1. Cytologic detection of Toxoplasma gondii in the cerebrospinal fluid of a dog and in vitro isolation of a unique mouse-virulent recombinant strain
- Author
-
Waléria Borges-Silva, Gideão S Galvão, George Rêgo Albuquerque, M.M. Rezende-Gondim, Daniele de Santana Rocha, and Luis Fernando Pita Gondim
- Subjects
0303 health sciences ,General Veterinary ,biology ,030231 tropical medicine ,Toxoplasma gondii ,Virulence ,medicine.disease ,biology.organism_classification ,Virology ,Toxoplasmosis ,Neospora caninum ,030308 mycology & parasitology ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,Cerebrospinal fluid ,law ,Cytology ,parasitic diseases ,medicine ,Recombinant DNA ,Parasite hosting - Abstract
Parasites resembling Neospora caninum or Toxoplasma gondii were detected by cytologic examination of cerebrospinal fluid (CSF) from a dog with neurologic disease. The dog became severely ill and was euthanized. Canine tissue homogenates were used for direct parasite isolation in cell culture, bioassay in 2 mouse lineages, and PCR. T. gondii was isolated in monkey kidney cells, and species identity was confirmed by PCR. Inoculated parasites were highly virulent for mice, which developed clinical signs and were euthanized immediately. PCR-RFLP for T. gondii using the cultured isolate (TgDgBA22) was conducted with 12 genetic markers, and a unique recombinant strain was identified. Detection of T. gondii by CSF cytology, although described in humans, had not been reported previously in dogs, to our knowledge, and was crucial for the diagnosis of toxoplasmosis in the examined dog.
- Published
- 2021
- Full Text
- View/download PDF