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Introduction Cervical cancer is the fourth most common cancer in females demonstrating approximately more than 7% of all woman cancer mortality in 2018. HPV infection considered as the pivotal risk factor for cervical cancer induction. Permanent infection with carcinogenic genital HPVs increases the predisposition of cervical cancer. In this study, we investigated the frequency of HPV genotypes in a sample of Iranian population. Methods Therefore, 2135 individuals with genital warts between 2015 and 2018 recruited to the study. After DNA extraction, PCR amplification and genotyping were employed by HPV Direct Flow CHIP Kit and the e-BRID System®, supported by hybriSoft ™. HPV was found in 92% of the samples obtained from the patients. Results In total, 12.6% and 87.4% of warts were positive for any high-risk and low-risk genotypes, respectively. HPV 16 and 6 were the most predominant HR and LR genotypes in all categorized age groups (5.26% and 40.71%, respectively). Moreover, by getting older, the frequency of HPV 6 was decreased (P = 0 .018, r = −0.050) and the frequency of HPV 56 was increased (P =0 .026, r = 0.044). Conclusion Although we noticed lower prevalence of high risk HPV particularly 16 and 18 in genital warts in the East of Iran; however, genotyping of genital warts still recommended.

Background: Infection by certain types of human papilloma virus (HPV) is known as a causal and essential factor for cervical cancer, the second most common malignancy in women around the world. Objectives: The aim of this study was to determine the frequency and types of HPV among women with normal and abnormal cytology in Southern Khorasan, eastern Iran. Methods: This was a cross-sectional study with 253 randomized Pap smear samples from women who were referred to gynecologist clinics. Human papillomavirus-DNA testing (a nested PCR with primers MY09/ MY11 and GP5 +/ GP6 +) was performed on Pap smear samples. The first round PCR product was subjected for sequencing to determine the HPV types. Phylogenic analysis with Mega 6 was carried out to determine the relationship between HPV types. Results: The mean age of patients were 34.47 ± 5.38 years; 85.77% with normal cytology, and the rest were with an abnormality; atypical cells of undetermined significance (ASCUS) and Low-grade squamous intraepithelial lesions (LISL). Human papilloma virus-DNA was detected in 18.57% of population (15.66% of normal and 36.11% of abnormal group) with the most prevalent HPV types 6 and 11. The HPV type 84 was identified in a case. Conclusions: The result of this study revealed a partially high prevalence of HPV in women with normal cytology which are high risk for transmission in population. It is suggested that HPV testing should be carried out along with Pap test in screening programs to enhance early detection of neoplasia and intended infections.