Search

Your search keyword '"Mamanova, Lira"' showing total 10 results
Start Over Author "Mamanova, Lira" Topic 3. good health
10 results on '"Mamanova, Lira"'

1. Decoding human fetal liver haematopoiesis

Author

Popescu, Dorin-Mirel, Botting, Rachel A, Stephenson, Emily, Green, Kile, Webb, Simone, Jardine, Laura, Calderbank, Emily F, Polanski, Krzysztof, Goh, Issac, Efremova, Mirjana, Acres, Meghan, Maunder, Daniel, Vegh, Peter, Gitton, Yorick, Park, Jong-Eun, Vento-Tormo, Roser, Miao, Zhichao, Dixon, David, Rowell, Rachel, McDonald, David, Fletcher, James, Poyner, Elizabeth, Reynolds, Gary, Mather, Michael, Moldovan, Corina, Mamanova, Lira, Greig, Frankie, Young, Matthew D, Meyer, Kerstin B, Lisgo, Steven, Bacardit, Jaume, Fuller, Andrew, Millar, Ben, Innes, Barbara, Lindsay, Susan, Stubbington, Michael JT, Kowalczyk, Monika S, Li, Bo, Ashenberg, Orr, Tabaka, Marcin, Dionne, Danielle, Tickle, Timothy L, Slyper, Michal, Rozenblatt-Rosen, Orit, Filby, Andrew, Carey, Peter, Villani, Alexandra-Chloé, Roy, Anindita, Regev, Aviv, Chédotal, Alain, Roberts, Irene, Göttgens, Berthold, Behjati, Sam, Laurenti, Elisa, Teichmann, Sarah A, and Haniffa, Muzlifah

Subjects
Blood Cells, Lymphoid Tissue, Gene Expression Profiling, Stem Cells, Flow Cytometry, 3. Good health, Hematopoiesis, Fetus, Cellular Microenvironment, Liver, embryonic structures, Humans, Female, Single-Cell Analysis
Abstract

Definitive haematopoiesis in the fetal liver supports self-renewal and differentiation of haematopoietic stem cells and multipotent progenitors (HSC/MPPs) but remains poorly defined in humans. Here, using single-cell transcriptome profiling of approximately 140,000 liver and 74,000 skin, kidney and yolk sac cells, we identify the repertoire of human blood and immune cells during development. We infer differentiation trajectories from HSC/MPPs and evaluate the influence of the tissue microenvironment on blood and immune cell development. We reveal physiological erythropoiesis in fetal skin and the presence of mast cells, natural killer and innate lymphoid cell precursors in the yolk sac. We demonstrate a shift in the haemopoietic composition of fetal liver during gestation away from being predominantly erythroid, accompanied by a parallel change in differentiation potential of HSC/MPPs, which we functionally validate. Our integrated map of fetal liver haematopoiesis provides a blueprint for the study of paediatric blood and immune disorders, and a reference for harnessing the therapeutic potential of HSC/MPPs., We acknowledge funding from the Wellcome Human Cell Atlas Strategic Science Support (WT211276/Z/18/Z); M.H. is funded by Wellcome (WT107931/Z/15/Z), The Lister Institute for Preventive Medicine and NIHR and Newcastle-Biomedical Research Centre; S.A.T. is funded by Wellcome (WT206194), ERC Consolidator and EU MRG-Grammar awards and; S.B. is funded by Wellcome (WT110104/Z/15/Z) and St. Baldrick’s Foundation; E.L. is funded by a Wellcome Sir Henry Dale and Royal Society Fellowships, European Haematology Association, Wellcome and MRC to the Wellcome-MRC Cambridge Stem Cell Institute and BBSRC.

2. Single-cell transcriptomes from human kidneys reveal the cellular identity of renal tumors

Author

Young, Matthew D, Mitchell, Thomas J, Vieira Braga, Felipe A, Tran, Maxine GB, Stewart, Benjamin J, Ferdinand, John R, Collord, Grace, Botting, Rachel A, Popescu, Dorin-Mirel, Loudon, Kevin W, Vento-Tormo, Roser, Stephenson, Emily, Cagan, Alex, Farndon, Sarah J, Del Castillo Velasco-Herrera, Martin, Guzzo, Charlotte, Richoz, Nathan, Mamanova, Lira, Aho, Tevita, Armitage, James N, Riddick, Antony CP, Mushtaq, Imran, Farrell, Stephen, Rampling, Dyanne, Nicholson, James, Filby, Andrew, Burge, Johanna, Lisgo, Steven, Maxwell, Patrick H, Lindsay, Susan, Warren, Anne Y, Stewart, Grant D, Sebire, Neil, Coleman, Nicholas, Haniffa, Muzlifah, Teichmann, Sarah A, Clatworthy, Menna, and Behjati, Sam

