1. Novel in vitro and in vivo anti-Helicobacter pylori effects of pomegranate peel ethanol extract
- Author
-
Amal Mayyas, Mohammad Abu-Sini, Rula Amr, Rand T. Akasheh, Waleed Zalloum, Ayman Khdair, Islam Hamad, Talal Aburjai, Rula M. Darwish, and Luay Abu-Qatouseh
- Subjects
medicine.drug_class ,Veterinary medicine ,Antibiotics ,Pharmacology ,SF1-1100 ,03 medical and health sciences ,Minimum inhibitory concentration ,0302 clinical medicine ,In vivo ,SF600-1100 ,medicine ,urease inhibition ,IC50 ,030304 developmental biology ,0303 health sciences ,General Veterinary ,biology ,Chemistry ,in vitro ,Helicobacter pylori ,Antimicrobial ,biology.organism_classification ,pomegranate peel ethanol extracts ,Animal culture ,in vivo ,Metronidazole ,Punica ,helicobacter pylori ,030211 gastroenterology & hepatology ,medicine.drug - Abstract
Background and Aim: Interest in plants with antimicrobial properties has been revived due to emerging problems associated with using antibiotics to eradicate Helicobacter pylori. Accordingly, this study aims to assess the antibacterial effects of Punica granatum and the possible synergistic effect of its extract along with metronidazole against H. pylori. Materials and Methods: Pomegranate peel ethanol extracts (PPEE) was tested against a control strain of H. pylori (NCTC 11916) in vitro and in vivo in female Wistar rats. Moreover, the synergistic effect of PPEE in combination with metronidazole was tested in vitro. Results: The PPEE exhibited a remarkable activity against H. pylori with a minimum inhibitory concentration (MIC) of 0.156 mg/mL. Furthermore, the extract exhibited a pronounced urease inhibitory activity (IC50 ∼6 mg/mL) against the tested strain. A synergistic effect between PPEE and metronidazole was also observed (fractional inhibitory concentrations
- Published
- 2021
- Full Text
- View/download PDF