1. ELISA and UPLC/FLD as Screening and ConfirmatoryTechniques for T-2/Ht-2 Mycotoxin Determination in Cereals
- Author
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Luigi Urbano, Antonio Curiale, Valeria Vita, Giuliana Marchesani, Antonio Eugenio Chiaravalle, Paola D’Agnello, Cinzia Franchino, Marco Iammarino, Francesca Debegnach, and Rita De Pace
- Subjects
Fusarium ,01 natural sciences ,High-performance liquid chromatography ,lcsh:Technology ,lcsh:Chemistry ,03 medical and health sciences ,chemistry.chemical_compound ,UPLC-FLD ,General Materials Science ,T-2/HT-2 toxins ,Mycotoxin ,Instrumentation ,lcsh:QH301-705.5 ,High potential ,030304 developmental biology ,Fluid Flow and Transfer Processes ,cereals ,validation ,0303 health sciences ,Chromatography ,Animal health ,biology ,Chemistry ,lcsh:T ,Process Chemistry and Technology ,010401 analytical chemistry ,General Engineering ,food and beverages ,biology.organism_classification ,lcsh:QC1-999 ,0104 chemical sciences ,Computer Science Applications ,food safety ,lcsh:Biology (General) ,lcsh:QD1-999 ,lcsh:TA1-2040 ,ELISA ,lcsh:Engineering (General). Civil engineering (General) ,lcsh:Physics - Abstract
T-2 and HT-2 toxins are secondary metabolites of various species of Fusarium. These molecules can have high potential toxic effects for human and animal health. In this work, ELISA and ultra performance liquid chromatography with fluorescence detection (UPLC/FLD) were implemented and validated as screening and confirmatory tests for the detection of these two toxins in cereal samples. The developed methods were tested by analyzing 100 samples of cereals by ELISA screening for reducing costs and analysis time and then using UPLC/FLD for confirmation purposes. Both methods met the performance criteria for sensitivity, linearity, selectivity, precision, and ruggedness, as reported in the European Decision No. 2002/657/EC and in Regulation (EC) No. 401/2006. The correlation between ELISA and UPLC/FLD approaches showed good results (r = 0.9056), confirming that these two techniques should be considered to be complementary in the official control activities of cereal and derived products.
- Published
- 2021
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