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Purpose: Due to a lack of recognized molecular targets for therapy, patients with triple-negative breast cancer (TNBC), unlike other subtypes of breast cancers, generally have not benefited from the advances made with targeted agents. The CXCR4/SDF-1 axis is involved in tumor growth and metastasis of TNBC. Therefore, down-regulation of the expression of CXCR4 in cancer cells is a potential therapeutic strategy for inhibiting primary tumor growth and metastasis of TNBC. In order to identify bioactive compounds that inhibit the expression of CXCR4 in traditional Chinese medicines, we investigated the capacity of saikosaponin A (SSA), one of the active ingredients isolated from Radix bupleuri, to affect CXCR4 expression and function in TNBC cells. Methods: Analyses of cell growth, migration, invasion, and protein expression were performed. Knockdowns by small interfering RNA (siRNA) and non-invasive bioluminescence were also used. Results: SSA reduced proliferation and colony formation of SUM149 and MDA-MB-231 cells. SSA inhibited migration and invasion of TNBC cells. For mice, SSA inhibited primary tumor growth and reduced lung metastasis of highly metastatic, triple-negative 4T1-luc cells. SSA inhibited CXCR4 expression but did not regulate CXCR7 expression in vitro and in vivo. The inhibitory effects on the migration and invasion of TNBC cells were reversed by down-regulation of CXCR4 expression. In addition, SSA inactivated the Akt/mTOR signaling pathway and inhibited MMP-9 and MMP-2 expression. Conclusions: The results show that SSA exerts an anti-TNBC effect through the inhibition of CXCR4 expression and thus has the potential to be a candidate therapeutic agent for TNBC patients.

To explore the clinical application value of computer molecular imaging tracing stem cell transplantation in the treatment of myocardial infarction. Method: 90 patients with myocardial infarction admitted in our hospital from 2014.06 to 2016.06 were selected as subjects of this study, were randomly divided into two groups of 45 cases, the control group was treated with conventional therapy. The observation group was given molecular imaging tracing stem cell transplantation on the basis of conventional therapy. The patients in the two groups were treated for 3 weeks consecutively. After treatment, the patients in the two groups were followed up for 6 months. The total effective rate, left ventricular function index and complication rate after treatment were observed and compared. Results: The total effective rate of the treatment group was 97.8%, which was significantly higher than that of the control group (75.6%). The difference was statistically significant (P 0.05). The incidence of complications in the observation group was 4.4%, which was significantly lower than that in the control group (P