1. HYPERsol: flash-frozen results from archival FFPE tissue for clinical proteomics
- Author
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John Wojcik, John P. Wilson, Ilyana Ilieva, Benjamin A. Garcia, Darryl J. Pappin, and Dylan M. Marchione
- Subjects
0303 health sciences ,03 medical and health sciences ,0302 clinical medicine ,Formalin fixed paraffin embedded ,Solubilization ,Computer science ,030220 oncology & carcinogenesis ,Protein purification ,Proteome ,Sample processing ,Computational biology ,Proteomics ,030304 developmental biology - Abstract
Massive formalin-fixed, paraffin-embedded (FFPE) tissue archives exist worldwide, representing a potential gold mine for clinical proteomics research. However, current protocols for FFPE proteomics lack standardization, efficiency, reproducibility, and scalability. Here we present High-Yield Protein Extraction and Recovery by direct SOLubilization (HYPERsol), an optimized workflow using adaptive-focused acoustics (AFA) ultrasonication and S-Trap sample processing that enables proteome coverage and quantification from FFPE samples comparable to that achieved from flash-frozen tissue (average R = 0.936).
- Published
- 2019
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