Subjects
Adult, Genetic Variation, Humans, Single-Cell Analysis, Child, Kidney, Transcriptome, Carcinoma, Renal Cell, Wilms Tumor, Kidney Neoplasms, 3. Good health
Abstract

Messenger RNA encodes cellular function and phenotype. In the context of human cancer, it defines the identities of malignant cells and the diversity of tumor tissue. We studied 72,501 single-cell transcriptomes of human renal tumors and normal tissue from fetal, pediatric, and adult kidneys. We matched childhood Wilms tumor with specific fetal cell types, thus providing evidence for the hypothesis that Wilms tumor cells are aberrant fetal cells. In adult renal cell carcinoma, we identified a canonical cancer transcriptome that matched a little-known subtype of proximal convoluted tubular cell. Analyses of the tumor composition defined cancer-associated normal cells and delineated a complex vascular endothelial growth factor (VEGF) signaling circuit. Our findings reveal the precise cellular identities and compositions of human kidney tumors.

3. Single cell derived mRNA signals across human kidney tumors

Author

Young, Matthew D, Mitchell, Thomas J, Custers, Lars, Margaritis, Thanasis, Morales-Rodriguez, Francisco, Kwakwa, Kwasi, Khabirova, Eleonora, Kildisiute, Gerda, Oliver, Thomas R W, De Krijger, Ronald R, Van Den Heuvel-Eibrink, Marry M, Comitani, Federico, Piapi, Alice, Bugallo-Blanco, Eva, Thevanesan, Christine, Burke, Christina, Prigmore, Elena, Ambridge, Kirsty, Roberts, Kenny, Braga, Felipe A Vieira, Coorens, Tim H H, Del Valle, Ignacio, Wilbrey-Clark, Anna, Mamanova, Lira, Stewart, Grant D, Gnanapragasam, Vincent J, Rampling, Dyanne, Sebire, Neil, Coleman, Nicholas, Hook, Liz, Warren, Anne, Haniffa, Muzlifah, Kool, Marcel, Pfister, Stefan M, Achermann, John C, He, Xiaoling, Barker, Roger A, Shlien, Adam, Bayraktar, Omer A, Teichmann, Sarah A, Holstege, Frank C, Meyer, Kerstin B, Drost, Jarno, Straathof, Karin, and Behjati, Sam

Subjects
Adult, Models, Genetic, Gene Expression Regulation, Developmental, Kidney, humanities, Kidney Neoplasms, 3. Good health, Fetus, Humans, RNA, Messenger, RNA-Seq, Single-Cell Analysis, Child, Transcriptome, Algorithms, Signal Transduction
Abstract

Funder: Department of Health, Tumor cells may share some patterns of gene expression with their cell of origin, providing clues into the differentiation state and origin of cancer. Here, we study the differentiation state and cellular origin of 1300 childhood and adult kidney tumors. Using single cell mRNA reference maps of normal tissues, we quantify reference "cellular signals" in each tumor. Quantifying global differentiation, we find that childhood tumors exhibit fetal cellular signals, replacing the presumption of "fetalness" with a quantitative measure of immaturity. By contrast, in adult cancers our assessment refutes the suggestion of dedifferentiation towards a fetal state in most cases. We find an intimate connection between developmental mesenchymal populations and childhood renal tumors. We demonstrate the diagnostic potential of our approach with a case study of a cryptic renal tumor. Our findings provide a cellular definition of human renal tumors through an approach that is broadly applicable to human cancer.

4. Mapping Rora expression in resting and activated CD4+ T cells

Author

Haim-Vilmovsky, Liora, Henriksson, Johan, Walker, Jennifer A., Miao, Zhichao, Natan, Eviatar, Kar, Gozde, Clare, Simon, Barlow, Jillian L., Charidemou, Evelina, Mamanova, Lira, Chen, Xi, Proserpio, Valentina, Pramanik, Jhuma, Woodhouse, Steven, Protasio, Anna V., Efremova, Mirjana, Griffin, Julian L., Berriman, Matt, Dougan, Gordon, Fisher, Jasmin, Marioni, John C., McKenzie, Andrew N. J., and Teichmann, Sarah A.

