1. A protein complex required for polar growth of rhizobial infection threads
- Author
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Liu, Cheng-Wu, Breakspear, Andrew, Stacey, Nicola, Findlay, Kim, Nakashima, Jin, Ramakrishnan, Karunakaran, Liu, Miaoxia, Xie, Fang, Endre, Gabriella, de Carvalho-Niebel, Fernanda, Oldroyd, Giles, Udvardi, Michael, Fournier, Joëlle, Murray, Jeremy, Department of Cell and Developmental Biology, John Innes Centre [Norwich], National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences [Changchun Branch] (CAS), The Samuel Roberts Noble Foundation, Laboratoire des interactions plantes micro-organismes (LIPM), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), National Key R&D Program of China : 2016YFA0500500, Biotechnology and Biological Sciences Research Council : BB/L010305/1, French National Research grants : TULIP ANR-10-LABX-41 (TULIP)/COME-IN ANR-14-CE35-0007-01, Liu, Cheng-Wu [0000-0002-6650-6245], de Carvalho-Niebel, Fernanda [0000-0002-5596-9420], Murray, Jeremy D. [0000-0003-3000-9199], Apollo - University of Cambridge Repository, University of Cambridge [UK] (CAM), Hungarian Academy of Sciences (MTA), Biotechnology and Biological Sciences Research Council (BBSRC) : BB/L010305/1, ANR-10-LABX-0041,TULIP,Towards a Unified theory of biotic Interactions: the roLe of environmental(2010), ANR-14-CE35-0007,COME-IN,Cell-specific reprogramming of legume roots for endosymbiotic infection(2014), Liu, Chengwu [0000-0002-6650-6245], and Oldroyd, Giles [0000-0002-5245-6355]
- Subjects
Science ,Agrobacterium ,631/80/313/1481 ,Plant cell biology ,Plant Root Nodulation ,Plant Roots ,Article ,Exocytosis ,42/44 ,Gene Expression Regulation, Plant ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Two-Hybrid System Techniques ,Medicago truncatula ,631/449/448 ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,631/449/2676/2678 ,14/19 ,Symbiosis ,lcsh:Science ,14/35 ,Plant Proteins ,Rhizobial symbiosis ,Vegetal Biology ,38/111 ,fungi ,food and beverages ,[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,14/63 ,14/28 ,lcsh:Q ,Biologie végétale ,Sinorhizobium meliloti - Abstract
During root nodule symbiosis, intracellular accommodation of rhizobia by legumes is a prerequisite for nitrogen fixation. For many legumes, rhizobial colonization initiates in root hairs through transcellular infection threads. In Medicago truncatula, VAPYRIN (VPY) and a putative E3 ligase LUMPY INFECTIONS (LIN) are required for infection thread development but their cellular and molecular roles are obscure. Here we show that LIN and its homolog LIN-LIKE interact with VPY and VPY-LIKE in a subcellular complex localized to puncta both at the tip of the growing infection thread and at the nuclear periphery in root hairs and that the punctate accumulation of VPY is positively regulated by LIN. We also show that an otherwise nuclear and cytoplasmic exocyst subunit, EXO70H4, systematically co-localizes with VPY and LIN during rhizobial infection. Genetic analysis shows that defective rhizobial infection in exo70h4 is similar to that in vpy and lin. Our results indicate that VPY, LIN and EXO70H4 are part of the symbiosis-specific machinery required for polar growth of infection threads., Many legumes accommodate rhizobial symbionts via transcellular infection threads. Here the authors show that in Medicago root hairs, polar growth of the infection thread requires a tip-localized protein complex consisting of VPY and VPY-like proteins that are stabilized by the E3 ligase LIN, as well as an exocyst complex subunit.
- Published
- 2019