Immunity, Mucosal, MESH : RNA, Ribosomal, MESH : Reverse Transcriptase Polymerase Chain Reaction, General Medicine, MESH : Adult, MESH : Colitis, Ulcerative, MESH: Case-Control Studies, MESH: Colonoscopy, MESH: Intestinal Mucosa, MESH : Severity of Illness Index, [ SDV.MHEP.HEG ] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, 030211 gastroenterology & hepatology, MESH : Case-Control Studies, MESH: Gene Expression, MESH: Interferon-gamma, MESH : Male, MESH: Colitis, Ulcerative, MESH : Crohn Disease, MESH : Intestinal Mucosa, MESH: Gene Expression Profiling, 03 medical and health sciences, MESH: Severity of Illness Index, MESH : Adolescent, MESH : Interleukin-10, MESH : Middle Aged, MESH: Colon, MESH : Interferon-gamma, MESH: RNA, Messenger, 030304 developmental biology, MESH: Adolescent, MESH: Humans, MESH: Crohn Disease, [ SDV ] Life Sciences [q-bio], Crohn disease, business.industry, MESH: Transcription, Genetic, MESH : Gene Expression Profiling, MESH : Humans, MESH : Transcription, Genetic, MESH: Adult, [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, MESH : Colon, Molecular biology, MESH: Male, MESH : Gene Expression, MESH: Immunity, Mucosal, MESH : Biopsy, MESH: RNA, Ribosomal, business, MESH: Female
Abstract

Resume Objectif Le but de ce travail etait d’explorer l’expression colique de l’INF-γ et de l’IL-10 chez des patients atteints de maladie de Crohn (MC) ou de rectocolite ulcero-hemorragique (RCH). Methodes Quatorze patients atteints de MC et 11 atteints de RCH ont ete inclus, ainsi que 7 sujets temoins. L’etude de l’expression colique de l’INF-γ et de l’IL-10 a ete realisee sur des biopsies de colon sain et malade, en utilisant la technique de transcription inverse et d’amplification genique (RT-PCR) en temps reel (Taqman). Les resultats ont ete exprimes sous forme de rapport entre la quantite d’ARN messager (ARNm) de la cytokine et celle d’une molecule de reference, l’ARN ribosomal 18S, puis multiplie par 10 8 . Resultats En cas de maladie de Crohn, le colon exprimait plus d’INF-γ et d’IL-10 par rapport aux temoins (medianes respectives : 23,03 vs 1,87, p = 0,042 et 20,61 vs 2,13, p = 0,08). Une puissante correlation positive a ete trouvee entre l’expression colique de l’IL-10 et celle de l’INF-γ au cours de la MC (r = 0,9, p Au niveau des sites lesionnels de RCH, l’expression de l’INF-γ ainsi que celle de l’IL-10 etaient comparables a celles observees chez les temoins (7,18 vs 2,18, p = 0,36 et 3,66 vs 1,87, p = 0,44). Conclusion Au cours de la maladie de Crohn, l’expression de l’IL-10 et celle de l’INF-γ etaient plus elevees que chez les temoins et etroitement correlees.

Published
2005
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2. Confocal laser endomicroscopy is a new imaging modality for recognition of intramucosal bacteria in inflammatory bowel disease in vivo

Author

Marcus Kerner, Bernard Flourié, Stephan Biesterfeld, Peter R. Galle, Martin Goetz, Barry J. Campbell, Jean-Christophe Saurin, Driffa Moussata, Alastair J.M. Watson, Annabel Gloeckner, Arthur Hoffman, Ralf Kiesslich, Markus F. Neurath, Hepato-Gastroenterology, Centre Hospitalier Lyon Sud [CHU - HCL] ( CHLS ), Hospices Civils de Lyon ( HCL ) -Hospices Civils de Lyon ( HCL ), university of mainz, Johannes Gutenberg - University of Mainz ( JGU ), Univeristy of mainz, University of Liverpool, Equipe 8, Centre de Recherche en Cancérologie de Lyon ( CRCL ), Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Centre Léon Bérard [Lyon]-Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Centre Léon Bérard [Lyon]-Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -Hôpital Edouard Herriot [CHU - HCL], Centre Hospitalier Lyon Sud [CHU - HCL] (CHLS), Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), Johannes Gutenberg - Universität Mainz (JGU), Centre de Recherche en Cancérologie de Lyon (UNICANCER/CRCL), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hôpital Edouard Herriot [CHU - HCL]