Subjects
Medicine and health sciences, Biology and life sciences, 3. Good health, Research Article
Abstract

Funder: European Bioinformatics Institute; funder-id: http://dx.doi.org/10.13039/100012116, Funder: Fondazione Umberto Veronesi; funder-id: http://dx.doi.org/10.13039/501100004710, The transcription factor Rora has been shown to be important for the development of ILC2 and the regulation of ILC3, macrophages and Treg cells. Here we investigate the role of Rora across CD4+ T cells in general, but with an emphasis on Th2 cells, both in vitro as well as in the context of several in vivo type 2 infection models. We dissect the function of Rora using overexpression and a CD4-conditional Rora-knockout mouse, as well as a RORA-reporter mouse. We establish the importance of Rora in CD4+ T cells for controlling lung inflammation induced by Nippostrongylus brasiliensis infection, and have measured the effect on downstream genes using RNA-seq. Using a systematic stimulation screen of CD4+ T cells, coupled with RNA-seq, we identify upstream regulators of Rora, most importantly IL-33 and CCL7. Our data suggest that Rora is a negative regulator of the immune system, possibly through several downstream pathways, and is under control of the local microenvironment.

5. Somatic mutations and single-cell transcriptomes reveal the root of malignant rhabdoid tumours

Author

Custers, Lars, Khabirova, Eleonora, Coorens, Tim H. H., Oliver, Thomas R. W., Calandrini, Camilla, Young, Matthew D., Vieira Braga, Felipe A., Ellis, Peter, Mamanova, Lira, Segers, Heidi, Maat, Arie, Kool, Marcel, Hoving, Eelco W., Van Den Heuvel-Eibrink, Marry M., Nicholson, James, Straathof, Karin, Hook, Liz, De Krijger, Ronald R., Trayers, Claire, Allinson, Kieren, Behjati, Sam, and Drost, Jarno

Subjects
631/1647/767/70, 45, 631/1647/2217, 38/23, article, 631/337/2019, 13/106, 38/39, 631/67/1678, 13, 3. Good health, 38/91
Abstract

Malignant rhabdoid tumour (MRT) is an often lethal childhood cancer that, like many paediatric tumours, is thought to arise from aberrant fetal development. The embryonic root and differentiation pathways underpinning MRT are not firmly established. Here, we study the origin of MRT by combining phylogenetic analyses and single-cell mRNA studies in patient-derived organoids. Comparison of somatic mutations shared between cancer and surrounding normal tissues places MRT in a lineage with neural crest-derived Schwann cells. Single-cell mRNA readouts of MRT differentiation, which we examine by reverting the genetic driver mutation underpinning MRT, SMARCB1 loss, suggest that cells are blocked en route to differentiating into mesenchyme. Quantitative transcriptional predictions indicate that combined HDAC and mTOR inhibition mimic MRT differentiation, which we confirm experimentally. Our study defines the developmental block of MRT and reveals potential differentiation therapies.

6. Mapping Rora expression in resting and activated CD4+ T cells

Author

Haim-Vilmovsky, Liora, Henriksson, Johan, Walker, Jennifer A, Miao, Zhichao, Natan, Eviatar, Kar, Gozde, Clare, Simon, Barlow, Jillian L, Charidemou, Evelina, Mamanova, Lira, Chen, Xi, Proserpio, Valentina, Pramanik, Jhuma, Woodhouse, Steven, Protasio, Anna V, Efremova, Mirjana, Griffin, Julian L, Berriman, Matt, Dougan, Gordon, Fisher, Jasmin, Marioni, John C, McKenzie, Andrew N J, and Teichmann, Sarah A

Subjects
3. Good health
Abstract

The transcription factor Rora has been shown to be important for the development of ILC2 and the regulation of ILC3, macrophages and Treg cells. Here we investigate the role of Rora across CD4+ T cells in general, but with an emphasis on Th2 cells, both in vitro as well as in the context of several in vivo type 2 infection models. We dissect the function of Rora using overexpression and a CD4-conditional Rora-knockout mouse, as well as a RORA-reporter mouse. We establish the importance of Rora in CD4+ T cells for controlling lung inflammation induced by Nippostrongylus brasiliensis infection, and have measured the effect on downstream genes using RNA-seq. Using a systematic stimulation screen of CD4+ T cells, coupled with RNA-seq, we identify upstream regulators of Rora, most importantly IL-33 and CCL7. Our data suggest that Rora is a negative regulator of the immune system, possibly through several downstream pathways, and is under control of the local microenvironment.

