1. Wheat CBL-interacting protein kinase 23 positively regulates drought stress and ABA responses.
- Author
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Cui, Xiao-Yu, Du, Yong-Tao, Fu, Jin-dong, Yu, Tai-Fei, Wang, Chang-Tao, Chen, Ming, Chen, Jun, Ma, You-Zhi, and Xu, Zhao-Shi
- Subjects
CALCINEURIN ,PROTEIN kinases ,CROPS ,DROUGHT tolerance ,ABIOTIC stress ,WHEAT ,CELL communication ,PLANT physiology ,PHYSIOLOGY - Abstract
Background: The calcineurin B-like protein (CBL)-interacting protein kinase (CIPK) signaling pathway responds to various abiotic stresses in plants. Results: Wheat
CIPK23, isolated from wheat drought transcriptome data set, was induced by multiple abiotic stresses, including drought, salt, and abscisic acid (ABA). Compared with wild-type plants,TaCIPK23 -overexpression wheat andArabidopsis showed an higher survival rate under drought conditions with enhanced germination rate, developed root system, increased accumulation of osmolytes, and reduced water loss rate. Over-expression ofTaCIPK23 rendered transgenic plants ABA sensitivity, as evidenced by delayed seed germination and the induction of stomatal closure. Consistent with the ABA-sensitive phenotype, the expression level of drought- and ABA-responsive genes were increased under drought conditions in the transgenic plants. In addition, using yeast two-hybrid system, pull-down and bimolecular fluorescence complementation (BiFc) assays, TaCIPK23 was found to interact with TaCBL1 on the plasma membrane. Conclusions: These results suggest thatTaCIPK23 plays important roles in ABA and drought stress responses, and mediates crosstalk between the ABA signaling pathway and drought stress responses in wheat. [ABSTRACT FROM AUTHOR]- Published
- 2018
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