1. Transcription factor MafB in podocytes protects against the development of focal segmental glomerulosclerosis
- Author
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Keigyou Yoh, Ryusuke Koshida, Masafumi Muratani, Hyojung Jeon, Takaaki Ojima, Yuki Imamura, Michito Hamada, Naoki Morito, Maho Kanai, Hideki Yokoi, Manoj Kumar Yadav, Satoru Takahashi, Ashio Yoshimura, Hisashi Oishi, Masato Kasahara, Toshiaki Usui, Keigo Asano, Hossam H. Shawki, Joichi Usui, Yoshinori Sato, Akihiro Kuno, Takashi Kudo, Yuki Tsunakawa, Kunihiro Yamagata, Risa Okada, and Hiroyasu Tsukaguchi
- Subjects
0301 basic medicine ,Nephrotic Syndrome ,MafB Transcription Factor ,030232 urology & nephrology ,Mice, Transgenic ,urologic and male genital diseases ,Podocyte ,Pathogenesis ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Focal segmental glomerulosclerosis ,Basic Helix-Loop-Helix Transcription Factors ,medicine ,Animals ,Humans ,Transcription Factor MafB ,Transcription factor ,Proteinuria ,Glomerulosclerosis, Focal Segmental ,Podocytes ,urogenital system ,business.industry ,medicine.disease ,female genital diseases and pregnancy complications ,030104 developmental biology ,medicine.anatomical_structure ,Nephrology ,MAFB ,Cancer research ,medicine.symptom ,business ,Nephrotic syndrome - Abstract
Focal segmental glomerulosclerosis (FSGS) is a common cause of steroid-resistant nephrotic syndrome. Spontaneous remission of FSGS is rare and steroid-resistant FSGS frequently progresses to renal failure. Many inheritable forms of FSGS have been described, caused by mutations in proteins that are important for podocyte function. Here, we show that a basic leucine zipper transcription factor, MafB, protects against FSGS. MAFB expression was found to be decreased in the podocytes of patients with FSGS. Moreover, conditional podocyte-specific MafB-knockout mice developed FSGS with massive proteinuria accompanied by depletion of the slit diaphragm-related proteins (Nphs1 and Magi2), and the podocyte-specific transcription factor Tcf21. These findings indicate that MafB plays a crucial role in the pathogenesis of FSGS. Consistent with this, adriamycin-induced FSGS and attendant proteinuria were ameliorated by MafB overexpression in the podocytes of MafB podocyte-specific transgenic mice. Thus, MafB could be a new therapeutic target for FSGS.
- Published
- 2020