7. Somatic mutations and single-cell transcriptomes reveal the root of malignant rhabdoid tumours

Author

Heidi Segers, James Nicholson, Marry M. van den Heuvel-Eibrink, Liz Hook, Thomas R. W. Oliver, Arie Maat, Matthew D. Young, Lira Mamanova, Tim H. H. Coorens, Karin Straathof, Claire Trayers, Felipe A. Vieira Braga, Eleonora Khabirova, Lars Custers, Marcel Kool, Sam Behjati, Ronald R. de Krijger, Eelco W. Hoving, Camilla Calandrini, Kieren Allinson, Jarno Drost, Peter R. Ellis, Custers, Lars [0000-0003-0252-7714], Khabirova, Eleonora [0000-0002-5891-6789], Coorens, Tim H. H. [0000-0002-5826-3554], Oliver, Thomas R. W. [0000-0003-4306-0102], Vieira Braga, Felipe A. [0000-0003-0206-9258], Mamanova, Lira [0000-0003-1463-8622], Segers, Heidi [0000-0002-3604-7850], Hoving, Eelco W. [0000-0002-5587-0439], van den Heuvel-Eibrink, Marry M. [0000-0002-7760-879X], Straathof, Karin [0000-0001-9673-8568], Trayers, Claire [0000-0003-0236-6041], Behjati, Sam [0000-0002-6600-7665], Drost, Jarno [0000-0002-2941-6179], Apollo - University of Cambridge Repository, Coorens, Tim HH [0000-0002-5826-3554], Oliver, Thomas RW [0000-0003-4306-0102], Vieira Braga, Felipe A [0000-0003-0206-9258], Hoving, Eelco W [0000-0002-5587-0439], van den Heuvel-Eibrink, Marry M [0000-0002-7760-879X], Center of Experimental and Molecular Medicine, and AGEM - Amsterdam Gastroenterology Endocrinology Metabolism

Subjects
0301 basic medicine, Somatic cell, General Physics and Astronomy, 13, medicine.disease_cause, Genomic analysis, Tissue Culture Techniques, 631/1647/767/70, 0302 clinical medicine, 631/1647/2217, 38/23, Phylogeny, Mutation, Multidisciplinary, TOR Serine-Threonine Kinases, article, Cell Differentiation, SMARCB1 Protein, 3. Good health, Gene Expression Regulation, Neoplastic, Malignant rhabdoid tumour, medicine.anatomical_structure, Neural Crest, 030220 oncology & carcinogenesis, embryonic structures, DNA methylation, 38/39, Single-Cell Analysis, animal structures, Science, Mesenchyme, 631/337/2019, 13/106, 631/67/1678, Biology, General Biochemistry, Genetics and Molecular Biology, 38/91, 03 medical and health sciences, Embryonal neoplasms, medicine, Humans, Cancer models, Transcriptomics, Rhabdoid Tumor, PI3K/AKT/mTOR pathway, 45, Gene Expression Profiling, General Chemistry, DNA Methylation, medicine.disease, Embryonic stem cell, Histone Deacetylase Inhibitors, Gene expression profiling, 030104 developmental biology, Cancer research, Drug Screening Assays, Antitumor
Abstract

Malignant rhabdoid tumour (MRT) is an often lethal childhood cancer that, like many paediatric tumours, is thought to arise from aberrant fetal development. The embryonic root and differentiation pathways underpinning MRT are not firmly established. Here, we study the origin of MRT by combining phylogenetic analyses and single-cell mRNA studies in patient-derived organoids. Comparison of somatic mutations shared between cancer and surrounding normal tissues places MRT in a lineage with neural crest-derived Schwann cells. Single-cell mRNA readouts of MRT differentiation, which we examine by reverting the genetic driver mutation underpinning MRT, SMARCB1 loss, suggest that cells are blocked en route to differentiating into mesenchyme. Quantitative transcriptional predictions indicate that combined HDAC and mTOR inhibition mimic MRT differentiation, which we confirm experimentally. Our study defines the developmental block of MRT and reveals potential differentiation therapies., Malignant rhabdoid tumours (MRT) have been suggested to originate in the ectoderm-derived neural crest. Here, the authors analyse MRTs using phylogenetics, scRNA-seq, and patient-derived organoids; they find evidence for an MRT origin in the neural crest lineage and suggest differentiation treatment with HDAC/mTOR inhibitors.