Subjects
Pathology, MESH : Escherichia coli, fluorescein, MESH : Retrospective Studies, Colorectal cancer, MESH : Prospective Studies, Gastroenterology, Inflammatory bowel disease, [ SDV.CAN ] Life Sciences [q-bio]/Cancer, 0302 clinical medicine, Intestinal mucosa, MESH: Microscopy, Confocal, MESH: Animals, MESH : Colonoscopy, MESH: In Situ Hybridization, Fluorescence, intramucosal bacteria, 0303 health sciences, Crohn's disease, MESH: Escherichia coli, MESH : Enterobacteriaceae, MESH : Colitis, Ulcerative, Ulcerative colitis, enteric bacterial microflora, 3. Good health, MESH : In Situ Hybridization, Fluorescence, MESH: Colonoscopy, confocal laser endomicroscopy, MESH: Intestinal Mucosa, MESH : Inflammatory Bowel Diseases, 030211 gastroenterology & hepatology, medicine.medical_specialty, MESH : Male, MESH: Colitis, Ulcerative, [SDV.CAN]Life Sciences [q-bio]/Cancer, MESH : Mice, Inbred C57BL, Biology, MESH : Intestinal Mucosa, MESH : Crohn Disease, 03 medical and health sciences, MESH: Enterobacteriaceae, FISH, fluorescence endoscopy, In vivo, MESH: Mice, Inbred C57BL, Internal medicine, MESH : Mice, medicine, Endomicroscopy, MESH: Colon, MESH : Microscopy, Confocal, MESH: Mice, ulcerative colitis, 030304 developmental biology, MESH : Ileum, MESH: Humans, Bacteria, MESH: Crohn Disease, infectious colitis, MESH : Humans, Endoscopy, MESH: Retrospective Studies, medicine.disease, MESH: Inflammatory Bowel Diseases, MESH : Colon, MESH: Male, MESH: Prospective Studies, MESH: Ileum, MESH : Animals, Ex vivo
Abstract

International audience; BACKGROUND AND OBJECTIVES: Interaction of bacteria with the immune system within the intestinal mucosa plays a key role in the pathogenesis of inflammatory bowel disease (IBD). The aim of the current study was to develop a fluorescein-aided confocal laser endomicroscopy (CLE) method to visualise intramucosal enteric bacteria in vivo and to determine the involved mucosal area in the colon and ileum in patients with ulcerative colitis (UC) and Crohn's disease (CD). METHODS: Initially, E coli strains expressing enhanced green fluorescent protein (pEGFP) were endomicroscopically imaged in mice. In addition, ex vivo and in vivo imaging of fluorescent human enteric bacteria was performed to specify the distinct endomicroscopic appearance of enteral bacteria. Targeted mucosal biopsies towards endomicroscopic identifiable intramucosal bacteria and negative mucosal areas were prospectively obtained during colonoscopy and correlated with bench-top fluorescence microscopy (FISH) to prove the endomicroscopic visualisation of intramucosal bacteria. Finally, a retrospective analysis as well as a prospective study was performed in patients with UC and CD to confirm the presence and distribution of intramucosal bacteria within the gut. RESULTS: Confocal endomicroscopy was able to identify intramucosal pEGFP E coli in mice and strains of enteric microflora in the mucosa of humans. Using FISH as the gold standard, evaluation of 21 patients showed that CLE had a sensitivity of 89% and specificity of 100% to identify intramucosal bacteria. In a retrospective study, 113 patients with CD and UC had intramucosal bacteria significantly more often than 50 control patients (66% vs 60% vs 14%, p

