Author: "Isabelle Rivals" / Topic: 030217 neurology & neurosurgery - Searchworks@Jio Institute Digital Library Search Results

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27 results on '"Isabelle Rivals"'

1. Ultrastructural and dynamic studies of the endosomal compartment in Down syndrome

Author: Alexandra Botté, Jeanne Lainé, Xavier Heiligenstein, Marie-Claude Potier, Graça Raposo, Gaëlle Fontaine, Isabelle Rivals, Anne-Sophie Rebillat, Dean Nizetic, Jack-Christophe Cossec, Etienne Morel, Laura Xicota, Pollyanna Goh, Orestis Faklaris, Amal Kasri, Institut du Cerveau et de la Moëlle Epinière = Brain and Spine Institute (ICM), Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Biologie Cellulaire et Cancer, Institut Curie [Paris]-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Neurophysiologie Respiratoire Expérimentale et Clinique, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL), Equipe de Statistique Appliquée (UMRS 1158) (ESA), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Barts and the London Medical School, Queen Mary University of London (QMUL), Institut Jacques Monod (IJM (UMR_7592)), Université de Paris (UP)-Centre National de la Recherche Scientifique (CNRS), Institut Necker Enfants-Malades (INEM - UM 111 (UMR 8253 / U1151)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Institut Jérôme Lejeune, Nanyang Technological University [Singapour], Lee Kong Chian School of Medicine (LKCMedicine), Gestionnaire, Hal Sorbonne Université, Institut du Cerveau = Paris Brain Institute (ICM), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Neurophysiologie Respiratoire Expérimentale et Clinique (UMRS 1158), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique (UMRS 1158), Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), and Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)
Subjects: Tissue Fixation, Endosome, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Down syndrome, Induced Pluripotent Stem Cells, Endosomes, Immunofluorescence, Endocytosis, lcsh:RC346-429, Pathology and Forensic Medicine, law.invention, EEA1, Mice, 03 medical and health sciences, Cellular and Molecular Neuroscience, 0302 clinical medicine, Microscopy, Electron, Transmission, law, Confocal microscopy, Early endosomes, medicine, Electron microscopy, Animals, Humans, Medicine [Science], Alzheimer’s Disease, Cholinergic neuron, Super-resolution microscopy, lcsh:Neurology. Diseases of the nervous system, 030304 developmental biology, 0303 health sciences, Microscopy, Confocal, medicine.diagnostic_test, Chemistry, Research, [SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Fibroblasts, Alzheimer's disease, Vitrification, Cell biology, Ultrastructure, Neurology (clinical), Down Syndrome, Electron microscope, Alzheimer’s disease, 030217 neurology & neurosurgery
Abstract: Enlarged early endosomes have been visualized in Alzheimer's disease (AD) and Down syndrome (DS) using conventional confocal microscopy at a resolution corresponding to endosomal size (hundreds of nm). In order to overtake the diffraction limit, we used super-resolution structured illumination microscopy (SR-SIM) and transmission electron microscopies (TEM) to analyze the early endosomal compartment in DS.By immunofluorescence and confocal microscopy, we confirmed that the volume of Early Endosome Antigen 1 (EEA1)-positive puncta was 13-19% larger in fibroblasts and iPSC-derived neurons from individuals with DS, and in basal forebrain cholinergic neurons (BFCN) of the Ts65Dn mice modelling DS. However, EEA1-positive structures imaged by TEM or SR-SIM after chemical fixation had a normal size but appeared clustered. In order to disentangle these discrepancies, we imaged optimally preserved High Pressure Freezing (HPF)-vitrified DS fibroblasts by TEM and found that early endosomes were 75% denser but remained normal-sized.RNA sequencing of DS and euploid fibroblasts revealed a subgroup of differentially-expressed genes related to cargo sorting at multivesicular bodies (MVBs). We thus studied the dynamics of endocytosis, recycling and MVB-dependent degradation in DS fibroblasts. We found no change in endocytosis, increased recycling and delayed degradation, suggesting a "traffic jam" in the endosomal compartment.Finally, we show that the phosphoinositide PI (3) P, involved in early endosome fusion, is decreased in DS fibroblasts, unveiling a new mechanism for endosomal dysfunctions in DS and a target for pharmacotherapy. National Research Foundation (NRF) Published version Marie-Claude Potier was supported by Fondation Vaincre Alzheimer (fellowship to AB), Fondation Jérôme Lejeune and Agence Nationale de laRecherche (ANR-10-IAIHU-06). Dean Nizetic was funded by Wellcome Trust (098330/Z/12/Z), NationalResearch Foundation Singapore (NMRC/CS-IRG/1438/2015), the WellcomeTrust Collaborative Award in Science 217199/Z/19/Z, and by the “Research Cooperability“ Programme of the Croatian Science Foundation (PZS-2019-02-4277). France-BioImaging infrastructure was supported by the French National Research Agency (ANR-10-INBS-04)..
Published: 2020
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2. Evening sock marks as an adjunct to the clinical prediction of obstructive sleep apnea

Author: Elisa Perger, Carole Philippe, Oumama Badarani, Thomas Similowski, Stefania Redolfi, Isabelle Arnulf, Isabelle Rivals, Perger, E, Badarani, O, Philippe, C, Rivals, I, Arnulf, I, Similowski, T, and Redolfi, S
Subjects: Male, medicine.medical_specialty, Evening, Polysomnography, Fluid shift, Clothing, 03 medical and health sciences, 0302 clinical medicine, Interquartile range, Internal medicine, medicine, Humans, Sleep study, Fluid Shifts, Morning, Sleep Apnea, Obstructive, medicine.diagnostic_test, Ventilatory polygraphy, business.industry, Middle Aged, medicine.disease, Obstructive sleep apnea, nervous system diseases, respiratory tract diseases, 030228 respiratory system, Otorhinolaryngology, Apnea–hypopnea index, Cardiology, Female, Neurology (clinical), business, Body mass index, Biomarkers, 030217 neurology & neurosurgery, Diagnosi
Abstract: Study objectives: Fluid overload shifting from the legs to the upper airway during sleep promotes obstructive sleep apnea (OSA) and interventions targeting fluid attenuate OSA. Fluid shift has been previously measured by bioelectrical impedance, a complex and time-consuming technique not applicable in the daily clinical settings. The aim of this study is to evaluate the presence of clinically detectable fluid overload and shift and its association with OSA. Methods: Patients undergoing sleep study for suspected OSA were asked to report the presence of 11 signs/symptoms associated to excessive accumulation of fluid in different parts of the body at different times of the day. Results: Among 392 patients (male: 53%, median [interquartile range] age: 56years [1], body mass index, BMI: 29kg/m2 [2]) included in the study, 135 (34%) had moderate-to-severe OSA (apnea hypopnea index, AHI ≥ 15). Daytime fluid accumulation and nocturnal fluid shift, clinically detectable by patient-reported “evening sock marks,” “heavy legs during the day,” and “morning stuffed nose,” were prevalent in the entire population (46%, 43%, and 33%, respectively). In multivariate analysis, evening sock marks was an independent correlate of having an AHI ≥ 15, together with male sex, older age, and self-reported snoring and apneas. Conclusions: Clinically detectable fluid overload and shift are prevalent in patients addressed for suspected OSA, and evening sock marks, a marker for leg swelling, is an independent correlate of moderate-to-severe OSA. This sign might contribute to OSA diagnosis and identification of patients likely to be treated by interventions targeting fluid overload and shift.
Published: 2019
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3. Reply to ‘Letter to the editor: is maximal diaphragm tissue velocity suited for the assessment of diaphragm contractility?’

Author: Damien Bachasson, Marie-Cécile Niérat, Thomas Poulard, Jean-Yves Hogrel, Jean-Luc Gennisson, Isabelle Rivals, Martin Dres, Thomas Similowski, Institut de Myologie, Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Association française contre les myopathies (AFM-Téléthon)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Unité BioMaps (BIOMAPS), Service Hospitalier Frédéric Joliot (SHFJ), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Service de Pneumologie et Réanimation Médicale [CHU Pitié-Salpêtrière] (Département ' R3S '), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Neurophysiologie Respiratoire Expérimentale et Clinique (UMRS 1158), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Equipe de Statistique Appliquée (UMRS 1158) (ESA), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique (UMRS 1158), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Neurophysiologie Respiratoire Expérimentale et Clinique, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique, LaBoratoire d'Imagerie biOmédicale MultimodAle Paris-Saclay (BIOMAPS), and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)
Subjects: MESH: Phrenic Nerve, ultrafast ultrasound imaging, MESH: Magnetic Phenomena, Physiology, MESH: Muscle Contraction, Diaphragm (mechanical device), contractility, Contractility, Magnetics, 03 medical and health sciences, 0302 clinical medicine, phrenic nerves, Medicine, MESH: Magnetics, skeletal muscle, ComputingMilieux_MISCELLANEOUS, Tissue velocity, business.industry, Magnetic Phenomena, Skeletal muscle, Anatomy, cervical magnetic stimulation, Phrenic Nerve, medicine.anatomical_structure, 030228 respiratory system, diaphragm, MESH: Diaphragm, business, 030217 neurology & neurosurgery, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, Muscle Contraction
Abstract: International audience
Published: 2021
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4. Clinical features and outcome of patients with primary central nervous system lymphoma admitted to the intensive care unit: a French national expert center experience

Author: Caroline Houillier, Isabelle Rivals, Nicolas Gatulle, Charles-Edouard Luyt, Maxens Decavèle, Nicolas Weiss, Thomas Similowski, Aliénor Dreyfus, Julie Delemazure, Khê Hoang-Xuan, Elise Morawiec, Julien Mayaux, Sophie Demeret, Alexandre Demoule, Sylvain Choquet, Martin Dres, Gestionnaire, HAL Sorbonne Université 5, Service de Pneumologie et Réanimation Médicale [CHU Pitié-Salpêtrière] (Département ' R3S '), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Service de Neurologie [CHU Pitié-Salpêtrière], IFR70-CHU Pitié-Salpêtrière [AP-HP], Institut de neurosciences translationnelles de Paris (NeurATRIS - IHU-A-ICM), Institut du Cerveau = Paris Brain Institute (ICM), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Centre d'investigation clinique Neurosciences [CHU Pitié Salpêtrière] (CIC Neurosciences), Centre d'investigation clinique pluridisciplinaire [CHU Pitié Salpêtrière] (CIC-P 1421), Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Neurophysiologie Respiratoire Expérimentale et Clinique (UMRS 1158), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Institut de cardiologie [CHU Pitié-Salpêtrière], Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Research Unit on Cardiovascular and Metabolic Diseases (ICAN), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut de Cardiométabolisme et Nutrition = Institute of Cardiometabolism and Nutrition [CHU Pitié Salpêtrière] (IHU ICAN), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL), Service d'Hématologie clinique [CHU Pitié-Salpêtrière], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Neurophysiologie Respiratoire Expérimentale et Clinique, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Institute of cardiometabolism and nutrition (ICAN), Institut du Cerveau et de la Moëlle Epinière = Brain and Spine Institute (ICM), Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Central Nervous System, medicine.medical_specialty, Neurology, Lymphoma, medicine.medical_treatment, Aspiration pneumonia, [SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract, law.invention, 03 medical and health sciences, 0302 clinical medicine, law, Neuro-oncology, medicine, Humans, Intensive care medicine, Hospital Mortality, 030212 general & internal medicine, Primary central nervous system lymphoma, Retrospective Studies, Mechanical ventilation, Coma, Original Communication, business.industry, Prognosis, medicine.disease, Triage, Intensive care unit, 3. Good health, Hospitalization, Intensive Care Units, Critical care, Emergency medicine, [SDV.MHEP.PSR] Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract, Neurology (clinical), medicine.symptom, business, 030217 neurology & neurosurgery, Cohort study
Abstract: Introduction To describe the reasons for intensive care unit (ICU) admission and to evaluate the outcomes and prognostic factors of patients with primary central nervous system lymphoma (PCNSL) admitted to the ICU. Patients and methods Retrospective observational cohort study of 101 PCNSL patients admitted to 3 ICUs over a two-decade period. Results Acute respiratory failure, mainly secondary to aspiration pneumonia and Pneumocystis jirovecii pneumonia, was the leading reason for ICU admission (33%). Aspiration pneumonia was more common in patients with brainstem tumor (67% vs. 0%, p
Published: 2021
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5. Ultrafast ultrasound coupled with cervical magnetic stimulation for non‐invasive and non‐volitional assessment of diaphragm contractility

