1. Author Correction: Surfaceome dynamics reveal proteostasis-independent reorganization of neuronal surface proteins during development and synaptic plasticity
- Author
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Benjamin Campbell, Patrick G. A. Pedrioli, Shiva K. Tyagarajan, Marc van Oostrum, Charlotte Seng, Bernd Wollscheid, Csaba Földy, Maik Müller, Jacqueline Hammer, Susanne tom Dieck, University of Zurich, and Wollscheid, Bernd
- Subjects
Science ,Long-Term Potentiation ,General Physics and Astronomy ,10050 Institute of Pharmacology and Toxicology ,610 Medicine & health ,1600 General Chemistry ,Synaptic plasticity ,General Biochemistry, Genetics and Molecular Biology ,Protein protein interaction network ,1300 General Biochemistry, Genetics and Molecular Biology ,Animals ,Homeostasis ,Protein Interaction Maps ,Author Correction ,lcsh:Science ,Cells, Cultured ,Neurons ,Neuronal Plasticity ,Multidisciplinary ,Mass spectrometry ,Chemistry ,Cell Membrane ,Dynamics (mechanics) ,Excitatory Postsynaptic Potentials ,Membrane Proteins ,General Chemistry ,3100 General Physics and Astronomy ,Protein-protein interaction networks ,Rats ,Protein Transport ,Proteostasis ,Synapses ,Neuronal development ,570 Life sciences ,biology ,lcsh:Q ,Neuroscience - Abstract
Neurons are highly compartmentalized cells with tightly controlled subcellular protein organization. While brain transcriptome, connectome and global proteome maps are being generated, system-wide analysis of temporal protein dynamics at the subcellular level are currently lacking. Here, we perform a temporally-resolved surfaceome analysis of primary neuron cultures and reveal dynamic surface protein clusters that reflect the functional requirements during distinct stages of neuronal development. Direct comparison of surface and total protein pools during development and homeostatic synaptic scaling demonstrates system-wide proteostasis-independent remodeling of the neuronal surface, illustrating widespread regulation on the level of surface trafficking. Finally, quantitative analysis of the neuronal surface during chemical long-term potentiation (cLTP) reveals fast externalization of diverse classes of surface proteins beyond the AMPA receptor, providing avenues to investigate the requirement of exocytosis for LTP. Our resource (neurosurfaceome.ethz.ch) highlights the importance of subcellular resolution for systems-level understanding of cellular processes.
- Published
- 2020