Published
2021
Full Text
View/download PDF

8. Single cell derived mRNA signals across human kidney tumors

Author

Eleonora Khabirova, Vincent J. Gnanapragasam, Tim H. H. Coorens, Adam Shlien, Nicholas Coleman, Omer Ali Bayraktar, Gerda Kildisiute, Muzlifah Haniffa, Federico Comitani, Roger A. Barker, Neil J. Sebire, Christine Thevanesan, Christina Burke, Kerstin B. Meyer, Stefan M. Pfister, Lars Custers, Dyanne Rampling, Karin Straathof, Elena Prigmore, Sarah A. Teichmann, Eva Bugallo-Blanco, Sam Behjati, Anne Y. Warren, Marry M. van den Heuvel-Eibrink, Thomas Rw Oliver, Ronald R. de Krijger, Ignacio del Valle, John Achermann, Anna Wilbrey-Clark, Kenny Roberts, Xiaoling He, Marcel Kool, Alice Piapi, Thanasis Margaritis, Jarno Drost, Felipe A. Vieira Braga, Kwasi Kwakwa, Kirsty Ambridge, Frank C. P. Holstege, Francisco Morales, Matthew D. Young, Thomas J. Mitchell, Grant D. Stewart, Liz Hook, Lira Mamanova, Young, Matthew D [0000-0003-0937-5290], Mitchell, Thomas J [0000-0003-0761-9503], Custers, Lars [0000-0003-0252-7714], Khabirova, Eleonora [0000-0002-5891-6789], Kildisiute, Gerda [0000-0002-5314-2294], Oliver, Thomas RW [0000-0003-4306-0102], van den Heuvel-Eibrink, Marry M [0000-0002-7760-879X], Comitani, Federico [0000-0003-3226-1179], Piapi, Alice [0000-0001-8251-3309], Burke, Christina [0000-0001-5381-3185], Roberts, Kenny [0000-0001-6155-0821], Coorens, Tim HH [0000-0002-5826-3554], Mamanova, Lira [0000-0003-1463-8622], Stewart, Grant D [0000-0003-3188-9140], Warren, Anne [0000-0002-1170-7867], Haniffa, Muzlifah [0000-0002-3927-2084], Achermann, John C [0000-0001-8787-6272], Bayraktar, Omer A [0000-0001-6055-277X], Teichmann, Sarah A [0000-0002-6294-6366], Holstege, Frank C [0000-0002-8090-5146], Meyer, Kerstin B [0000-0001-5906-1498], Drost, Jarno [0000-0002-2941-6179], Straathof, Karin [0000-0001-9673-8568], Behjati, Sam [0000-0002-6600-7665], Apollo - University of Cambridge Repository, Oliver, Thomas R W [0000-0003-4306-0102], Coorens, Tim H H [0000-0002-5826-3554], and Sebire, Neil [0000-0001-5348-9063]

Subjects
0301 basic medicine, Cell of origin, Cell, General Physics and Astronomy, Kidney, Transcriptome, 0302 clinical medicine, Gene expression, Cancer genomics, RNA-Seq, Child, Regulation of gene expression, 0303 health sciences, Multidisciplinary, Gene Expression Regulation, Developmental, Kidney Neoplasms, 3. Good health, medicine.anatomical_structure, 030220 oncology & carcinogenesis, Single-Cell Analysis, Algorithms, Signal Transduction, Adult, Science, Biology, General Biochemistry, Genetics and Molecular Biology, Article, 03 medical and health sciences, Fetus, medicine, Humans, RNA, Messenger, Transcriptomics, 030304 developmental biology, Messenger RNA, Models, Genetic, Mesenchymal stem cell, RNA, Kidney metabolism, Cancer, General Chemistry, medicine.disease, Clear cell renal cell carcinoma, 030104 developmental biology, Cancer research, 030217 neurology & neurosurgery
Abstract