Author: Jean-Yves Hogrel, Martin Dres, Thomas Poulard, Marie-Cécile Niérat, Damien Bachasson, Thomas Similowski, Isabelle Rivals, Jean-Luc Gennisson, LaBoratoire d'Imagerie biOmédicale MultimodAle Paris-Saclay (BIOMAPS), Service Hospitalier Frédéric Joliot (SHFJ), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de Myologie, Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Association française contre les myopathies (AFM-Téléthon)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Neurophysiologie Respiratoire Expérimentale et Clinique (UMRS 1158), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL), dres, martin, Equipe de Statistique Appliquée (UMRS 1158) (ESA), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique (UMRS 1158), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), HAL-SU, Gestionnaire, Service de Pneumologie et Réanimation Médicale [CHU Pitié-Salpêtrière] (Département ' R3S '), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Unité BioMaps (BIOMAPS), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Neurophysiologie Respiratoire Expérimentale et Clinique, Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique
Subjects: 0301 basic medicine, ultrafast ultrasound imaging, [SDV.MHEP.PHY] Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Physiology, [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, [SDV]Life Sciences [q-bio], Stimulation, contractility, Contractility, 03 medical and health sciences, 0302 clinical medicine, phrenic nerves, [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Medicine, skeletal muscle, business.industry, Non invasive, Ultrasound, Skeletal muscle, cervical magnetic stimulation, 3. Good health, Diaphragm (structural system), Intensity (physics), [SDV] Life Sciences [q-bio], [SDV.IB.IMA] Life Sciences [q-bio]/Bioengineering/Imaging, 030104 developmental biology, medicine.anatomical_structure, diaphragm, medicine.symptom, business, 030217 neurology & neurosurgery, Biomedical engineering, Muscle contraction
Abstract: International audience; Key points: Twitch transdiaphragmatic pressure elicited by cervical magnetic stimulation of the phrenic nerves is a fully non-volitional method for assessing diaphragm contractility in humans, yet it requires invasive procedures such as oesophageal and gastric catheter balloons. Ultrafast ultrasound enables a very high frame rate allowing the capture of transient events, such as muscle contraction elicited by nerve stimulation (twitch). Whether indices derived from ultrafast ultrasound can be used as an alternative to the invasive measurement of twitch transdiaphragmatic pressure is unknown. Our findings demonstrate that maximal diaphragm tissue velocity assessed using ultrafast ultrasound following cervical magnetic stimulation is reliable, sensitive to change in cervical magnetic stimulation intensity, and correlates to twitch transdiaphragmatic pressure. This approach provides a novel fully non-invasive and non-volitional tool for the assessment of diaphragm contractility in humans.Abstract: Measuring twitch transdiaphragmatic pressure (Pdi,tw ) elicited by cervical magnetic stimulation (CMS) is considered as a reference method for the standardized evaluation of diaphragm function. Yet, the measurement of Pdi requires invasive oesophageal and gastric catheter-balloons. Ultrafast ultrasound is a non-invasive imaging technique enabling frame rates high enough to capture transient events such as evoked muscle contractions. This study investigated relationships between indices derived from ultrafast ultrasound and Pdi,tw , and how these indices might be used to estimate Pdi,tw . CMS was performed in 13 healthy volunteers from 30% to 100% of maximal stimulator intensity in units of 10% in a randomized order. Pdi,tw was measured and the right hemidiaphragm was imaged using a custom ultrafast ultrasound sequence with 1 kHz framerate. Maximal diaphragm axial velocity (Vdi ,max ) and diaphragm thickening fraction (TFdi,tw ) were computed. Intra-session reliability was assessed. Repeated-measures correlation (R) and Spearman correlation coefficients (ρ) were used to assess relationships between variables. Intra-session reliability was strong for Pdi,tw and Vdi,max and moderate for TFdi,tw . Vdi,max correlated with Pdi,tw in all subjects (0.64 < ρ < 1.00, R = 0.75; all P < 0.05). TFdi,tw correlated with Pdi,tw in eight subjects only (0.85 < ρ < 0.93, R = 0.69; all P < 0.05). Coupling ultrafast ultrasound and CMS shows promise for the non-invasive and fully non-volitional assessment of diaphragm contractility. This approach opens up the prospect of both diagnosis and follow-up of diaphragm contractility in clinical populations.
Published: 2020
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6. One-year survival of patients with high-grade glioma discharged alive from the intensive care unit

Author: Isabelle Rivals, Khê Hoang-Xuan, Martin Dres, Sophie Demeret, Nicolas Weiss, Ahmed Idbaih, Elise Morawiec, Julien Mayaux, Nicolas Gatulle, Maxens Decavèle, Alexandre Demoule, and Thomas Similowski
Subjects: medicine.medical_specialty, Critical Care, One-year survival, law.invention, 03 medical and health sciences, 0302 clinical medicine, law, Intensive care, Glioma, Internal medicine, medicine, Humans, Intensive care unit, 030212 general & internal medicine, Hospital Mortality, Retrospective Studies, Original Communication, Performance status, business.industry, Mortality rate, Anticancer therapy, Cancer, Odds ratio, medicine.disease, Malignant brain tumors, Patient Discharge, Intensive Care Units, Neurology, Neurology (clinical), business, 030217 neurology & neurosurgery, Cohort study
Abstract: Introduction Only limited data are available regarding the long-term prognosis of patients with high-grade glioma discharged alive from the intensive care unit. We sought to quantify 1-year mortality and evaluate the association between mortality and (1) functional status, and (2) management of anticancer therapy in patients with high-grade glioma discharged alive from the intensive care unit. Patients and methods Retrospective observational cohort study of patients with high-grade glioma admitted to two intensive care units between January 2009 and June 2018. Functional status was assessed by the Karnofsky Performance Status. Anticancer therapy after discharge was classified as (1) continued (unchanged), (2) modified (changed or stopped), or (3) initiated (for newly diagnosed disease). Results Ninety-one high-grade glioma patients (73% of whom had glioblastoma) were included and 78 (86%) of these patients were discharged alive from the intensive care unit. Anticancer therapy was continued, modified, and initiated in 41%, 42%, and 17% of patients, respectively. Corticosteroid therapy at the time of ICU admission [odds ratio (OR) 0.07] and cancer progression (OR 0.09) was independently associated with continuation of anticancer therapy. The mortality rate 1 year after ICU admission was 73%. On multivariate analysis, continuation of anticancer therapy (OR 0.18) and Karnofsky performance status on admission (OR 0.90) were independently associated with lower 1-year mortality. Conclusion The presence of high-grade glioma is not sufficient to justify refusal of intensive care unit admission. Performance status and continuation of anticancer therapy are associated with higher survival after intensive care unit discharge. Previous presentation Preliminary results were presented at the most recent congress of the French Intensive Care Society, Paris, 2019. Electronic supplementary material The online version of this article (10.1007/s00415-020-10191-0) contains supplementary material, which is available to authorized users.
Published: 2020

7. Is a simplified Finite Element model of the gluteus region able to capture the mechanical response of the internal soft tissues under compression?

Author: Pierre-Yves Rohan, Hélène Pillet, Alexandre Verney, Aurélien Macron, Mohammad Sadeghinia, Jennifer Doridam, Isabelle Rivals, Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut de Biomecanique Humaine Georges Charpak, Université Paris 13 (UP13)-Arts et Métiers ParisTech, Equipe de Statistique Appliquée (UMRS 1158) (ESA), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Neurophysiologie Respiratoire Expérimentale et Clinique, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Laboratoire d'Intégration des Systèmes et des Technologies (LIST), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique (UMRS 1158), Neurophysiologie Respiratoire Expérimentale et Clinique (UMRS 1158), and Laboratoire d'Intégration des Systèmes et des Technologies (LIST (CEA))
Subjects: Adult, Male, Risk, Compressive Strength, Computer science, Subject specific, Computation, Finite Element Analysis, Biophysics, Deep Tissue Injury, Models, Biological, Young Adult, 03 medical and health sciences, Imaging, Three-Dimensional, 0302 clinical medicine, Ischium, Position (vector), Image Processing, Computer-Assisted, Pressure, Internal response, Humans, Orthopedics and Sports Medicine, Muscle, Skeletal, Ultrasonography, Pressure Ulcer, Sitting Position, Soft tissue, 030229 sport sciences, Clinical routine, Compression (physics), Finite element method, Buttock, Sciences du vivant, Buttocks, Female, [SDV.IB]Life Sciences [q-bio]/Bioengineering, Stress, Mechanical, Fe model, Shear Strength, Biological system, 030217 neurology & neurosurgery, Sitting
Abstract: Internal soft tissue strains have been shown to be one of the main factors responsible for the onset of Pressure Ulcers and to be representative of its risk of development. However, the estimation of this parameter using Finite Element (FE) analysis in clinical setups is currently hindered by costly acquisition, reconstruction and computation times. Ultrasound (US) imaging is a promising candidate for the clinical assessment of both morphological and material parameters. Method: The aim of this study was to investigate the ability of a local FE model of the region beneath the ischium with a limited number of parameters to capture the internal response of the gluteus region predicted by a complete 3D FE model. 26 local FE models were developed, and their predictions were compared to those of the patient-specific reference FE models in sitting position. Findings: A high correlation was observed (R = 0.90, p-value < 0.01). A sensitivity analysis showed that the most influent parameters were the mechanical behaviour of the muscle tissues, the ischium morphology and the external mechanical loading. Interpretation: Given the progress of US for capturing both morphological and material parameters, these results are promising because they open up the possibility to use personalised simplified FE models for risk estimation in daily clinical routine. This work was supported by the Fondation de l'Avenir (grant number AP-RM-2016-030), by la Fondation des Arts et Métiers and the Fonds de dotation Clinatec. The authors are also grateful to the ParisTech BiomecAM chair program on subject-specific musculoskeletal modelling.
Published: 2020
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8. Decreased respiratory-related postural perturbations at the cervical level under cognitive load

