1. Kang-Ai Injection Inhibits Gastric Cancer Cells Proliferation through IL-6/STAT3 Pathway
- Author
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Kezuo Hou, Xiaofang Che, Hai-Yan Qi, Yunpeng Liu, Wanxia Fang, Shi-Tong Yu, Jin-E Liang, An-Qi Wang, Chunlei Zheng, and Xiujuan Qu
- Subjects
Cyclin E ,biology ,Kinase ,Chemistry ,Cyclin A ,0211 other engineering and technologies ,02 engineering and technology ,General Medicine ,Cell cycle ,030226 pharmacology & pharmacy ,Molecular biology ,03 medical and health sciences ,0302 clinical medicine ,Cyclin D1 ,Complementary and alternative medicine ,021105 building & construction ,biology.protein ,Pharmacology (medical) ,STAT3 ,Cyclin B1 ,Protein kinase B - Abstract
To explore the mechanisms underlying the proliferative inhibition of Chinese herbal medicine Kang-ai injection (KAI, 康艾注射液) in gastric cancer cells. Gastric cancer cell lines MGC803 and BGC823 were treated by 0, 0.3%, 1%, 3% and 10% KAI for 24, 48 and 72 h, respectively. The cell proliferation was evaluated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay. The apoptosis and cell cycle were evaluated by flow cytometry. Interleukin (IL)-6 mRNA and protein expression levels were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and enzyme-linked immune sorbent assay (ELISA), respectively. The protein expression levels of cyclin A, cyclin E, cyclin B1, cyclin D1, p21, retinoblastoma (RB), protein kinase B (AKT), extracellular regulated protein kinases (ERK), signal transducer and activator of transcription (STAT) 1 and STAT3 were detected by Western blot. KAI inhibited the proliferation of MGC803 and BGC823 gastric cancer cells in dose- and time-dependent manner. After treated with KAI for 48 h, the proportion of G1 phase was increased, expression level of cyclin D1 and phosphorylation-RB were down-regulated, whereas the expression of p21 was up-regulated (all P
- Published
- 2020