Published
2011
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3. Bacterial Toxins and the Nervous System: Neurotoxins and Multipotential Toxins Interacting with Neuronal Cells

Author

Bernard Poulain, Michel R. Popoff, Bactéries anaérobies et Toxines, Institut Pasteur [Paris] (IP), Neurotransmission et sécrétion neuroendocrine (NSN), Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris], Neurotransmission et sécrétion neuroendocrine ( NSN ), and Centre National de la Recherche Scientifique ( CNRS )

Subjects
Nervous system, rho GTP-Binding Proteins, actin cytoskeleton, Health, Toxicology and Mutagenesis, [SDV]Life Sciences [q-bio], [ SDV.TOX ] Life Sciences [q-bio]/Toxicology, MESH: Neurons, MESH : Actins, Apoptosis, Review, Toxicology, Synaptic Transmission, 0302 clinical medicine, Intestinal mucosa, MESH : Glutamic Acid, Neurotoxin, MESH: Animals, Intestinal Mucosa, toxin, Neurons, 0303 health sciences, MESH : Synaptic Transmission, MESH: Exocytosis, nervous system, MESH: Glutamic Acid, 3. Good health, Cell biology, medicine.anatomical_structure, MESH: Calcium, [SDV.TOX]Life Sciences [q-bio]/Toxicology, MESH : Monomeric GTP-Binding Proteins, Enterochromaffin cell, MESH: Intestinal Mucosa, SNARE Proteins, neurotransmitter, MESH: SNARE Proteins, Cell type, Bacterial Toxins, Neurotoxins, Glutamic Acid, MESH: Monomeric GTP-Binding Proteins, Biology, Blood–brain barrier, MESH: Actins, MESH : Neurotoxins, MESH : Intestinal Mucosa, MESH : Neurons, MESH : Exocytosis, Exocytosis, small gtpases, 03 medical and health sciences, MESH : rho GTP-Binding Proteins, medicine, MESH: Synaptic Transmission, Animals, Humans, MESH : Calcium, 030304 developmental biology, Monomeric GTP-Binding Proteins, MESH: Neurotoxins, MESH: Humans, [ SDV ] Life Sciences [q-bio], MESH: Apoptosis, MESH : Humans, neurotoxin, Actin cytoskeleton, MESH : SNARE Proteins, MESH: rho GTP-Binding Proteins, Actins, MESH: Bacterial Toxins, MESH : Bacterial Toxins, Enteric nervous system, Calcium, MESH : Animals, enterotoxin, 030217 neurology & neurosurgery, MESH : Apoptosis
Abstract

International audience; Toxins are potent molecules used by various bacteria to interact with a host organism. Some of them specifically act on neuronal cells (clostridial neurotoxins) leading to characteristics neurological affections. But many other toxins are multifunctional and recognize a wider range of cell types including neuronal cells. Various enterotoxins interact with the enteric nervous system, for example by stimulating afferent neurons or inducing neurotransmitter release from enterochromaffin cells which result either in vomiting, in amplification of the diarrhea, or in intestinal inflammation process. Other toxins can pass the blood brain barrier and directly act on specific neurons.