Tumor cells may share some patterns of gene expression with their cell of origin, providing clues into the differentiation state and origin of cancer. Here, we study the differentiation state and cellular origin of 1300 childhood and adult kidney tumors. Using single cell mRNA reference maps of normal tissues, we quantify reference “cellular signals” in each tumor. Quantifying global differentiation, we find that childhood tumors exhibit fetal cellular signals, replacing the presumption of “fetalness” with a quantitative measure of immaturity. By contrast, in adult cancers our assessment refutes the suggestion of dedifferentiation towards a fetal state in most cases. We find an intimate connection between developmental mesenchymal populations and childhood renal tumors. We demonstrate the diagnostic potential of our approach with a case study of a cryptic renal tumor. Our findings provide a cellular definition of human renal tumors through an approach that is broadly applicable to human cancer., Transcriptomic analysis may provide information about the differentiation state and cell of origin of a cancer. Here, the authors assess mRNA signals in 1300 childhood and adult renal tumors and report a fetal origin of childhood tumors and no dedifferentiation of adult tumors.

Published
2020

9. Single-cell transcriptomes from human kidneys reveal the cellular identity of renal tumors

Author

Patrick H. Maxwell, Imran Mushtaq, Antony C. P. Riddick, Neil J. Sebire, Dyanne Rampling, Maxine G. B. Tran, Martin Del Castillo Velasco-Herrera, Steven Lisgo, John R. Ferdinand, Rachel A. Botting, Grant D. Stewart, Roser Vento-Tormo, Nicholas Coleman, Matthew D. Young, James Nicholson, Sarah J. Farndon, Menna R. Clatworthy, Charlotte Guzzo, James N. Armitage, Andrew Filby, Sam Behjati, Dorin-Mirel Popescu, Susan Lindsay, Sarah A. Teichmann, Lira Mamanova, Emily Stephenson, Anne Y. Warren, Alex Cagan, Nathan Richoz, Grace Collord, Muzlifah Haniffa, Thomas J. Mitchell, Stephen Farrell, Benjamin J. Stewart, Johanna Burge, Kevin W. Loudon, Felipe A. Vieira Braga, Tevita Aho, Young, Matthew D [0000-0003-0937-5290], Mitchell, Thomas J [0000-0003-0761-9503], Vieira Braga, Felipe A [0000-0003-0206-9258], Tran, Maxine GB [0000-0002-6034-4433], Stewart, Benjamin J [0000-0003-4522-0085], Ferdinand, John R [0000-0003-0936-0128], Collord, Grace [0000-0003-1924-4411], Stephenson, Emily [0000-0002-4244-4019], Farndon, Sarah J [0000-0001-6024-4267], Del Castillo Velasco-Herrera, Martin [0000-0001-5956-0211], Guzzo, Charlotte [0000-0003-3742-5961], Mamanova, Lira [0000-0003-1463-8622], Lisgo, Steven [0000-0001-5186-3971], Lindsay, Susan [0000-0003-2980-4582], Warren, Anne Y [0000-0002-1170-7867], Stewart, Grant D [0000-0003-3188-9140], Haniffa, Muzlifah [0000-0002-3927-2084], Teichmann, Sarah A [0000-0002-6294-6366], Clatworthy, Menna [0000-0002-3340-9828], Behjati, Sam [0000-0002-6600-7665], Apollo - University of Cambridge Repository, and Graduate School

Subjects
0301 basic medicine, Adult, Cell, Biology, Kidney, Wilms Tumor, Transcriptome, 03 medical and health sciences, chemistry.chemical_compound, Single-cell analysis, Renal cell carcinoma, medicine, Humans, Child, Carcinoma, Renal Cell, Multidisciplinary, Kidney metabolism, Cancer, Genetic Variation, Wilms' tumor, medicine.disease, Kidney Neoplasms, 3. Good health, Vascular endothelial growth factor, 030104 developmental biology, medicine.anatomical_structure, chemistry, Cancer research, Single-Cell Analysis
Abstract

Pediatric and adult kidney tumors differUnderstanding tumor origins and the similarities and differences between organ-specific cancers is important for determining treatment options. Younget al.generated more than 72,000 single-cell transcriptomes from healthy and cancerous human kidneys. From these data, they determined that Wilms tumor, a pediatric kidney cancer, originates from aberrant fetal cells, whereas adult kidney cancers are likely derived from a specific subtype of proximal convoluted tubular cell.Science, this issue p.594

