1. Toward intraoperative detection of disseminated tumor cells in lymph nodes with silicon nanowire field effect transistors
- Author
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Andreas Offenhäusser, Duy Tran, Regina Stockmann, Bernhard Wolfrum, Marnie Winter, Benjamin Thierry, Chih-Tsung Yang, Mohammad Pourhassan-Moghaddam, Tran, Duy P, Winter, Marnie A, Wolfrum, Bernhard, Stockmann, Regina, Yang, Chih-Tsung, Pourhassan-Moghaddam, Mohammad, Offenhaüsser, Andreas, and Thierry, Benjamin
- Subjects
medicine.medical_specialty ,Silicon ,Materials science ,Transistors, Electronic ,nano fabrication ,Colorectal cancer ,General Physics and Astronomy ,diagnostic ,Nanotechnology ,02 engineering and technology ,Biosensing Techniques ,Sensitivity and Specificity ,multichannel detection ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Circulating tumor cell ,medicine ,Animals ,General Materials Science ,Lymph node ,Disseminated Tumor Cell ,integrated biosensor ,lymph node metastasis ,Nanowires ,CMOS ,General Engineering ,Cancer ,silicon nanowire on a chip ,Gold standard (test) ,021001 nanoscience & nanotechnology ,medicine.disease ,Neoplastic Cells, Circulating ,medicine.anatomical_structure ,Lymphatic system ,Molecular Diagnostic Techniques ,030220 oncology & carcinogenesis ,intraoperative detection ,Radiology ,Lymph ,Lymph Nodes ,0210 nano-technology - Abstract
Within an hour, as little as one disseminated tumor cell (DTC) per lymph node can be quantitatively detected using an intraoperative biosensing platform based on silicon nanowire field-effect transistors (SiNW FET). It is also demonstrated that the integrated biosensing platform is able to detect the presence of circulating tumor cells (CTCs) in the blood of colorectal cancer patients. The presence of DTCs in lymph nodes and CTCs in peripheral blood is highly significant as it is strongly associated with poor patient prognosis. The SiNW FET sensing platform out-performed in both sensitivity and rapidity not only the current standard method based on pathological examination of tissue sections but also the emerging clinical gold standard based on molecular assays. The possibility to achieve accurate and highly sensitive analysis of the presence of DTCs in the lymphatics within the surgery time frame has the potential to spare cancer patients from an unnecessary secondary surgery, leading to reduced patient morbidity, improving their psychological wellbeing and reducing time spent in hospital. This study demonstrates the potential of nanoscale field-effect technology in clinical cancer diagnostics. Refereed/Peer-reviewed
- Published
- 2016