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5 results on '"Hilal Hafian"'

1. Multiphoton Imaging of Tumor Biomarkers in situ Using Single-domain Antibodies Conjugated with Quantum Dots in a Set Orientation

Author

Michel Pluot, Alyona Sukhanova, Jean-Marc Millot, Jacques H. M. Cohen, Igor Nabiev, Daniel Baty, Marc Turini, Hilal Hafian, Patrick Chames, Laboratoire de Recherche en Nanosciences - EA 4682 (LRN), Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche en Cancérologie de Marseille (CRCM), Aix Marseille Université (AMU)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université de Reims Champagne-Ardenne (URCA)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)

Subjects
In situ, two-photon excitation, immunodiagnostic nanoprobes, Photoluminescence, Materials science, [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, single-domain antibodies, Nanotechnology, quantum dots, 02 engineering and technology, Conjugated system, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, 0104 chemical sciences, 3. Good health, Autofluorescence, Quantum dot, Excited state, [SDV.IB]Life Sciences [q-bio]/Bioengineering, Single domain, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, 0210 nano-technology, Multiphoton imaging
Abstract

International audience; The use of photoluminescence (PL) probes in immunodiagnostic procedures requires highly specific and precise labeling of biomarkers and he tags whose PL is easily differentiable from that of the autofluorescence of cells in clinical specimens. Nanoprobes consisting of core/shell quantum dots (QDs), notably CdSe/ZnS ones, and single-domain antibodies (sdAbs) with PL excited in the multiphoton mode meet both requirements. These diagnostic nanoprobes are

Published
2016
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2. Multiphoton imaging of tumor biomarkers in situ using highly oriented conjugates of single-domain antibodies and quantum dots

Author

Alyona Sukhanova, Hilal Hafian, Michel Pluot, Jacques H. M. Cohen, Jean-Marc Millot, Igor Nabiev, Alyona Sukhanava, Marc Turini, Patrick Chames, Daniel Baty, Laboratoire de Recherche en Nanosciences - EA 4682 (LRN), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV), Aix Marseille Université (AMU), Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)

Subjects
In situ, Materials science, medicine.diagnostic_test, [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, Nanotechnology, [SDV.CAN]Life Sciences [q-bio]/Cancer, Conjugated system, Immunofluorescence, Fluorescence, 3. Good health, Nuclear magnetic resonance, Quantum dot, medicine, [SDV.IB]Life Sciences [q-bio]/Bioengineering, Single domain, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, Immunostaining, Conjugate
Abstract

International audience; High-quality imaging for immunofluorescence diagnosis in medical practice requires a high sensitivity of labeling and discrimination between the fluorescence of immunostaining probes and tissue autofluorescence. Multiphoton microscopy with excitation in the near-infrared spectral region, far from the region of excitation of tissue autofluorescence, enables deep imaging of biological tissues. CdSe/ZnS quantum dots (QDs) conjugated to single-domain antibodies (sdAbs) are efficient diagnostic nanoprobes with diameters 40 times higher than that for one-photon excitation.

Published
2015
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3. Immunohistochemical study of DNA topoisomerase I, DNA topoisomerase IIα, p53, and Ki-67 in oral preneoplastic lesions and oral squamous cell carcinomas

Author

Benoît Lefèvre, Igor Nabiev, Hilal Hafian, Lydie Venteo, Michel Pluot, Alyona Sukhanova, Laboratoire Central d'Anatomie et de Cytologie Pathologiques [Hôpital Edouard Herriot - HCL], Hôpital Edouard Herriot [CHU - HCL], Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), Laboratoire de Recherche en Nanosciences - EA 4682 (LRN), Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Adult, Male, medicine.drug_class, Cell, [SDV.CAN]Life Sciences [q-bio]/Cancer, Monoclonal antibody, Pathology and Forensic Medicine, 03 medical and health sciences, 0302 clinical medicine, Antigens, Neoplasm, Biomarkers, Tumor, medicine, Humans, DNA topoisomerase I, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Aged, 030304 developmental biology, 0303 health sciences, biology, Topoisomerase, Middle Aged, oral intraepithelial neoplasia (OIN), DNA topoisomerase II-alpha, Molecular biology, 3. Good health, DNA-Binding Proteins, oral squamous cell carcinoma, DNA Topoisomerases, Type II, Ki-67 Antigen, medicine.anatomical_structure, DNA Topoisomerases, Type I, Epidermoid carcinoma, 030220 oncology & carcinogenesis, Ki-67, immunohistochemistry, Carcinoma, Squamous Cell, biology.protein, Immunohistochemistry, Female, Mouth Neoplasms, Tumor Suppressor Protein p53, Precancerous Conditions, Immunostaining, Camptothecin, medicine.drug
Abstract