Author: Louis Clavel, Isabelle Rivals, Marie-Cécile Niérat, Valérie Attali, Philippe Rouch, Baptiste Sandoz, Pierantonio Laveneziana, Thomas Similowski, Institut de Biomécanique Humaine Georges Charpak (IBHGC), Université Sorbonne Paris Nord-Arts et Métiers Sciences et Technologies, HESAM Université (HESAM)-HESAM Université (HESAM), INSERM, U708, Neuroepidemiology, F-75005, Paris, France, This work was funded by the Chancellerie des Universités de Paris (Grant number: Legs Poix (LEG 1604)), ENS Cachan (Grant number: Ph.D. fellowship), Assistance Publique - Hôpitaux de Paris (Grant number: Grant 'poste d’accueil APHP/Arts et Métiers' délé-gation à la Recherche Clinique et à l’Innovation (DRCI)), and Paristech (Grant number: BiomeCAM chair).
Subjects: Posturo-ventilatory interaction, medicine.medical_specialty, Respiratory rate, Physiology, [SDV]Life Sciences [q-bio], Kinematics, Centre of pressure, 03 medical and health sciences, 0302 clinical medicine, Physical medicine and rehabilitation, Cog, Postural control, Physiology (medical), Cervical spine, Medicine, Orthopedics and Sports Medicine, Respiratory system, Balance (ability), business.industry, Public Health, Environmental and Occupational Health, Joint kinematics, 030229 sport sciences, General Medicine, Optoelectronic plethysmography, Cognitive dual task, Breathing, Sciences du vivant, business, 030217 neurology & neurosurgery, Cognitive load
Abstract: PURPOSE: In healthy humans, postural and respiratory dynamics are intimately linked and a breathing-related postural perturbation is evident in joint kinematics. A cognitive dual-task paradigm that is known to induce both postural and ventilatory disturbances can be used to modulate this multijoint posturo-ventilatory (PV) interaction, particularly in the cervical spine, which supports the head. The objective of this study was to assess this modulation. METHODS: With the use of optoelectronic sensors, the breathing profile, articular joint motions of the cervical spine, hip, knees and ankles, and centre of pressure (CoP) displacement were measured in 20 healthy subjects (37 years old [29; 49], 10 females) during natural breathing (NB), a cognitive dual task (COG), and eyes-closed and increased-tidal-volume conditions. The PV interaction in the CoP and joint motions were evaluated by calculating the respiratory emergence (REm). RESULTS: Only the COG condition induced a decrease in the cervical REm (NB: 17.2% [7.8; 37.2]; COG: 4.2% [1.8; 10.0] p = 0.0020) concurrent with no changes in the cervical motion. The CoP REm (NB: 6.2% [3.8; 10.3]; COG: 12.9% [5.8; 20.7] p = 0.0696) and breathing frequency (NB: 16.6 min-1 [13.3; 18.7]; COG: 18.6 min-1 [16.3; 19.4] p = 0.0731) tended to increase, while the CoP (p = 0.0072) and lower joint motion displacements (p
Published: 2020
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9. Human diaphragm atrophy in amyotrophic lateral sclerosis is not predicted by routine respiratory measures

Author: Christian Straus, Gaëlle Bruneteau, Fabrice Menegaux, Thomas Similowski, François Salachas, David Akbar, Raquel Guimarães-Costa, Mai Thao Bui, Norma B. Romero, Capucine Morélot-Panzini, Isabelle Rivals, Jésus Gonzalez-Bermejo, Marie-Cécile Niérat, Patrick P. Michel, Centre de recherche en Myologie – U974 SU-INSERM, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Service de Pneumologie et Réanimation Médicale [CHU Pitié-Salpêtrière] (Département ' R3S '), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Equipe de Statistique Appliquée (UMRS 1158) (ESA), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique (UMRS 1158), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Neurophysiologie Respiratoire Expérimentale et Clinique (UMRS 1158), Institut de Myologie, Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Association française contre les myopathies (AFM-Téléthon)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Institut du Cerveau et de la Moëlle Epinière = Brain and Spine Institute (ICM), Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Service de Chirurgie Générale, Viscérale et Endocrinienne [CHU Pitié-Sapêtrière], Service de Neurologie [CHU Pitié-Salpêtrière], IFR70-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique, Neurophysiologie Respiratoire Expérimentale et Clinique, Centre National de la Recherche Scientifique (CNRS)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Association française contre les myopathies (AFM-Téléthon)-Sorbonne Université (SU), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche en Myologie, Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Service de neurologie 1 [CHU Pitié-Salpétrière], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Institut du Cerveau = Paris Brain Institute (ICM), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Pulmonary and Respiratory Medicine, Male, medicine.medical_specialty, Biopsy, [SDV]Life Sciences [q-bio], Diaphragm, Vital Capacity, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, 03 medical and health sciences, 0302 clinical medicine, Functional residual capacity, Atrophy, Internal medicine, medicine, Respiratory muscle, Humans, Amyotrophic lateral sclerosis, Respiratory system, Electrodes, ComputingMilieux_MISCELLANEOUS, Ultrasonography, Muscle Weakness, business.industry, Respiration, [SDV.BA]Life Sciences [q-bio]/Animal biology, Amyotrophic Lateral Sclerosis, Middle Aged, [SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences, musculoskeletal system, medicine.disease, Respiratory Muscles, Diaphragm (structural system), Respiratory Function Tests, Diaphragm pacing, 030228 respiratory system, Respiratory failure, Adipose Tissue, Cardiology, Regression Analysis, Female, business, Respiratory Insufficiency, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, 030217 neurology & neurosurgery
Abstract: Amyotrophic lateral sclerosis (ALS) patients show progressive respiratory muscle weakness leading to death from respiratory failure. However, there are no data on diaphragm histological changes in ALS patients and how they correlate with routine respiratory measurements.We collected 39 diaphragm biopsies concomitantly with laparoscopic insertion of intradiaphragmatic electrodes during a randomised controlled trial evaluating early diaphragm pacing in ALS (https://clinicaltrials.gov; NCT01583088). Myofibre type, size and distribution were evaluated by immunofluorescence microscopy and correlated with spirometry, respiratory muscle strength and phrenic nerve conduction parameters. The relationship between these variables and diaphragm atrophy was assessed using multivariate regression models.All patients exhibited significant slow- and fast-twitch diaphragmatic atrophy. Vital capacity (VC), maximal inspiratory pressure, sniff nasal inspiratory pressure (SNIP) and twitch transdiaphragmatic pressure did not correlate with the severity of diaphragm atrophy. Inspiratory capacity (IC) correlated modestly with slow-twitch myofibre atrophy. Supine fall in VC correlated weakly with fast-twitch myofibre atrophy. Multivariate analysis showed that IC, SNIP and functional residual capacity were independent predictors of slow-twitch diaphragmatic atrophy, but not fast-twitch atrophy.Routine respiratory tests are poor predictors of diaphragm structural changes. Improved detection of diaphragm atrophy is essential for clinical practice and for management of trials specifically targeting diaphragm muscle function.
Published: 2019
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10. Mandibular advancement reveals long-term suppression of breathing discomfort in patients with obstructive sleep apnea syndrome

Author: Capucine Morélot-Panzini, Valérie Attali, Isabelle Arnulf, Sandie Souchet, Jean-Baptiste Kerbrat, Olivier Jacq, Isabelle Rivals, Patrick Goudot, Thomas Similowski, Jean-Marc Collet, Service des Explorations des Pathologies du Sommeil [CHU Pitié-Salpêtrière], Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-CHU Pitié-Salpêtrière [APHP], Neurophysiologie Respiratoire Expérimentale et Clinique, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Service de Stomatologie et Chirurgie Maxillo-facial [CHU Pitié-Salpêtrière], Statistique, Analyse et Modélisation Multidisciplinaire (SAmos-Marin Mersenne) (SAMM), Université Panthéon-Sorbonne (UP1), Equipe de Statistique Appliquée (ESA), ESPCI ParisTech, Adaptation Biologique et Vieillissement = Biological Adaptation and Ageing (B2A), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Service de Pneumologie et Réanimation Médicale [CHU Pitié-Salpêtrière] (Département ' R3S '), Service des Pathologies du sommeil [CHU Pitié-Salpêtrière], CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Université Paris 1 Panthéon-Sorbonne (UP1), Equipe de Statistique Appliquée (UMRS 1158) (ESA), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service de Pathologies du sommeil [CHU Pitié-Salpêtrière], Neurophysiologie Respiratoire Expérimentale et Clinique (UMRS 1158), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique (UMRS 1158), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), and Gestionnaire, Hal Sorbonne Université
Subjects: Pulmonary and Respiratory Medicine, Adult, Male, Supine position, animal structures, [SDV.MHEP.PHY] Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Physiology, Posture, Sitting, [SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract, 03 medical and health sciences, 0302 clinical medicine, stomatognathic system, Obstructive sleep apnoea syndrome, Mandibular advancement device, [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Medicine, Humans, In patient, Respiratory system, Habituation, Psychophysiologic, Sensory habituation, Sleep Apnea, Obstructive, business.industry, General Neuroscience, Middle Aged, medicine.disease, Critical closing pressure, respiratory tract diseases, Obstructive sleep apnea, Gating out, Dyspnea, 030228 respiratory system, Anesthesia, Latent dyspnoea, Breathing, [SDV.MHEP.PSR] Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract, Female, Breathing discomfort, business, Mandibular Advancement, 030217 neurology & neurosurgery
Abstract: International audience; Obstructive sleep apnoea syndrome (OSAS) patients do not report breathing discomfort in spite of abnormal upper airway mechanics. We studied respiratory sensations in OSAS patients without and with mandibular advancement device (MAD).Fifty-seven moderate to severe non obese OSAS patients were asked about breathing discomfort using visual analogue scales (VAS) in the sitting position (VAS-1), after lying down (VAS-2), then with MAD (VAS-3). Awake critical closing pressure (awake Pcrit) was measured in 15 patients without then with MAD.None of the patients reported breathing discomfort when sitting but 19 patients (33%) did when lying (VAS-2: -20% or less). A feeling of "easier breathing" with MAD was observed and was more marked in patients reporting breathing discomfort when supine (VAS-3: +66.0% [49.0; 89.0]) than in those not doing so (VAS-3: +28.5% [1.0; 56.5], p = 0.007). MAD-induced change in awake Pcrit was correlated to VAS-3.In conclusion, MAD revealed “latent dyspnea” related to the severity of upper airways mechanics abnormalities in OSAS patients.
Published: 2019
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11. Etiology and prognosis of acute respiratory failure in patients with primary malignant brain tumors admitted to the intensive care unit

Author: Léa Lemasle, Thomas Similowski, Isabelle Rivals, Marie Cantier, Alexandre Demoule, Ahmed Idbaih, Clémence Marois, Khê Hoang-Xuan, Maxens Decavèle, Hélène Prodanovic, and Nicolas Weiss
Subjects: Male, Cancer Research, medicine.medical_specialty, Acute infectious pneumonia, Aspiration pneumonia, Pneumocystis pneumonia, Pneumonia, Aspiration, law.invention, 03 medical and health sciences, 0302 clinical medicine, law, Internal medicine, medicine, Humans, Aged, Retrospective Studies, business.industry, Brain Neoplasms, Pneumonia, Pneumocystis, Cancer, Middle Aged, medicine.disease, Prognosis, Intensive care unit, Pulmonary embolism, Survival Rate, Intensive Care Units, Neurology, Oncology, Respiratory failure, 030220 oncology & carcinogenesis, Case-Control Studies, Etiology, Female, Neurology (clinical), business, Respiratory Insufficiency, 030217 neurology & neurosurgery
Abstract: Acute respiratory failure (ARF) is common and potentially fatal in patients with primary malignant brain tumors (PMBT). However, few data are available regarding its precipitating factors and prognosis. We sought to: (1) compare the causes of ARF and the outcome between patients with PMBT and patients with other peripheral solid tumors (PST), (2) identify the factors influencing ICU survival in PMBT patients. Two-center retrospective case-control study from March 1996 to May 2014. Primary central nervous system lymphomas were also included. Eighty-four patients with PMBT and 133 patients with PST were included. Acute infectious pneumonia was more frequent in PMBT than PST patients (77 vs. 36%, p
Published: 2018

