1. Microbial soluble aromatic prenyltransferases for engineered biosynthesis
- Author
-
Ikuro Abe and He-Ping Chen
- Subjects
0106 biological sciences ,QH301-705.5 ,FPP, farnesyl diphosphate ,Prenyltransferase ,Biomedical Engineering ,Biosynthesis ,01 natural sciences ,Applied Microbiology and Biotechnology ,Enzyme engineering ,Article ,03 medical and health sciences ,chemistry.chemical_compound ,Synthetic biology ,GPP, geranyl diphosphate ,Prenylation ,Structural Biology ,010608 biotechnology ,Genetics ,Biology (General) ,Microbial prenyltransferase ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,DHN, dihydroxynaphthalene ,Natural product ,DMAPP, dimethylallyl diphosphate ,DMSPP, dimethylallyl S-thiolodiphosphate ,Protein engineering ,GSPP, geranyl S- thiolodiphosphate ,DMATS, dimethylallyltryptophan synthase ,Enzyme ,chemistry ,Biochemistry ,RiPP, ribosomally synthesized and posttranslationally modified peptide ,PPP, phytyl pyrophosphate ,IPP, isopentenyl pyrophosphate ,THN, 1,3,6,8-tetrahydroxynaphthalene ,GFPP, geranyl farnesyl diphosphate ,TP248.13-248.65 ,Biotechnology ,PTase, prenyltransferase - Abstract
Prenyltransferase (PTase) enzymes play crucial roles in natural product biosynthesis by transferring isoprene unit(s) to target substrates, thereby generating prenylated compounds. The prenylation step leads to a diverse group of natural products with improved membrane affinity and enhanced bioactivity, as compared to the non-prenylated forms. The last two decades have witnessed increasing studies on the identification, characterization, enzyme engineering, and synthetic biology of microbial PTase family enzymes. We herein summarize several examples of microbial soluble aromatic PTases for chemoenzymatic syntheses of unnatural novel prenylated compounds.
- Published
- 2021