1. Combined therapeutic efficacy of carvacrol and X-radiation against 1,2-dimethyl hydrazine-induced experimental rat colon carcinogenesis.
- Author
-
Arivalagan S, Thomas NS, Chandrasekaran B, Mani V, Siddique AI, Kuppsamy T, and Namasivayam N
- Subjects
- Animals, Antigens, Nuclear metabolism, Antineoplastic Agents administration & dosage, Colonic Neoplasms chemically induced, Colonic Neoplasms metabolism, Colonic Neoplasms pathology, Cymenes, Drug Administration Schedule, Enzymes metabolism, Male, Mast Cells drug effects, Mast Cells radiation effects, Metabolic Detoxication, Phase I, Metabolic Detoxication, Phase II, Monoterpenes administration & dosage, Mucins metabolism, Neoplasms, Experimental chemically induced, Neoplasms, Experimental metabolism, Neoplasms, Experimental pathology, Radiation Dosage, Rats, Wistar, Time Factors, 1,2-Dimethylhydrazine, Antineoplastic Agents pharmacology, Chemoradiotherapy, Colonic Neoplasms therapy, Monoterpenes pharmacology, Neoplasms, Experimental therapy
- Abstract
Colon cancer is one of the most commonly diagnosed cancers, and is a major cause of cancer morbidity and mortality worldwide. The objective of the present study is to evaluate the combined therapeutic efficacy of carvacrol (CVC) and X-radiation against 1,2-dimethylhydrazine-induced colon cancer. Male albino Wistar rats were randomly divided into six groups. Group 1 served as control; group 2 received 40 mg/kg b.wt of CVC orally everyday throughout the experimental period (32 weeks); groups 3-6 received subcutaneous injections of DMH (20 mg/kg b.wt), once a week for the first 15 weeks; group 4 received a single dose of X-radiation at the 31st week; group 5 received CVC (40 mg/kg b.wt) two days after the last injection of DMH and continued everyday till the end of the experimental period; group 6 received CVC as in group 5 and radiation as in group 4. DMH-treated rats showed increased incidence of aberrant crypt foci (ACF), dysplastic aberrant crypt foci (DACF), mast cell number, argyrophilic nucleolar organizer regions; elevated activities of phase I enzymes, decreased activities of phase II enzymes, decreased mucin content and altered colonic and liver histology as compared to control rats. Though the individual treatments with CVC and X-radiation to DMH-treated rats reversed the above changes, the combined treatment with both CVC and X-radiation showed a marked effect. Our findings emphasize the potential role of combined therapeutic effect of CVC and X-radiation against DMH-induced colon carcinogenesis.
- Published
- 2015
- Full Text
- View/download PDF