12. New insight in spiral drawing analysis methods - Application to action tremor quantification

Author: Emmanuel Roze, André Pierre Legrand, Isabelle Rivals, Aliénor Richard, Marie Vidailhet, Elodie Hainque, Emmanuelle Apartis, Sabine Meunier, Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL), Neurophysiologie Respiratoire Expérimentale et Clinique (UMRS 1158), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Institut du Cerveau et de la Moëlle Epinière = Brain and Spine Institute (ICM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), CHU Pitié-Salpêtrière [AP-HP], Sons, Laboratoire de Mécanique et d'Acoustique [Marseille] (LMA ), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), Centre d'investigation clinique Neurosciences [CHU Pitié Salpêtrière] (CIC Neurosciences), Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)
Subjects: 0301 basic medicine, Adult, Male, Handwriting, Computer science, Essential Tremor, Hilbert–Huang transform, 03 medical and health sciences, Young Adult, 0302 clinical medicine, Physiology (medical), medicine, Humans, Diagnosis, Computer-Assisted, Spiral, Analysis method, Aged, Signal processing, Reproducibility, Essential tremor, business.industry, Numerical analysis, Reproducibility of Results, Pattern recognition, Middle Aged, medicine.disease, Action tremor, Sensory Systems, 030104 developmental biology, Neurology, Motor Skills, Computers, Handheld, Female, Neurology (clinical), Artificial intelligence, business, Spiral drawing analysis Essential tremor Unravelling Empirical mode decomposition, 030217 neurology & neurosurgery, [PHYS.PHYS.PHYS-DATA-AN]Physics [physics]/Physics [physics]/Data Analysis, Statistics and Probability [physics.data-an]
Abstract: International audience; Objective: Spiral drawing is one of the standard tests used to assess tremor severity for the clinical evaluation of medical treatments. Tremor severity is estimated through visual rating of the drawings by movement disorders experts. Different approaches based on the mathematical signal analysis of the recorded spiral drawings were proposed to replace this rater dependent estimate. The objective of the present study is to propose new numerical methods and to evaluate them in terms of agreement with visual rating and reproducibility. Methods: Series of spiral drawings of patients with essential tremor were visually rated by a board of experts. In addition to the usual velocity analysis, three new numerical methods were tested and compared, namely static and dynamic unraveling, and empirical mode decomposition. The reproducibility of both visual and numerical ratings was estimated, and their agreement was evaluated. Results: The statistical analysis demonstrated excellent agreement between visual and numerical ratings, and more reproducible results with numerical methods than with visual ratings. Conclusions: The velocity method and the new numerical methods are in good agreement. Among the latter, static and dynamic unravelling both display a smaller dispersion and are easier for automatic analysis. Significance: The reliable scores obtained through the proposed numerical methods allow considering that their implementation on a digitized tablet, be it connected with a computer or independent, provides an efficient automatic tool for tremor severity assessment.
Published: 2017
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13. Measuring Ventilatory Activity with Structured Light Plethysmography (SLP) Reduces Instrumental Observer Effect and Preserves Tidal Breathing Variability in Healthy and COPD

Author: Marie-Cécile Niérat, Bruno-Pierre Dubé, Claudia Llontop, Agnès Bellocq, Lila Layachi Ben Mohamed, Isabelle Rivals, Christian Straus, Thomas Similowski, Pierantonio Laveneziana, Neurophysiologie Respiratoire Expérimentale et Clinique, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de Pneumologie et Réanimation Médicale [CHU Pitié-Salpêtrière] (Département ' R3S '), CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre Hospitalier de l'Université de Montréal (CHUM), Université de Montréal (UdeM), Service des Explorations Fonctionnelles de la Respiration, de l'Exercice et de la Dyspnée - R3S', Pôle PRAGUES, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Equipe de Statistique Appliquée (UMRS 1158) (ESA), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique (UMRS 1158), HAL UPMC, Gestionnaire, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), and Service d'Explorations Fonctionnelles de la Respiration, de l’Exercice et de la Dyspnée [CHU Pitié-Salpêtrière]
Subjects: medicine.medical_specialty, genetic structures, [SDV.MHEP.PHY] Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Physiology, Copd patients, healthy, lcsh:Physiology, Structured light plethysmography, 03 medical and health sciences, 0302 clinical medicine, Breathing pattern, Physiology (medical), Tidal breathing, Internal medicine, [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Medicine, Plethysmograph, COPD, Mouthpiece, Original Research, lcsh:QP1-981, plethysmography, business.industry, instrumental/observer effect, Healthy subjects, medicine.disease, equipment and supplies, eye diseases, Surgery, 030228 respiratory system, Cardiology, sense organs, breathing variability, breathing pattern, business, 030217 neurology & neurosurgery
Abstract: International audience; The use of a mouthpiece to measure ventilatory flow with a pneumotachograph (PNT) introduces a major perturbation to breathing (" instrumental/observer effect ") and suffices to modify the respiratory behavior. Structured light plethysmography (SLP) is a non-contact method of assessment of breathing pattern during tidal breathing. Firstly, we validated the SLP measurements by comparing timing components of the ventilatory pattern obtained by SLP vs. PNT under the same condition; secondly, we compared SLP to SLP+PNT measurements of breathing pattern to evaluate the disruption of breathing pattern and breathing variability in healthy and COPD subjects. Measurements were taken during tidal breathing with SLP alone and SLP+PNT recording in 30 COPD and healthy subjects. Measurements included: respiratory frequency (Rf), inspiratory, expiratory, and total breath time/duration (TI, TE, and TT). Passing-Bablok regression analysis was used to evaluate the interchangeability of timing components of the ventilatory pattern (Rf, TI, TE, and TT) between measurements performed under the following experimental conditions: SLP vs. PNT, SLP+PNT vs. SLP, and SLP+PNT vs. PNT. The variability of different ventilatory variables was assessed through their coefficients of variation (CVs). In healthy: according to Passing-Bablok regression, Rf, TI, TE and TT were interchangeable between measurements obtained under the three experimental conditions (SLP vs. PNT, SLP+PNT vs. SLP, and SLP+PNT vs. PNT). All the CVs describing " traditional " ventilatory variables (Rf, TI, TE, TI/TE, and TI/TT) were significantly smaller in SLP+PNT condition. This was not the case for more " specific " SLP-derived variables. In COPD: according to Passing-Bablok regression, Rf, TI, TE, and TT were interchangeable between measurements obtained under SLP vs. PNT and SLP+PNT vs. PNT, whereas only Rf, TE, and TT were interchangeable between measurements obtained under SLP+PNT vs. SLP. However, Niérat et al. SLP and Breathing Variability in COPD most discrete variables were significantly different between the SLP and SLP+PNT conditions and CVs were significantly lower when COPD patients were assessed in the SLP+PNT condition. Measuring ventilatory activity with SLP preserves resting tidal breathing variability, reduces instrumental observer effect and avoids any disruptions in breathing pattern induced by the use of PNT-mouthpiece-nose-clip combination.
Published: 2017
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14. Ceramides and sphingomyelinases in senile plaques

Author: Luce Dauphinot, Maï Panchal, Olivier Laprévote, Massimo Masserini, Isabelle Rivals, Charles Duyckaerts, Adina N. Lazar, Delphine Dargère, Nicolas Auzeil, Sophie Ayciriex, Elisa Salvati, Mathieu Gaudin, Faculté de Pharmacie de Paris - Université Paris Descartes (UPD5 Pharmacie), Université Paris Descartes - Paris 5 (UPD5), Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), Max-Planck-Gesellschaft, Institut des Sciences Analytiques (ISA), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Lyon, Institut de Chimie des Substances Naturelles (ICSN), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Géosciences Marines (GM), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Equipe de Statistique Appliquée (UMRS 1158) (ESA), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique (UMRS 1158), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Laboratoire de Neurobiologie, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Chimie et Toxicologie Analytique et Cellulaire (EA 4463), Laboratoire de Neuropathologie Raymond Escourolle [CHU Pitié-Salpétriêre], Université Pierre et Marie Curie - Paris 6 (UPMC)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Panchal, M, Gaudin, M, Lazar, A, Salvati, E, Rivals, I, Ayciriex, S, Dauphinot, L, Dargère, D, Auzeil, N, Masserini, M, Laprévote, O, Duyckaerts, C, Université Paris Descartes - Faculté de Pharmacie de Paris ( UPD5 Pharmacie ), Université Paris Descartes - Paris 5 ( UPD5 ), Max Planck Institute for Cell Biology and Genetics, Max-Planck-Institut, Institut des Sciences Analytiques ( ISA ), École normale supérieure - Lyon ( ENS Lyon ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique ( CNRS ), Université Claude Bernard Lyon 1 ( UCBL ), ICNS - Institut de Chimie des Substances naturelles, Unité de recherche Géosciences Marines (Ifremer) ( GM ), Institut Français de Recherche pour l'Exploitation de la Mer ( IFREMER ), Equipe de Statistique Appliquée ( ESA ), ESPCI ParisTech, ESPCI ParisTech-Centre National de la Recherche Scientifique ( CNRS ), Laboratoire de Chimie et Toxicologie Analytique et Cellulaire ( EA 4463 ), Institut de Chimie des Substances Naturelles ( ICSN ), Centre National de la Recherche Scientifique ( CNRS ), Laboratoire de Neuropathologie Raymond Escourolle, Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Assistance publique - Hôpitaux de Paris (AP-HP)-CHU Pitié-Salpêtrière [APHP], Université Paris Descartes - Faculté de Pharmacie de Paris (UPD5 Pharmacie), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Unité de recherche Géosciences Marines (Ifremer) (GM), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique, CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Pierre et Marie Curie - Paris 6 (UPMC), Centre National de la Recherche Scientifique (CNRS)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-École normale supérieure - Lyon (ENS Lyon), Equipe de Statistique Appliquée (ESA), ESPCI ParisTech-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS), and Université Pierre et Marie Curie - Paris 6 (UPMC)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-CHU Pitié-Salpêtrière [APHP]
Subjects: Male, Amyloid beta-Peptide, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Plaque, Amyloid, Ceramide, [ SDV.BBM.BC ] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], 0302 clinical medicine, Senile plaques, [ SDV.BIBS ] Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], ComputingMilieux_MISCELLANEOUS, Aged, 80 and over, 0303 health sciences, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], Chemistry, Lipid, Middle Aged, Lipidome, BIO/10 - BIOCHIMICA, [SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], Sphingomyelin Phosphodiesterase, medicine.anatomical_structure, Neurology, Biochemistry, [ SDV.BBM.GTP ] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [ SDV.NEU.NB ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Female, Alzheimer's disease, Alzheimer disease, Sphingomyelin, Microdissection, Human, Electrospray ionization, Fibril, Ceramides, lcsh:RC321-571, 03 medical and health sciences, Apolipoproteins E, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Aβ peptide, Lipidomics, Neuropil, medicine, Humans, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry, Aged, 030304 developmental biology, Amyloid beta-Peptides, Senile plaque, Mass spectrometry, Lipidomic, Surface Plasmon Resonance, medicine.disease, Molecular biology, [ CHIM.ANAL ] Chemical Sciences/Analytical chemistry, Sphingomyelinase, Laser microdissection, 030217 neurology & neurosurgery, [ SDV.BBM.BS ] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], Chromatography, Liquid
Abstract: The senile plaque is a hallmark lesion of Alzheimer disease (AD). We compared, without a priori, the lipidome of the senile plaques and of the adjacent plaque-free neuropil. The analysis by liquid chromatography coupled with electrospray ionization mass spectrometry revealed that laser microdissected senile plaques were enriched in saturated ceramides Cer(d18:1/18:0) and Cer(d18:1/20:0) by 33 and 78% respectively with respect to the surrounding neuropil. This accumulation of ceramides was not explained by their affinity for Aβ deposits: no interaction between ceramide-liposomes and Aβ fibrils was observed in vitro by surface plasmon resonance and fluorescent ceramide-liposomes showed no affinity for the senile plaques in AD brain tissue. Accumulation of ceramides could be, at least partially, the result of a local production by acid and neutral sphingomyelinases that we found to be present in the corona of the senile plaques. © 2014 Elsevier Inc.
Published: 2014
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15. Structural and Molecular Alterations of Primary Afferent Fibres in the Spinal Dorsal Horn in Vincristine-Induced Neuropathy in Rat

