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Start Over Author "Tan Tao University (TTU)" Topic 0303 health sciences
2 results on '"Tan Tao University (TTU)"'

1. Trends in Systems Biology for the Analysis and Engineering of Clostridium acetobutylicum Metabolism

Author

Ngoc-Phuong-Thao Nguyen, Minyeong Yoo, Philippe Soucaille, BBSRC/EPSRC Synthetic Biology Research Centre, School of Life Sciences, Centre for Biomolecular Sciences, University of Nottingham, UK (UON), School of Medicine, Tan Duc e-City, Duc Hoa, Tan Tao University (TTU), Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), Metabolic Explorer Company, European Project: 237942,EC:FP7:PEOPLE,FP7-PEOPLE-ITN-2008,CLOSTNET(2009), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), and Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Microbiology (medical), biologie des systèmes, Clostridium acetobutylicum, Primary metabolism, approche transcriptomique, Commodity chemicals, Systems biology, fluxomique, Proteomics, Microbiology, Metabolic engineering, Industrial Microbiology, 03 medical and health sciences, proteomics, ingénierie métabolique, Virology, Metabolomics, protéomique, Genetic Association Studies, Fluxomics, 030304 developmental biology, 0303 health sciences, biology, 030306 microbiology, fungi, systems biology, Gene Expression Regulation, Bacterial, biology.organism_classification, equipment and supplies, clostridium acetobutylicum, Infectious Diseases, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Metabolic regulation, biocarburant, Biofuels, Mutation, biofuel, Biochemical engineering, Genetic Engineering, metabolic engineering, Metabolic Networks and Pathways
Abstract

Clostridium acetobutylicum has received renewed interest worldwide as a promising producer of biofuels and bulk chemicals such as n-butanol, 1,3-propanediol, 1,3-butanediol, isopropanol, and butyrate. To develop commercial processes for the production of bulk chemicals via a metabolic engineering approach it is necessary to better characterize both the primary metabolism and metabolic regulation of C. acetobutylicum. Here, we review the history of the development of omics studies of C. acetobutylicum, summarize the recent application of quantitative/integrated omics approaches to the physiological analysis and metabolic engineering of this bacterium, and provide directions for future studies to address current challenges.

Published
2020
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2. An efficient method for markerless mutant generation by allelic exchange in Clostridium acetobutylicum and Clostridium saccharobutylicum using suicide vectors

Author

Tom Wilding-Steel, Armin Ehrenreich, Isabelle Meynial-Salles, Minyeong Yoo, Julie Soula, Céline Foulquier, Ching-Ning Huang, Axel Thiel, Wolfgang Liebl, Philippe Soucaille, Ngoc-Phuong-Thao Nguyen, Toulouse Biotechnology Institute (TBI), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM), Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), Tan Tao University (TTU), University of Nottingham, UK (UON), 613802, KBBE Valor Plus, European Project: 237942,EC:FP7:PEOPLE,FP7-PEOPLE-ITN-2008,CLOSTNET(2009), and Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Clostridium acetobutylicum, lcsh:Biotechnology, Mutant, Computational biology, Biotechnologies, Management, Monitoring, Policy and Law, Clostridium saccharobutylicum, upp gene, 5-FU, Restrictionless, Markerless, Gene deletion, Gene replacement, medicine.disease_cause, 01 natural sciences, Applied Microbiology and Biotechnology, Genome, Genetic analysis, lcsh:Fuel, Metabolic engineering, 03 medical and health sciences, lcsh:TP315-360, lcsh:TP248.13-248.65, 010608 biotechnology, medicine, Gene, 0303 health sciences, Mutation, biology, 030306 microbiology, Renewable Energy, Sustainability and the Environment, Chemistry, fungi, equipment and supplies, biology.organism_classification, ddc, General Energy, Functional genomics, Biotechnology
Abstract

Background: Clostridium acetobutylicum and Clostridium saccharobutylicum are Gram-positive, spore-forming, anaerobic bacterium capable of converting various sugars and polysaccharides into solvents (acetone, butanol, and ethanol). The sequencing of their genomes has prompted new approaches to genetic analysis, functional genomics, and metabolic engineering to develop industrial strains for the production of biofuels and bulk chemicals.Results: The method used in this paper to knock-out, knock-in, or edit genes in C. acetobutylicum and C. saccharobutylicum combines an improved electroporation method with the use of (i) restrictionless Δupp (which encodes uracil phosphoribosyl-transferase) strains and (ii) very small suicide vectors containing a markerless deletion/insertion cassette, an antibiotic resistance gene (for the selection of the first crossing-over) and upp (from C. acetobutylicum) for subsequent use as a counterselectable marker with the aid of 5-fluorouracil (5-FU) to promote the second crossing-over. This method was successfully used to both delete genes and edit genes in both C. acetobutylicum and C. saccharobutylicum. Among the edited genes, a mutation in the spo0A gene that abolished solvent formation in C. acetobutylicum was introduced in C. saccharobutylicum and shown to produce the same effect.Conclusions: The method described in this study will be useful for functional genomic studies and for the development of industrial strains for the production of biofuels and bulk chemicals.

Published
2019
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