Published
2010
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4. Sumoylation by Ubc9 Regulates the Stem Cell Compartment and Structure and Function of the Intestinal Epithelium in Mice

Author

Esther Danenberg, Alexandra Andrieux, Karim Nacerddine, Ana Cumano, Ghislaine Hamard, Perrine Bomme, Grégory Jouvion, Anne Dejean, Béatrice Romagnolo, Maud D. Demarque, Nick Barker, Hélène Neyret–Kahn, Hans Clevers, Benoit Terris, Hubrecht Institute for Developmental Biology and Stem Cell Research, Organisation Nucléaire et Oncogenèse / Nuclear Organization and Oncogenesis, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Histotechnologie et Pathologie, Institut Pasteur [Paris], Microscopie Ultrastructurale (Plate-forme), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Plateforme Recombinaison Homologue, Transfert d'Embryons et Cryoconservation [Institut Cochin] (PRHTEC), Institut Cochin (IC UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut Cochin (UMR_S567 / UMR 8104), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5), Service d'Anatomie Pathologique, Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Développement des Lymphocytes, Organisation Nucléaire et Oncogenèse, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS ), Plateforme Recombinaison Homologue, Transfert d'Embryons et Cryoconservation [Institut Cochin] ( PRHTEC ), Institut Cochin ( UM3 (UMR 8104 / U1016) ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Institut Cochin ( UMR_S567 / UMR 8104 ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), CHU Cochin [AP-HP], Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), and Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
SUMO protein, MESH : Tamoxifen, MESH: Mice, Knockout, Mice, 0302 clinical medicine, Keratin, [ SDV.IMM ] Life Sciences [q-bio]/Immunology, MESH: Animals, Intestinal Mucosa, health care economics and organizations, chemistry.chemical_classification, Mice, Knockout, 0303 health sciences, Stem Cells, MESH: Sumoylation, Gastroenterology, LGR5, MESH : Sumoylation, Intestinal epithelium, Cell biology, MESH : Phenotype, medicine.anatomical_structure, Phenotype, Biochemistry, MESH : Stem Cells, MESH: Intestinal Mucosa, [SDV.IMM]Life Sciences [q-bio]/Immunology, Basal lamina, Stem cell, MESH : Ubiquitin-Conjugating Enzymes, education, MESH: Stem Cells, Biology, MESH: Phenotype, MESH : Intestinal Mucosa, 03 medical and health sciences, MESH : Mice, medicine, Animals, MESH: Mice, 030304 developmental biology, Hepatology, Sumoylation, Small intestine, MESH: Ubiquitin-Conjugating Enzymes, Tamoxifen, chemistry, Ubiquitin-Conjugating Enzymes, Keratin 8, MESH : Mice, Knockout, MESH: Tamoxifen, MESH : Animals, 030217 neurology & neurosurgery
Abstract

Background & Aims Small ubiquitin-like modifiers (SUMOs) are attached to other proteins to regulate their function (sumoylation). We investigated the role of Ubc9, which covalently attaches SUMOs to proteins, in the gastrointestinal tract of adult mice. Methods We investigated the effects of decreased sumoylation in adult mammals by generating mice with an inducible knockout (by injection of 4-hydroxytamoxifen) of the E2 enzyme Ubc9 ( Ubc9fl/-/ROSA26-CreERT2 mice). We analyzed the phenotypes using a range of histologic techniques. Results Loss of Ubc9 from adult mice primarily affected the small intestine. Ubc9fl/−/ROSA26-CreERT2 mice died within 6 days of 4-hydroxytamoxifen injection, losing 20% or less of their body weight and developing severe diarrhea on the second day after injection. Surprisingly, other epithelial tissues appeared to be unaffected at that stage. Decreased sumoylation led to the depletion of the intestinal proliferative compartment and to the rapid disappearance of stem cells. Sumoylation was required to separate the proliferative and differentiated compartments from the crypt and control differentiation and function of the secretory lineage. Sumoylation was required for nucleus positioning and polarized organization of actin in the enterocytes. Loss of sumoylation caused detachment of the enterocytes from the basal lamina, as observed in tissue fragility diseases. We identified the intermediate filament keratin 8 as a SUMO substrate in epithelial cells. Conclusions Sumoylation maintains intestinal stem cells and the architecture, mechanical stability, and function of the intestinal epithelium of mice.

Published
2010
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