Author: Sophie Dubacq, Sophie Pezet, Bernard Calvino, Karine Thibault, Saïd M'Dahoma, Isabelle Rivals, Laboratoire Plasticité du Cerveau Brain Plasticity (UMR 8249) (PdC), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Equipe de Statistique Appliquée (UMRS 1158) (ESA), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Institut de psychiatrie et neurosciences (U894 / UMS 1266), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Physique pour la médecine (UMR 8063, U1273), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Vincristine, Hot Temperature, Rats, Sprague-Dawley, 03 medical and health sciences, Cellular and Molecular Neuroscience, 0302 clinical medicine, Ganglia, Spinal, medicine, Animals, Premovement neuronal activity, Posterior Horn Cell, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 0303 health sciences, business.industry, Neuropeptides, General Medicine, Spinal cord, medicine.disease, Rats, 3. Good health, Posterior Horn Cells, Cytoskeletal Proteins, medicine.anatomical_structure, Allodynia, Peripheral neuropathy, Hyperalgesia, Touch, Neuropathic pain, Neuralgia, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], medicine.symptom, business, Neuroscience, 030217 neurology & neurosurgery, medicine.drug
Abstract: Vincristine is one of the most common anti-cancer drug therapies administered for the treatment of many types of cancer. Its dose-limiting side effect is the emergence of peripheral neuropathy, resulting in chronic neuropathic pain in many patients. This study sought to understand the mechanisms underlying the development of neuropathic pain by vincristine-induced neurotoxicity. We focused on signs of functional changes and revealed that deep layers of the spinal cord (III-IV) experience increased neuronal activity both in the absence of peripheral stimulation and, as a result of tactile mechanical stimulations. These laminae and superficial laminae I-II were also subject to structural changes as evidenced by an increase in immunoreactivity of Piccolo, a marker of active presynaptic elements. Further investigations performed, using DNA microarray technology, describe a large number of genes differentially expressed in dorsal root ganglions and in the spinal dorsal horn after vincristine treatment. Our study describes an important list of genes differentially regulated by vincristine treatment that will be useful for future studies and brings forward evidence for molecular and anatomical modifications of large diameter sensory neurons terminating in deep dorsal horn laminae, which could participate in the development of tactile allodynia.
Published: 2013
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16. A semi-automated computer tool for the analysis of retinal vessel diameter dynamics

Author: Michel Paques, José-Alain Sahel, O. Genevois, Pascale Massin, Isabelle Rivals, AméLie Collet, Guillaume Euvrard, Equipe de Statistique Appliquée (UMRS 1158) (ESA), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique (UMRS 1158), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), ANAGOS, CHU Rouen, Normandie Université (NU), Centre Hospitalier Lariboisi ère, Université Paris Diderot - Paris 7 (UPD7), Université Pierre et Marie Curie - Paris 6 (UPMC), Institut de la Vision, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Rivals, Isabelle, and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique
Subjects: Adult, Spectrum analyzer, Computer tools, [INFO.INFO-TS] Computer Science [cs]/Signal and Image Processing, Computer science, Video Recording, Hemodynamics, Health Informatics, Diagnostic Techniques, Ophthalmological, Pulse Amplitude, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, [INFO.INFO-TS]Computer Science [cs]/Signal and Image Processing, Heart Rate, Image Processing, Computer-Assisted, Humans, Computer vision, Retinal Vessel Analyser, [SPI.SIGNAL] Engineering Sciences [physics]/Signal and Image processing, Signal processing, business.industry, Dynamics (mechanics), Retinal Vessels, Signal Processing, Computer-Assisted, Retinal, Heart Beat Rate, [SDV.MHEP.CSC] Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, Computer Science Applications, Retinal vessel, chemistry, Pulse-amplitude modulation, Signal Processing, Linear Models, cardiovascular system, 030221 ophthalmology & optometry, Artificial intelligence, Artifacts, business, [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing, 030217 neurology & neurosurgery
Abstract: International audience; Retinal vessels are directly accessible to clinical observation. This has numerous potential interests for medical investigations. Using the Retinal Vessel Analyzer, a dedicated eye fundus camera enabling dynamic, video-rate recording of micrometric changes of the diameter of retinal vessels, we developed a semi-automated computer tool that extracts the heart beat rate and pulse amplitude values from the records. The extracted data enabled us to show that there is a decreasing relationship between heart beat rate and pulse amplitude of arteries and veins. Such an approach will facilitate the modeling of hemodynamic interactions in small vessels.
Published: 2013
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17. Desogestrel enhances ventilation in ondine patients: Animal data involving serotoninergic systems

Author: Fanny Joubert, Isabelle Rivals, Christian Straus, Emilienne Verkaeren, Alain Frugière, Laurence Bodineau, Anne-Sophie Perrin-Terrin, Marie-Noëlle Fiamma, Philippe J.P. Cardot, Thomas Similowski, Neurophysiologie Respiratoire Expérimentale et Clinique, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Hypoxie et Poumon : pneumopathologies fibrosantes, modulations ventilatoires et circulatoires (H&P), Université Paris 13 (UP13)-UFR SMBH, Equipe de Statistique Appliquée (UMRS 1158) (ESA), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Service de Pneumologie et Réanimation Médicale [CHU Pitié-Salpêtrière] (Département ' R3S '), CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service des Explorations Fonctionnelles de la Respiration, de l'Exercice et de la Dyspnée - R3S', Pôle PRAGUES, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique (UMRS 1158), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Service d'Explorations Fonctionnelles de la Respiration, de l’Exercice et de la Dyspnée [CHU Pitié-Salpêtrière], and HAL-UPMC, Gestionnaire
Subjects: Male, 0301 basic medicine, Ex vivo medullary-spinal cord preparations, Mice, 0302 clinical medicine, Medicine, Respiratory system, Medulla Oblongata, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Hypoventilation, Sleep Apnea, Central, 3. Good health, Spinal Cord, Cardiology, Breathing, Female, medicine.symptom, Serotonergic Neurons, medicine.drug, Adult, medicine.medical_specialty, Central congenital hypoventilation syndrome, Serotonergic, Young Adult, 03 medical and health sciences, Cellular and Molecular Neuroscience, Animal data, Organ Culture Techniques, Desogestrel, Internal medicine, In vivo, Animals, Humans, Plethysmograph, GABA-A Receptor Agonists, Etonogestrel, Pharmacology, Dose-Response Relationship, Drug, Raphe, business.industry, Progestin, 030104 developmental biology, Endocrinology, Animals, Newborn, Pulmonary Ventilation, business, 030217 neurology & neurosurgery, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: International audience; Central congenital hypoventilation syndrome (CCHS) is a neurorespiratory disease characterized by life-threatening sleep-related hypoventilation involving an alteration of CO2/H+ chemosensitivity. Incidental findings have suggested that desogestrel may allow recovery of the ventilatory response to CO2. The effects of desogestrel on resting ventilation have not been reported. This study was designed to test the hypothesis that desogestrel strengthens baseline ventilation by analyzing the ventilation of CCHS patients. Rodent models were used in order to determine the mechanisms involved. Ventilation in CCHS patients was measured with a pneumotachometer. In mice, ventilatory neural activity was recorded from ex vivo medullary-spinal cord preparations, ventilation was measured by plethysmography and c-fos expression was studied in medullary respiratory nuclei. Desogestrel increased baseline respiratory frequency of CCHS patients leading to a decrease in their PETCO2. In medullary spinal-cord preparations or in vivo mice, the metabolite of desogestrel, etonogestrel, induced an increase in respiratory frequency that necessitated the functioning of serotoninergic systems, and modulated GABAA and NMDA ventilatory regulations. c-FOS analysis showed the involvement of medullary respiratory groups of cell including serotoninergic neurons of the raphe pallidus and raphe obscurus nuclei that seem to play a key role. Thus, desogestrel may improve resting ventilation in CCHS patients by a stimulant effect on baseline respiratory frequency. Our data open up clinical perspectives based on the combination of this progestin with serotoninergic drugs to enhance ventilation in CCHS patients.
Published: 2016
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18. Relieving dyspnoea by non-invasive ventilation decreases pain thresholds in amyotrophic lateral sclerosis

Author: Louis Laviolette, Thomas Similowski, Jésus Gonzalez-Bermejo, Capucine Morélot-Panzini, Marjolaine Georges, Isabelle Rivals, Laurence Dangers, Neurophysiologie Respiratoire Expérimentale et Clinique (UMRS 1158), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Institut Universitaire de Cardiologie et de Pneumologie de Québec (IUCPQ), Université Laval [Québec] (ULaval), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL), and MORELOT-PANZINI, Capucine
Subjects: Pulmonary and Respiratory Medicine, Adult, Male, Pain Threshold, Visual analogue scale, [SDV]Life Sciences [q-bio], Deltoid curve, 03 medical and health sciences, 0302 clinical medicine, Sensation, Medicine, Humans, Pain Management, In patient, Perception of Asthma/Breathlessness, Respiratory system, Amyotrophic lateral sclerosis, Aged, Pain Measurement, Noninvasive Ventilation, business.industry, Amyotrophic Lateral Sclerosis, Non invasive ventilation, Middle Aged, medicine.disease, [SDV] Life Sciences [q-bio], Dyspnea, 030228 respiratory system, Respiratory failure, Anesthesia, Breathing, Female, France, business, 030217 neurology & neurosurgery
Abstract: International audience; Background: Dyspnoea is a threatening sensation of respiratory discomfort that presents many similarities with pain. Experimental dyspnoea in healthy subjects induces analgesia. This 'dyspnoea-pain counter-irritation' could, in reverse, imply that relieving dyspnoea in patients with chronic respiratory diseases would lower their pain thresholds.Methods: We first determined pressure pain thresholds in 25 healthy volunteers (22-31 years; 13 men; handheld algometer), during unloaded breathing (BASELINE) and during inspiratory threshold loading (ITL). Two levels of loading were used, adjusted to induce dyspnoea self-rated at 60% or 80% of a 10 cm visual analogue scale (ITL6 and ITL8). 18 patients with chronic respiratory failure due to amyotrophic lateral sclerosis (ALS) were then studied during unassisted breathing and after 30 and 60 min of non-invasive ventilation-NIV30 and NIV60-(same dyspnoea evaluation).Results: In healthy volunteers, pressure pain thresholds increased significantly in the deltoid during ITL6 (p
Published: 2016
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19. Modifications of the endosomal compartment in peripheral blood mononuclear cells and fibroblasts from Alzheimer’s disease patients