International audience; Human DNA topoisomerase I (topo I) is the molecular target ofthe camptothecin group of anticancer drugs. Laboratory studies haveshown that the cellular response to topo I–targeted drugs depends onthe topo I expression and DNA replication rate and the apoptoticpathway activity. In this study, we tested potential indicators of thesensitivity of topo I–targeted drugs in 36 cases of oral squamous cellcarcinoma (OSCC). Formalin-fixed, paraffin-embedded tissue sec-tions were immunostained with monoclonal antibodies against Ki-67,p53, and topo I, and with polyclonal antibodies against DNA topo-isomerase II-alpha (topo II-alpha). These markers were also tested in 18epithelial hyperplastic lesions and 18 mild dysplasias. Immunostain-ing was quantified by the percentage of stained nuclei in each sample (the labeling index); 200 immunoreactive epithelial nuclei werecounted per case for each antibody. The results support the possi-bility of using topo II-alpha staining for assessing the proliferative activ-ity. High expression of topo II-and topo I in OSCCs suggests thatthey may serve as potential indicators of sensitivity to topo I inhibitors. However, the apoptotic pathway assessed by p53 immunostain-ing was found to be uninformative. Analysis of the relationshipbetween immunohistochemical results and clinical and pathologicparameters (the T and N stages and differentiation) showed that onlythe differentiation parameter correlated with the topo I expressionrate. Thus, significant increase in the topo I expression in the poorlydifferentiated OSCCs suggests their higher sensitivity to drugtreatment.

Published
2004
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4. Single-photon and two-photon tumor imaging and diagnosis using oriented conjugates of single-domain antibodies and quantum dots

Author

Z A Sokolova, Hilal Hafian, Patrick Chames, M. A. Baryshnikova, Kristina Brazhnik, Michel Pluot, Regina Bilan, Jean-Marc Millot, Igor Nabiev, Jacques H. M. Cohen, Alyona Sukhanova, Daniel Baty, Laboratoire de Recherche en Nanosciences - EA 4682 (LRN), Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), and The National Research Nuclear University MEPhI (Moscow Engineering Physics Institute) [Moscow, Russia]

Subjects
Physics, Tumor imaging, Photon, 02 engineering and technology, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, 3. Good health, 0104 chemical sciences, Nuclear magnetic resonance, Two-photon excitation microscopy, Quantum dot, [INFO.INFO-IM]Computer Science [cs]/Medical Imaging, Single domain, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, 0210 nano-technology, ComputingMilieux_MISCELLANEOUS, Conjugate
Abstract

International audience

Published
2015

5. Bi-photon imaging and diagnostics using ultra-small diagnostic probes engineered from semiconductor nanocrystals and single-domain antibodies

Author

Michel Pluot, Jacques Cohen, Alyona Sukhanova, Daniel Baty, Jean-Marc Millot, Hilal Hafian, Igor Nabiev, Patrick Chames, Laboratoire de Recherche en Nanosciences - EA 4682 (LRN), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV), Centre de Recherche en Cancérologie de Marseille (CRCM), Aix Marseille Université (AMU)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), and Université de Reims Champagne-Ardenne (URCA)

Subjects
Materials science, Photon, medicine.drug_class, quantum dots, Nanotechnology, 02 engineering and technology, Monoclonal antibody, 01 natural sciences, 010309 optics, 0103 physical sciences, diagnostics, medicine, Semiconductor nanocrystals, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, biology, single-domain antibodies, technology, industry, and agriculture, imaging, equipment and supplies, 021001 nanoscience & nanotechnology, Fluorescence, nanobodies, 3. Good health, Autofluorescence, Quantum dot, immunohistochemistry, nanoprobe, biology.protein, [SDV.IB]Life Sciences [q-bio]/Bioengineering, Antibody, 0210 nano-technology, Conjugate
Abstract

International audience; Semiconductor fluorescent quantum dots (QDs) have just demonstrated their numerous advantages over organic dyes in bioimaging and diagnostics. One of characteristics of QDs is a very large cross section of their two-photon absorption. A common approach to biodetection by means of QDs is to use monoclonal antibodies (mAbs) for targeting. Recently, we have engineered ultrasmall diagnostic nanoprobes (sdAb–QD) based on highly oriented conjugates of QDs with the single-domain antibodies (sdAbs) against cancer biomarkers. With a molecular weight of only 13 kDa (12-fold smaller than full-size mAbs) and extreme stability and capacity to refolding, sdAbs are the smallest functional Ab fragments capable of binding antigens with affinities comparable to those of conventional Abs. Ultrasmall diagnostic sdAb–QD nanoprobes were engineered through oriented conjugation of QDs with sdAbs. This study is the first to demonstrate the possibility of immunohistochemical imaging of colon carcinoma biomarkers with sdAb–QD conjugates by means of two-photon excitation. The optimal excitation conditions for imaging of the markers in clinical samples with sdAb–QD nanoprobes have been determined. The absence of sample autofluorescence significantly improves the sensitivity of biomarker detection with the use of the two-photon excitation diagnostic setup.

Published
2012
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