1. Distinct patterns of somatic alterations in a lymphoblastoid and a tumor genome derived from the same individual
- Author
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Fabio C. P. Navarro, Sandro J. de Souza, Lucila Ohno-Machado, Marco Antonio Zago, Luiz Gonzaga Paula de Almeida, Wilson A. Silva, Renato G. Pedigoni, Otavia L. Caballero, Pedro A. F. Galante, Thiago Y. Oliveira, Zhen Ye, Andrew J. G. Simpson, Bing Ren, Alexandra L. Gerber, Ana Paula M Silva, Anamaria A. Camargo, Marcelo G. de Paula, Marisa Fabiana Nicolás, Samantha Kuan, Raphael B. Parmigiani, Daniel Guariz Pinheiro, Robert L. Strausberg, Israel Tojal, Lee Edsall, Sylvie Devalle, Rodrigo Guarischi Mattos Amaral de Sousa, Ewen F. Kirkness, Ana Tereza Ribeiro de Vasconcelos, Jorge Estefano Santana de Souza, Qi Zhao, Anna Christina M. Salim, and Samuel Levy
- Subjects
Somatic cell ,Breast Neoplasms ,Biology ,medicine.disease_cause ,Genome ,03 medical and health sciences ,0302 clinical medicine ,Cell Line, Tumor ,Protein Interaction Mapping ,Genetics ,medicine ,Humans ,Point Mutation ,Lymphocytes ,KEGG ,Gene ,Cell Line, Transformed ,030304 developmental biology ,Chromosome Aberrations ,0303 health sciences ,Mutation ,Genome, Human ,Point mutation ,Genetic Variation ,Genomics ,Sequence Analysis, DNA ,Middle Aged ,030220 oncology & carcinogenesis ,Female ,Human genome ,Carcinogenesis - Abstract
Although patterns of somatic alterations have been reported for tumor genomes, little is known on how they compare with alterations present in non-tumor genomes. A comparison of the two would be crucial to better characterize the genetic alterations driving tumorigenesis. We sequenced the genomes of a lymphoblastoid (HCC1954BL) and a breast tumor (HCC1954) cell line derived from the same patient and compared the somatic alterations present in both. The lymphoblastoid genome presents a comparable number and similar spectrum of nucleotide substitutions to that found in the tumor genome. However, a significant difference in the ratio of non-synonymous to synonymous substitutions was observed between both genomes (P = 0.031). Protein–protein interaction analysis revealed that mutations in the tumor genome preferentially affect hub-genes (P = 0.0017) and are co-selected to present synergistic functions (P
- Published
- 2011
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