Author: Charles Duyckaerts, Malaplate-Armand C, Olivier Jl, Marie-Claude Potier, Isabelle Rivals, Marie Sarazin, Michel Bottlaender, Lorraine Hamelin, Hélène Corne, Julien Lagarde, Luce Dauphinot, Bruno Dubois, Kunie Ando, Guillaume Dorothée, G. Fontaine, Cossec Jc, Fabian Corlier, Institut du Cerveau et de la Moëlle Epinière = Brain and Spine Institute (ICM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-CHU Pitié-Salpêtrière [APHP], Equipe de Statistique Appliquée, Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI ParisTech), Institut de psychiatrie et neurosciences (U894 / UMS 1266), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Pathologies biliaires, fibrose et cancer du foie (Inserm UMR_S 938), Centre de Recherche Saint-Antoine (CR Saint-Antoine), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Saint-Antoine [APHP], Unité de Recherches Animal et Fonctionnalités des Produits Animaux (URAFPA), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), Laboratoire de Biochimie et Biologie Moléculaire, Institut de la Mémoire et de la Maladie d'Alzheimer [Paris] (IM2A), Université Pierre et Marie Curie - Paris 6 (UPMC), Institut d'Imagerie BioMédicale (I2BM), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Laboratoire de Neuropathologie Raymond Escourolle, Université Pierre et Marie Curie - Paris 6 (UPMC)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-CHU Pitié-Salpêtrière [APHP], Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Equipe de Statistique Appliquée (UMRS 1158) (ESA), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique (UMRS 1158), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Pathologies biliaires, fibrose et cancer du foie, Centre de Recherche Saint-Antoine (UMRS893), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Institut de la Mémoire et de la Maladie d'Alzheimer [CHU Pitié-Salpétriêre] (IM2A), Université Pierre et Marie Curie - Paris 6 (UPMC)-CHU Pitié-Salpêtrière [AP-HP], Laboratoire de Neuropathologie Raymond Escourolle [CHU Pitié-Salpétriêre], HAL-UPMC, Gestionnaire, Centre de Psychiatrie et Neurosciences (U894), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique, CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Centre de Recherche Saint-Antoine (UMRS893), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Institut du Cerveau et de la Moëlle Epinière = Brain and Spine Institute ( ICM ), Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -CHU Pitié-Salpêtrière [APHP], Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris ( ESPCI ParisTech ), Centre de Psychiatrie et Neurosciences ( CPN - U894 ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université Paris Descartes - Paris 5 ( UPD5 ), Pathologies biliaires, fibrose et cancer du foie ( Inserm UMR_S 938 ), Centre de Recherche Saint-Antoine ( CR Saint-Antoine ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université Pierre et Marie Curie - Paris 6 ( UPMC ) -CHU Saint-Antoine [APHP], Unité de Recherches Animal et Fonctionnalités des Produits Animaux ( URAFPA ), Université de Lorraine ( UL ) -Institut National de la Recherche Agronomique ( INRA ), Institut de la Mémoire et de la Maladie d'Alzheimer [Paris] ( IM2A ), Université Pierre et Marie Curie - Paris 6 ( UPMC ), Institut d'Imagerie BioMédicale ( I2BM ), Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Saclay, and CHU Pitié-Salpêtrière [APHP]-Assistance publique - Hôpitaux de Paris (AP-HP)-Université Pierre et Marie Curie - Paris 6 ( UPMC )
Subjects: Apolipoprotein E, Male, Pathology, [ SDV.MHEP.PSM ] Life Sciences [q-bio]/Human health and pathology/Psychiatrics and mental health, [SDV.MHEP.PSM] Life Sciences [q-bio]/Human health and pathology/Psychiatrics and mental health, Amyloid beta-Peptides -- cerebrospinal fluid, 0302 clinical medicine, Cerebrospinal fluid, Apolipoproteins E -- genetics, Biomarkers -- blood -- cerebrospinal fluid -- metabolism, Aged, 80 and over, 0303 health sciences, Microscopy, Confocal, Sciences bio-médicales et agricoles, Middle Aged, 3. Good health, Psychiatry and Mental health, Endosomes -- pathology, Biomarker (medicine), Original Article, Female, medicine.medical_specialty, Amyloid, Fibroblasts -- pathology, Endosome, Immunocytochemistry, Neuroimaging, tau Proteins, Endosomes, Peripheral blood mononuclear cell, Cognitive Dysfunction -- blood -- diagnosis -- physiopathology, 03 medical and health sciences, Cellular and Molecular Neuroscience, Apolipoproteins E, Alzheimer Disease, medicine, Dementia, Humans, Cognitive Dysfunction, Biological Psychiatry, 030304 developmental biology, Aged, Leukocytes, Mononuclear -- physiology, Amyloid beta-Peptides, business.industry, tau Proteins -- cerebrospinal fluid, Médecine pathologie humaine, Fibroblasts, medicine.disease, Case-Control Studies, Positron-Emission Tomography, [SDV.MHEP.PSM]Life Sciences [q-bio]/Human health and pathology/Psychiatrics and mental health, Leukocytes, Mononuclear, Alzheimer Disease -- blood -- diagnosis -- pathology, business, 030217 neurology & neurosurgery, Biomarkers
Abstract: Identification of blood-based biomarkers of Alzheimer's disease (AD) remains a challenge. Neuropathological studies have identified enlarged endosomes in post-mortem brains as the earliest cellular change associated to AD. Here the presence of enlarged endosomes was investigated in peripheral blood mononuclear cells from 48 biologically defined AD patients (25 with mild cognitive impairment and 23 with dementia (AD-D)), and 23 age-matched healthy controls using immunocytochemistry and confocal microscopy. The volume and number of endosomes were not significantly different between AD and controls. However, the percentage of cells containing enlarged endosomes was significantly higher in the AD-D group as compared with controls. Furthermore, endosomal volumes significantly correlated to [C(11)]PiB cortical index measured by positron emission tomography in the AD group, independently of the APOE genotype, but not to the levels of amyloid-beta, tau and phosphorylated tau measured in the cerebrospinal fluid. Importantly, we confirmed the presence of enlarged endosomes in fibroblasts from six unrelated AD-D patients as compared with five cognitively normal controls. This study is the first, to our knowledge, to report morphological alterations of the endosomal compartment in peripheral cells from AD patients correlated to amyloid load that will now be evaluated as a possible biomarker., info:eu-repo/semantics/published
Published: 2015
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20. Trisomy for synaptojanin1 in Down syndrome is functionally linked to the enlargement of early endosomes

Author: Yann Grattau, Gilbert Di Paolo, Clémentine Ripoll, Charles Duyckaerts, Jack-Christophe Cossec, Isabelle Rivals, Samantha Stora, Fabrice de Chaumont, Diego E. Berman, Joris A. Veltman, Magalie Lecourtois, Jean M. Delabar, Marie-Claude Potier, Jérémie Lavaur, Stylianos E. Antonarakis, Jean-Christophe Olivo-Marin, Clotilde Mircher, Alexander Hoischen, Centre de Recherche de l'Institut du Cerveau et de la Moelle épinière (CRICM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Columbia University Medical Center (CUMC), Columbia University [New York], Equipe de Statistique Appliquée (UMRS 1158) (ESA), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Radboud University Medical Center [Nijmegen], Institut Jérôme Lejeune, Unité de Biologie Fonctionnelle et Adaptative (BFA (UMR_8251 / U1133)), Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Analyse d'Images Quantitative (AIQ), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Génétique médicale et fonctionnelle du cancer et des maladies neuropsychiatriques, Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), University of Geneva [Switzerland], This work was supported by the Agence Nationale de la Recherche (ChoAD to M.-C.P. and C.D.), the European Communities (AnEUploidy to M.-C.P., A.H., Y.G., S.E.A., J.A.V. and J.M.D.), the Fondation ICM and the Fondation Thierry et Annick Desmaret-Institut de France to M.C.P. and C.D., the National Institutes of Health (R01 HD055457 to G.D.P.), and the Foundation Jerome Lejeune to G.D.P., Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC), Equipe de Statistique Appliquée (ESA), ESPCI ParisTech, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique (UMRS 1158), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), and Université de Genève = University of Geneva (UNIGE)
Subjects: Nerve Tissue Proteins/genetics/metabolism, Cell type, down syndrome, Chromosomes, Human, Pair 21, Endosome, Transgene, Endocytic cycle, neurons, Nerve Tissue Proteins, Mice, Transgenic, Trisomy, Biology, Endocytosis, Cell Line, protein overexpression, Mice, 03 medical and health sciences, 0302 clinical medicine, RNA interference, Chromosomes, Human, Pair 21/enzymology/genetics, fibroblasts, Endosomes/chemistry/metabolism, Genetics, Amyloid precursor protein, Animals, Humans, Gene silencing, ddc:576.5, Molecular Biology, Genetics (clinical), 030304 developmental biology, 0303 health sciences, trisomy, Phosphoric Monoester Hydrolases/genetics/metabolism, Chromosome Mapping, Articles, General Medicine, Phosphoric Monoester Hydrolases, Cell biology, endosomes, Down Syndrome/enzymology/genetics, Immunology, biology.protein, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Genetics and epigenetic pathways of disease Genomic disorders and inherited multi-system disorders [NCMLS 6], 030217 neurology & neurosurgery
Abstract: Item does not contain fulltext Enlarged early endosomes have been observed in neurons and fibroblasts in Down syndrome (DS). These endosome abnormalities have been implicated in the early development of Alzheimer's disease (AD) pathology in these subjects. Here, we show the presence of enlarged endosomes in blood mononuclear cells and lymphoblastoid cell lines (LCLs) from individuals with DS using immunofluorescence and confocal microscopy. Genotype-phenotype correlations in LCLs carrying partial trisomies 21 revealed that triplication of a 2.56 Mb locus in 21q22.11 is associated with the endosomal abnormalities. This locus contains the gene encoding the phosphoinositide phosphatase synaptojanin 1 (SYNJ1), a key regulator of the signalling phospholipid phosphatidylinositol-4,5-biphosphate that has been shown to regulate clathrin-mediated endocytosis. We found that SYNJ1 transcripts are increased in LCLs from individuals with DS and that overexpression of SYNJ1 in a neuroblastoma cell line as well as in transgenic mice leads to enlarged endosomes. Moreover, the proportion of enlarged endosomes in fibroblasts from an individual with DS was reduced after silencing SYNJ1 expression with RNA interference. In LCLs carrying amyloid precursor protein (APP) microduplications causing autosomal dominant early-onset AD, enlarged endosomes were absent, suggesting that APP overexpression alone is not involved in the modification of early endosomes in this cell type. These findings provide new insights into the contribution of SYNJ1 overexpression to the endosomal changes observed in DS and suggest an attractive new target for rescuing endocytic dysfunction and lipid metabolism in DS and in AD.
Published: 2012
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21. Functional genomics and molecular networks Gene expression regulations in complex diseases: Down syndrome as a case study

