1. Sequence and annotation of the Wizard007 mycobacterium phage genome
- Author
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Anthony Falcone, Jordan Olberding, Mackenzie Perkins, Sarah Schrader, Karlee Driver, Courtney Howard, Prasanna Tamarapu Parthasarathy, Heidi Sayre, Cynthia A. Tope, Benjamin Howard, Tyler Scaff, Ejike Anyanwu, Elizabeth Farnsworth, Kaitlyn Cole, Brittney Howard, Rodney A. King, and Claire A. Rinehart
- Subjects
Computational biology ,Biology ,lcsh:Computer applications to medicine. Medical informatics ,Bioinformatics ,Genome ,Biochemistry ,DNA sequencing ,Bacteriophage ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Structural Biology ,lcsh:QH301-705.5 ,Gene ,Molecular Biology ,030304 developmental biology ,0303 health sciences ,Applied Mathematics ,biology.organism_classification ,DNA extraction ,Computer Science Applications ,genomic DNA ,lcsh:Biology (General) ,chemistry ,Poster Presentation ,lcsh:R858-859.7 ,DNA microarray ,030217 neurology & neurosurgery ,DNA - Abstract
Material and methods The Wizard007 bacteriophage was isolated from a soil sample taken at N36° 49’36.8” W87° 29’42.5” in Hopkinsville, KY. It was plaque purified on Mycobacterium smegmatis and DNA was isolated using the Promega Wizard DNA purification kit. The DNA was sequenced at the Virginia Commonwealth University by Roche 454 DNA sequencing. The genome quality was confirmed in Consed [1] and found to be 51,034 bp in length. Genes were called from the assembled sequence with a workflow consisting of Glimmer [2], GeneMark [3], tRNAscan [4], and SDFinder to identify potential gene features. Gbrowse [5], Apollo [6], and BLAST [7] were used to call and annotate genes. A 10 base 3’ overhang was found at the ends by ligating the genomic DNA and sequencing a PCR product produced across the ligated ends.
- Published
- 2010