1. Validation of oral brushing as a non‐invasive technique for the identification of feline oral squamous cell carcinoma by DNA methylation and TP53 mutation analysis
- Author
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Marina Aralla, Antonella Rigillo, Luca Morandi, Laura Marconato, Andrea Renzi, Debora Tinto, Giuliano Bettini, Jacopo Lenzi, Silvia Sabattini, Emma Bellei, Renzi A., Morandi L., Bellei E., Marconato L., Rigillo A., Aralla M., Lenzi J., Bettini G., Tinto D., and Sabattini S. more...
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p53 ,Oncology ,medicine.medical_specialty ,040301 veterinary sciences ,Physical examination ,Cat Diseases ,oral brushing ,0403 veterinary science ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,Animals ,Prospective Studies ,Epigenetics ,feline ,Prospective cohort study ,Gene ,DNA methylation ,General Veterinary ,medicine.diagnostic_test ,Squamous Cell Carcinoma of Head and Neck ,business.industry ,Reproducibility of Results ,04 agricultural and veterinary sciences ,Methylation ,oral squamous cell carcinoma ,Feline Oral Squamous Cell Carcinoma ,early diagnosi ,030220 oncology & carcinogenesis ,FLI1 ,Mutation ,Cats ,bisulfite next generation sequencing ,Mouth Neoplasms ,Tumor Suppressor Protein p53 ,business - Abstract
Feline oral squamous cell carcinoma (FOSCC) is a frequent and progressively invasive tumour. Early lesions are difficult to recognize based on the sole clinical examination and may be misinterpreted as non-neoplastic. Mutations of TP53 and epigenetic alterations of specific genes are present in FOSCC and may be early detected. Aim of this prospective study was to investigate the DNA methylation pattern of a 17-gene panel and TP53 mutational status of FOSCC cytological samples obtained by oral brushing. Results were compared with a control group, in order to validate this non-invasive procedure for the screening of FOSCC. In FOSCC, the same analyses were carried out on the corresponding histological sample, if available. Thirty-five FOSCC and 60 controls were included. Mutations of TP53 were detected in 17 FOSCC brushings (48%) and in none of the controls (P more...
- Published
- 2021
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