Author: Isabelle Rivals, Marie-Claude Potier, Centre de Recherche de l'Institut du Cerveau et de la Moelle épinière (CRICM), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC), Equipe de Statistique Appliquée (UMRS 1158) (ESA), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), N. Le Novère, Rivals, Isabelle, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique (UMRS 1158)
Subjects: False discovery rate, differentially expressed genes, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, statistical tests, Biology, Genome, Transcriptome, 03 medical and health sciences, 0302 clinical medicine, Gene expression, Gene, Down syndrom, 030304 developmental biology, [INFO.INFO-BI] Computer Science [cs]/Bioinformatics [q-bio.QM], Genetics, 0303 health sciences, [STAT.ME] Statistics [stat]/Methodology [stat.ME], [SDV.BIBS] Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], [SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, DNA microarray, Phenotype, [SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], [INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM], Functional genomics, [STAT.ME]Statistics [stat]/Methodology [stat.ME], 030217 neurology & neurosurgery
Abstract: International audience; The goal of functional genomics is to understand the relationship be-tween whole genomes and phenotypes through a dynamic approach. It requires high throughput technologies such as microarrays and data analysis. The power of this approach allowed to study complex biological functions as well as diseases. In this chapter, we introduce functional genomics and describe the statistical methods that are used to find differentially expressed genes. We analyze a large num-ber of data sets produced on a complex disease, namely Down syn-drome, in different models. We show that, whatever the model, genes that are in three copies are globally overexpressed. However, we failed to identify a set of two-copy genes that would be dysregu-lated in all studies. It either suggests that studies are incomplete, or that this set of genes does not exist and that overexpression of the three-copy genes impacts on the whole transcriptome in a "stochas-tic" way.
Published: 2012

22. Purkinje Cell Maturation Participates in the Control of Oligodendrocyte Differentiation: Role of Sonic Hedgehog and Vitronectin

Author: Rosine Wehrlé, Yolande Lemaigre-Dubreuil, Isabelle Dusart, Lamia Bouslama-Oueghlani, Constantino Sotelo, Isabelle Rivals, Mohamed Doulazmi, Fanny Jaudon, Xiao Ru Chen, Neurobiologie des processus adaptatifs (NPA), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Equipe de Statistique Appliquée (UMRS 1158) (ESA), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique (UMRS 1158)
Subjects: Cerebellum, organotypic culture, Mouse, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Cellular differentiation, Purkinje cell, lcsh:Medicine, OPCs, Mice, Purkinje Cells, 0302 clinical medicine, Neurobiology of Disease and Regeneration, Sonic hedgehog, lcsh:Science, 0303 health sciences, Multidisciplinary, biology, Cell Differentiation, Animal Models, Hedgehog signaling pathway, 3. Good health, Cell biology, Axon Guidance, Up-Regulation, Oligodendroglia, medicine.anatomical_structure, Cerebellar cortex, Vitronectin, Research Article, Down-Regulation, Mice, Transgenic, Signaling Pathways, 03 medical and health sciences, Model Organisms, Developmental Neuroscience, Neuroglial Development, medicine, Animals, Hedgehog Proteins, development, Biology, 030304 developmental biology, Cell Proliferation, lcsh:R, Oligodendrocyte differentiation, oligodendrocyte differentiation, Animals, Newborn, Cellular Neuroscience, Immunology, biology.protein, lcsh:Q, Neural Circuit Formation, Molecular Neuroscience, 030217 neurology & neurosurgery, Neuroscience
Abstract: Oligodendrocyte differentiation is temporally regulated during development by multiple factors. Here, we investigated whether the timing of oligodendrocyte differentiation might be controlled by neuronal differentiation in cerebellar organotypic cultures. In these cultures, the slices taken from newborn mice show very few oligodendrocytes during the first week of culture (immature slices) whereas their number increases importantly during the second week (mature slices). First, we showed that mature cerebellar slices or their conditioned media stimulated oligodendrocyte differentiation in immature slices thus demonstrating the existence of diffusible factors controlling oligodendrocyte differentiation. Using conditioned media from different models of slice culture in which the number of Purkinje cells varies drastically, we showed that the effects of these differentiating factors were proportional to the number of Purkinje cells. To identify these diffusible factors, we first performed a transcriptome analysis with an Affymetrix array for cerebellar cortex and then real-time quantitative PCR on mRNAs extracted from fluorescent flow cytometry sorted (FACS) Purkinje cells of L7-GFP transgenic mice at different ages. These analyses revealed that during postnatal maturation, Purkinje cells down-regulate Sonic Hedgehog and up-regulate vitronectin. Then, we showed that Sonic Hedgehog stimulates the proliferation of oligodendrocyte precursor cells and inhibits their differentiation. In contrast, vitronectin stimulates oligodendrocyte differentiation, whereas its inhibition with blocking antibodies abolishes the conditioned media effects. Altogether, these results suggest that Purkinje cells participate in controlling the timing of oligodendrocyte differentiation in the cerebellum through the developmentally regulated expression of diffusible molecules such as Sonic Hedgehog and vitronectin. © 2012 Bouslama-Oueghlani et al., This work was supported by the Centre National de la Recherche Scientifique (CNRS): ATIP, University Pierre et Marie Curie (UPMC), the Institut National Scientifique pour la Recherche Médicale (INSERM), Association pour la Recherche sur le Cancer (ARC, contract 3532), RGN (Reseau Genopole National, microarray subvention) and the Agence National pour le Recherche (ANR, ANR-07-NEURO-043-01).
Published: 2012
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23. Axonal Targeting of the 5-HT1B Serotonin Receptor Relies on Structure-Specific Constitutive Activation

Author: Damien Carrel, Isabelle Rivals, Christophe Leterrier, Michèle Darmon, Anne Simon, Michel Hamon, Marc Biard, M. B. Emerit, Zsolt Lenkei, Leterrier, Christophe, Laboratoire Plasticité du Cerveau Brain Plasticity (UMR 8249) (PdC), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Neurobiologie, Institut de psychiatrie et neurosciences (U894 / UMS 1266), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Equipe de Statistique Appliquée (UMRS 1158) (ESA), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique (UMRS 1158), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Neurobiologie des Canaux Ioniques, Université de la Méditerranée - Aix-Marseille 2-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Unité de Neuropsychopharmacologie [CHU Pitié-Salpétriêre], Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Neurobiologie et diversité cellulaire (NDC), Centre de Psychiatrie et Neurosciences (U894), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de la Méditerranée - Aix-Marseille 2, Neuropsychopharmacologie, and Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)
Subjects: p11, Swine, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Hippocampus, Biochemistry, Cell membrane, 0302 clinical medicine, Structural Biology, Chlorocebus aethiops, Tumor Cells, Cultured, Axon, Receptor, Cells, Cultured, Neurons, axon, 0303 health sciences, Endocytosis, Transport protein, Cell biology, Protein Transport, medicine.anatomical_structure, COS Cells, Receptor, Serotonin, 5-HT1A, Receptor, Serotonin, 5-HT1B, polarized distribu, Intracellular, Molecular Sequence Data, Biology, Structure-Activity Relationship, 03 medical and health sciences, tion, Genetics, medicine, Animals, Humans, Amino Acid Sequence, Molecular Biology, constitutive activity, 5-HT receptor, targeting, 030304 developmental biology, G protein-coupled receptor, third intracellular loop, Cell Membrane, [SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Cell Biology, Axons, Protein Structure, Tertiary, Rats, 7-transmembrane receptor, LLC-PK1 Cells, 030217 neurology & neurosurgery, HeLa Cells
Abstract: International audience; . By analogy to other axonal proteins, transcytotic delivery following spontaneous endocytosis from the somatoden-dritic membrane is expected to be essential for polarized distribution of axonal G-protein coupled receptors (GPCRs). However, possible contribution from constitu-tive activation, which may also result in constitutive GPCR endocytosis, is poorly known. Using two closely related but differentially distributed serotonin receptors, here we demonstrate higher constitutive activation and spontaneous endocytosis for the axonal 5-HT 1B R, as compared to the somatodendritic 5-HT 1A R, both in non-neuronal cells and neurons. Activation-dependent consti-tutive endocytosis is crucial for axonal targeting, because inverse-agonist treatment, which prevents constitutive activation, leads to atypical accumulation of newly synthesized 5-HT 1B Rs on the somatodendritic plasma membrane. Using receptor chimeras composed of different domains from 5-HT 1A R and 5-HT 1B R, we show that the complete third intracellular loop of 5-HT 1B R is necessary and sufficient for constitutive activation and efficient axonal targeting, both sensitive to inverse-agonist treatment. These results suggest that activation and targeting of 5-HT 1B Rs are intimately interconnected in neurons.
Published: 2011
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24. Gene expression signature of cerebellar hypoplasia in a mouse model of Down syndrome during postnatal development

Author: Isabelle Rivals, Luce Dauphinot, Randal X. Moldrich, Julien Laffaire, Yann Herault, Tania Vitalis, Fabien Pasteau, Jean Rossier, Ralph Sinkus, Rosine Wehrlé, Benoit Larrat, Isabelle Dusart, Marie-Claude Potier, Laboratoire de Neurobiologie, Centre National de la Recherche Scientifique (CNRS)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), Centre de Recherche de l'Institut du Cerveau et de la Moelle épinière (CRICM), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC), Neurobiologie et diversité cellulaire (NDC), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Equipe de Statistique Appliquée (UMRS 1158) (ESA), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Neurobiologie des processus adaptatifs (NPA), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Ondes et Acoustique (UMR 7587) (LOA), Université Paris Diderot - Paris 7 (UPD7)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Quennsland Brain Institute, University of Queensland [Brisbane], Immunologie et Embryologie Moléculaires (IEM), Université d'Orléans (UO)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Ondes et Acoustique (LOA), Immunologie et embryologie moléculaires (IEM), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique (UMRS 1158), and Rivals, Isabelle
Subjects: Male, differentially expressed genes, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Aneuploidy, Trisomy, Bioinformatics, MESH: Animals, Newborn, Transcriptome, Mice, 0302 clinical medicine, transcriptome analysis, Cerebellum, MESH: Gene Expression Regulation, Developmental, MESH: Animals, Down syndrom, [INFO.INFO-BI] Computer Science [cs]/Bioinformatics [q-bio.QM], Oligonucleotide Array Sequence Analysis, Genetics, 0303 health sciences, Dosage compensation, [SDV.BIBS] Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], Gene Expression Regulation, Developmental, [SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], MESH: Trisomy, Research Article, Biotechnology, Down syndrome, lcsh:QH426-470, lcsh:Biotechnology, Biology, Gene dosage, Chromosomes, 03 medical and health sciences, MESH: Gene Expression Profiling, lcsh:TP248.13-248.65, MESH: Analysis of Variance, medicine, Animals, MESH: Mice, 030304 developmental biology, Analysis of Variance, gene dosage effect, Gene Expression Profiling, [SCCO.NEUR]Cognitive science/Neuroscience, [SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, DNA microarray, MESH: Down Syndrome, medicine.disease, MESH: Male, MESH: Cerebellum, Gene expression profiling, Disease Models, Animal, lcsh:Genetics, Animals, Newborn, MESH: Oligonucleotide Array Sequence Analysis, MESH: Chromosomes, Down Syndrome, MESH: Disease Models, Animal, [INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM], Chromosome 21, 030217 neurology & neurosurgery
Abstract: Background Down syndrome is a chromosomal disorder caused by the presence of three copies of chromosome 21. The mechanisms by which this aneuploidy produces the complex and variable phenotype observed in people with Down syndrome are still under discussion. Recent studies have demonstrated an increased transcript level of the three-copy genes with some dosage compensation or amplification for a subset of them. The impact of this gene dosage effect on the whole transcriptome is still debated and longitudinal studies assessing the variability among samples, tissues and developmental stages are needed. Results We thus designed a large scale gene expression study in mice (the Ts1Cje Down syndrome mouse model) in which we could measure the effects of trisomy 21 on a large number of samples (74 in total) in a tissue that is affected in Down syndrome (the cerebellum) and where we could quantify the defect during postnatal development in order to correlate gene expression changes to the phenotype observed. Statistical analysis of microarray data revealed a major gene dosage effect: for the three-copy genes as well as for a 2 Mb segment from mouse chromosome 12 that we show for the first time as being deleted in the Ts1Cje mice. This gene dosage effect impacts moderately on the expression of euploid genes (2.4 to 7.5% differentially expressed). Only 13 genes were significantly dysregulated in Ts1Cje mice at all four postnatal development stages studied from birth to 10 days after birth, and among them are 6 three-copy genes. The decrease in granule cell proliferation demonstrated in newborn Ts1Cje cerebellum was correlated with a major gene dosage effect on the transcriptome in dissected cerebellar external granule cell layer. Conclusion High throughput gene expression analysis in the cerebellum of a large number of samples of Ts1Cje and euploid mice has revealed a prevailing gene dosage effect on triplicated genes. Moreover using an enriched cell population that is thought responsible for the cerebellar hypoplasia in Down syndrome, a global destabilization of gene expression was not detected. Altogether these results strongly suggest that the three-copy genes are directly responsible for the phenotype present in cerebellum. We provide here a short list of candidate genes.
Published: 2009
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25. Classification of human chromosome 21 gene-expression variations in down syndrome: Impact on disease phenotypes