Published
2018

10. Spatiotemporal immune zonation of the human kidney

Author

Dorin-Mirel Popescu, Steven Lisgo, Nathan Richoz, Grace Collord, Anne Y. Warren, James Nicholson, Sarah J. Farndon, Andrew Filby, Roser Vento-Tormo, Marc Bajénoff, Tevita Aho, Gordon L. Frazer, Kevin W. Loudon, Rachel A. Botting, Matthew D. Young, Joy Ursula Lauren Staniforth, Grant D. Stewart, Martin Del Castillo Velasco-Herrera, Benjamin J. Stewart, Stephen Farrell, Nicholas Coleman, Charlotte Guzzo, James N. Armitage, Menna R. Clatworthy, Emily Stephenson, Johanna Burge, Felipe A. Vieira Braga, Muzlifah Haniffa, Imran Mushtaq, Neil J. Sebire, Thomas J. Mitchell, Kile Green, Susan Lindsay, Lira Mamanova, Krzysztof Polanski, Sam Behjati, Alex Cagan, Alexandra M. Riding, Antony C. P. Riddick, John R. Ferdinand, Dyanne Rampling, Sarah A. Teichmann, Mirjana Efremova, Defence Science and Technology Organisation (DSTO), Australian Government, Great Ormond Street Hospital for Children [London] (GOSH), Newcastle University [Newcastle], Institute of Genetic Medicine, Centre d'Immunologie de Marseille - Luminy (CIML), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Department of Histopathology & Pediatric Laboratory Medicine, Great Ormond Street Hospital, Institute of Cellular Medicine [Newcastle], Stewart, Benjamin J [0000-0003-4522-0085], Ferdinand, John R [0000-0003-0936-0128], Young, Matthew D [0000-0003-0937-5290], Mitchell, Thomas J [0000-0003-0761-9503], Loudon, Kevin W [0000-0001-9055-6894], Riding, Alexandra M [0000-0002-6915-5671], Staniforth, Joy UL [0000-0003-2817-0098], Vieira Braga, Felipe A [0000-0003-0206-9258], Botting, Rachel A [0000-0001-9595-4605], Stephenson, Emily [0000-0002-4244-4019], Cagan, Alex [0000-0002-7857-4771], Farndon, Sarah J [0000-0001-6024-4267], Polanski, Krzysztof [0000-0002-2586-9576], Del Castillo Velasco-Herrera, Martin [0000-0001-5956-0211], Guzzo, Charlotte [0000-0003-3742-5961], Collord, Grace [0000-0003-1924-4411], Mamanova, Lira [0000-0003-1463-8622], Aho, Tevita [0000-0001-8456-9285], Mushtaq, Imran [0000-0002-7930-0342], Lisgo, Steven [0000-0001-5186-3971], Lindsay, Susan [0000-0003-2980-4582], Stewart, Grant D [0000-0003-3188-9140], Haniffa, Muzlifah [0000-0002-3927-2084], Teichmann, Sarah A [0000-0002-6294-6366], Behjati, Sam [0000-0002-6600-7665], Clatworthy, Menna R [0000-0002-3340-9828], Apollo - University of Cambridge Repository, and Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Adult, Myeloid, Neutrophils, Transgene, Mice, Transgenic, Biology, Kidney, [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity, Article, 03 medical and health sciences, 0302 clinical medicine, Immune system, Fetus, Single-cell analysis, medicine, Animals, Humans, Myeloid Cells, Lymphocytes, RNA-Seq, 030304 developmental biology, Regulation of gene expression, 0303 health sciences, Multidisciplinary, Macrophages, Gene Expression Regulation, Developmental, Epithelial Cells, biochemical phenomena, metabolism, and nutrition, Epithelium, 3. Good health, Cell biology, Mice, Inbred C57BL, medicine.anatomical_structure, Urinary Tract Infections, bacteria, Female, Single-Cell Analysis, 030217 neurology & neurosurgery, Homeostasis
Abstract

Immune landscape of the human kidney Single-cell RNA sequencing has begun to shed light on the full cellular diversity of specific organs. However, these studies rarely examine organ-specific immune cells. Stewart et al. sequenced healthy adult and fetal kidney samples at a single-cell level to define the heterogeneity in epithelial, myeloid, and lymphoid cells. From this dataset, they identified zonation of cells, with relevance to disease and the varied perturbations that occur in different tumor settings. This profiling of the human kidney generates a comprehensive census of existing cell populations that will help inform the diagnosis and treatment of kidney-related diseases. Science , this issue p. 1461

Published
2018
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results