Author: Henri Bléhaut, L. Personnaz, Jean M. Delabar, G. Golfier, E. Aït Yahya-Graison, Luce Dauphinot, Jean Rossier, Marguerite Prieur, Isabelle Rivals, M.-C. Potier, Nicole Créau, Julie Aubert, Stéphane Robin, UMR7637, Centre National de la Recherche Scientifique (CNRS), Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI ParisTech), Université Paris Diderot - Paris 7 (UPD7), Mathématiques et Informatique Appliquées (MIA-Paris), AgroParisTech-Institut National de la Recherche Agronomique (INRA), CHU Necker - Enfants Malades [AP-HP], Institut Jérôme Lejeune, Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL), and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)
Subjects: Male, Down syndrome, Transcription, Genetic, Chromosomes, Human, Pair 21, [SDV]Life Sciences [q-bio], Molecular Sequence Data, Aneuploidy, Gene Expression, Biology, PHENOTYPE, Article, 03 medical and health sciences, Chromosome 15, 0302 clinical medicine, HUMAN CHROMOSOME 21, DOWN SYNDROME, medicine, Genetics, Humans, Genetics(clinical), Gene, Genetics (clinical), 030304 developmental biology, Oligonucleotide Array Sequence Analysis, GENE-EXPRESSION, 0303 health sciences, Base Sequence, Microarray analysis techniques, Genetic Variation, medicine.disease, Phenotype, Molecular biology, GENE, 3. Good health, Female, Trisomy, Chromosome 21, 030217 neurology & neurosurgery
Abstract: Down syndrome caused by chromosome 21 trisomy is the most common genetic cause of mental retardation in humans. Disruption of the phenotype is thought to be the result of gene-dosage imbalance. Variations in chromosome 21 gene expression in Down syndrome were analyzed in lymphoblastoid cells derived from patients and control individuals. Of the 359 genes and predictions displayed on a specifically designed high-content chromosome 21 microarray, one-third were expressed in lymphoblastoid cells. We performed a mixed-model analysis of variance to find genes that are differentially expressed in Down syndrome independent of sex and interindividual variations. In addition, we identified genes with variations between Down syndrome and control samples that were significantly different from the gene-dosage effect (1.5). Microarray data were validated by quantitative polymerase chain reaction. We found that 29% of the expressed chromosome 21 transcripts are overexpressed in Down syndrome and correspond to either genes or open reading frames. Among these, 22% are increased proportional to the gene-dosage effect, and 7% are amplified. The other 71% of expressed sequences are either compensated (56%, with a large proportion of predicted genes and antisense transcripts) or highly variable among individuals (15%). Thus, most of the chromosome 21 transcripts are compensated for the gene-dosage effect. Overexpressed genes are likely to be involved in the Down syndrome phenotype, in contrast to the compensated genes. Highly variable genes could account for phenotypic variations observed in patients. Finally, we show that alternative transcripts belonging to the same gene are similarly regulated in Down syndrome but sense and antisense transcripts are not.
Published: 2007
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26. Enrichment or depletion of a GO category within a class of genes: which test?

Author: Marie-Claude Potier, Léon Personnaz, Lieng Taing, Isabelle Rivals, Equipe de Statistique Appliquée (UMRS 1158) (ESA), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Laboratoire de Neurobiologie et de Diversité Cellulaire, and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Statistics and Probability, Class (set theory), Fisher's exact test, Information Storage and Retrieval, Binomial test, Biochemistry, 03 medical and health sciences, symbols.namesake, 0302 clinical medicine, GO category, Statistics, Null distribution, hypergeometric test, Databases, Protein, Molecular Biology, Equivalence (measure theory), 030304 developmental biology, Statistical hypothesis testing, Mathematics, Oligonucleotide Array Sequence Analysis, 0303 health sciences, Gene Expression Profiling, Proteins, p-value, [SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], Hypergeometric distribution, Computer Science Applications, Computational Mathematics, Exact test, Computational Theory and Mathematics, Data Interpretation, Statistical, Multigene Family, symbols, Database Management Systems, [INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM], [STAT.ME]Statistics [stat]/Methodology [stat.ME], 030217 neurology & neurosurgery
Abstract: Motivation: A number of available program packages determine the significant enrichments and/or depletions of GO categories among a class of genes of interest. Whereas a correct formulation of the problem leads to a single exact null distribution, these GO tools use a large variety of statistical tests whose denominations often do not clarify the underlying P-value computations. Summary: We review the different formulations of the problem and the tests they lead to: the binomial, χ2, equality of two probabilities, Fisher's exact and hypergeometric tests. We clarify the relationships existing between these tests, in particular the equivalence between the hypergeometric test and Fisher's exact test. We recall that the other tests are valid only for large samples, the test of equality of two probabilities and the χ2-test being equivalent. We discuss the appropriateness of one- and two-sided P-values, as well as some discreteness and conservatism issues. Contact: isabelle.rivals@espci.fr Supplementary information: Supplementary data are available at Bioinformatics online.
Published: 2007
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27. Detecting fluorescent protein expression and co-localisation on single secretory vesicles with linear spectral unmixing

Author: Isabelle Rivals, Marine Vernet, Christian Giaume, Fabien Nadrigny, Annette Koulakoff, Léon Personnaz, Frank Kirchhoff, Myriam M. Chaumeil, Petra G. Hirrlinger, Nicole Ropert, Martin Oheim, Myriam Allioux, Neurophysiologie et nouvelles microscopies (NNM (UM 82)), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Descartes - Paris 5 (UPD5), Equipe de Statistique Appliquée (UMRS 1158) (ESA), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique, and Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)
Subjects: Yellow fluorescent protein, medicine.medical_specialty, Vesicle-Associated Membrane Protein 2, [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph], Green Fluorescent Proteins, Biophysics, Green fluorescent protein, Mice, 03 medical and health sciences, 0302 clinical medicine, Bacterial Proteins, medicine, Fluorescence microscope, Animals, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 0303 health sciences, biology, Secretory Vesicles, Spectrum Analysis, General Medicine, Hydrogen-Ion Concentration, Fluorescence, Molecular biology, Spectral imaging, Luminescent Proteins, Autofluorescence, Förster resonance energy transfer, Microscopy, Fluorescence, Membrane protein, Astrocytes, biology.protein, 030217 neurology & neurosurgery
Abstract: Many questions in cell biology and biophysics involve the quantitation of co-localisation and the interaction of proteins tagged with different fluorophores. However, the incomplete separation of the different colour channels due to the presence of autofluorescence, along with cross-excitation and emission "bleed-through" of one colour channel into the other, all combine to render the interpretation of multi-band images ambiguous. Here we introduce a new live-cell epifluorescence spectral imaging and linear unmixing technique for classifying resolution-limited point objects containing multiple fluorophores. We demonstrate the performance of our technique by detecting, at the single-vesicle level, the co-expression of the vesicle-associated membrane protein, VAMP-2 (also called synaptobrevin-2), linked to either enhanced green fluorescent protein (EGFP) or citrine [a less pH-sensitive variant of enhanced yellow fluorescent protein (EYFP)], in mouse cortical astrocytes. In contrast, the co-expression of VAMP-2-citrine and the lysosomal transporter sialine fused to EGFP resulted in little overlap. Spectral imaging and linear unmixing permit us to fingerprint the expression of spectrally overlapping fluorescent proteins on single secretory organelles in the presence of a spectrally broad autofluorescence. Our technique provides a robust alternative to error-prone dual- or triple colour co-localisation studies.
Published: 2006
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27 results on '"Isabelle Rivals"'

1. Ultrastructural and dynamic studies of the endosomal compartment in Down syndrome

2. Evening sock marks as an adjunct to the clinical prediction of obstructive sleep apnea

3. Reply to ‘Letter to the editor: is maximal diaphragm tissue velocity suited for the assessment of diaphragm contractility?’

4. Clinical features and outcome of patients with primary central nervous system lymphoma admitted to the intensive care unit: a French national expert center experience

5. Ultrafast ultrasound coupled with cervical magnetic stimulation for non‐invasive and non‐volitional assessment of diaphragm contractility

6. One-year survival of patients with high-grade glioma discharged alive from the intensive care unit

7. Is a simplified Finite Element model of the gluteus region able to capture the mechanical response of the internal soft tissues under compression?

8. Decreased respiratory-related postural perturbations at the cervical level under cognitive load

9. Human diaphragm atrophy in amyotrophic lateral sclerosis is not predicted by routine respiratory measures

10. Mandibular advancement reveals long-term suppression of breathing discomfort in patients with obstructive sleep apnea syndrome

11. Etiology and prognosis of acute respiratory failure in patients with primary malignant brain tumors admitted to the intensive care unit

12. New insight in spiral drawing analysis methods - Application to action tremor quantification

13. Measuring Ventilatory Activity with Structured Light Plethysmography (SLP) Reduces Instrumental Observer Effect and Preserves Tidal Breathing Variability in Healthy and COPD

14. Ceramides and sphingomyelinases in senile plaques

15. Structural and Molecular Alterations of Primary Afferent Fibres in the Spinal Dorsal Horn in Vincristine-Induced Neuropathy in Rat

16. A semi-automated computer tool for the analysis of retinal vessel diameter dynamics

17. Desogestrel enhances ventilation in ondine patients: Animal data involving serotoninergic systems

18. Relieving dyspnoea by non-invasive ventilation decreases pain thresholds in amyotrophic lateral sclerosis

19. Modifications of the endosomal compartment in peripheral blood mononuclear cells and fibroblasts from Alzheimer’s disease patients

20. Trisomy for synaptojanin1 in Down syndrome is functionally linked to the enlargement of early endosomes

21. Functional genomics and molecular networks Gene expression regulations in complex diseases: Down syndrome as a case study

22. Purkinje Cell Maturation Participates in the Control of Oligodendrocyte Differentiation: Role of Sonic Hedgehog and Vitronectin

23. Axonal Targeting of the 5-HT1B Serotonin Receptor Relies on Structure-Specific Constitutive Activation

24. Gene expression signature of cerebellar hypoplasia in a mouse model of Down syndrome during postnatal development

25. Classification of human chromosome 21 gene-expression variations in down syndrome: Impact on disease phenotypes

26. Enrichment or depletion of a GO category within a class of genes: which test?

27. Detecting fluorescent protein expression and co-localisation on single secretory vesicles with linear spectral unmixing

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