1. Ethylene response factor Sl-ERFB3 is responsive to abiotic stresses and mediates salt and cold stress response regulation in tomato
- Author
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Mondher Bouzayen, Imen Klay, Anne Bernadac, Ameur Cherif, Julien Pirrello, Sadok Bouzid, Leila Riahi, Université Fédérale Toulouse Midi-Pyrénées, Université of Tunis, Institut National de la Recherche Agronomique (INRA), Génomique et Biotechnologie des Fruits (GBF), Institut National de la Recherche Agronomique (INRA)-École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Université de la Manouba [Tunisie] (UMA), Institut National Polytechnique de Toulouse - INPT (FRANCE), Institut National de la Recherche Agronomique - INRA (FRANCE), Université de la Manouba - UMA (TUNISIA), Université de Tunis - El Manar (TUNISIA), Génomique et Biotechnologie des Fruits - GBF (Castanet-Tolosan, France), Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE), and Klay, Imen
- Subjects
hormone régulatrice ,Salinity ,lcsh:Medicine ,lcsh:Technology ,tomate ,Solanum lycopersicum ,Gene Expression Regulation, Plant ,lcsh:Science ,Plant Proteins ,General Environmental Science ,Abiotic component ,Regulation of gene expression ,biology ,Temperature ,food and beverages ,Salt Tolerance ,General Medicine ,[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics ,Plants, Genetically Modified ,Plant hormones ethylene ,Droughts ,Cell biology ,Botanique ,Phenotype ,Shoot ,Research Article ,Botanics ,Article Subject ,stress salin ,Molecular Sequence Data ,RT-PCR ,nicotiana tabacum ,Biotechnologies ,General Biochemistry, Genetics and Molecular Biology ,physiologie végétale ,Stress, Physiological ,Botany ,Genetically modified tomato ,Amino Acid Sequence ,salinité ,stress abiotique ,lcsh:T ,Abiotic stress ,stress au froid ,lcsh:R ,fungi ,Wild type ,biology.organism_classification ,facteur de régulation ,éthylène ,Oxidative stress ,gène antisens ,plante transgénique ,lcsh:Q ,Ethylene signal ,Solanum ,Sequence Alignment ,hormone végétale ,Transcription Factors - Abstract
Sl-ERF.B.3 (Solanum lycopersicum ethylene response factor B.3)gene encodes for a tomato transcription factor of the ERF (ethylene responsive factor) family. Our results of real-time RT-PCR showed thatSl-ERF.B.3is an abiotic stress responsive gene, which is induced by cold, heat, and flooding, but downregulated by salinity and drought. To get more insight into the role ofSl-ERF.B.3in plant response to separate salinity and cold, a comparative study between wild type and twoSl-ERF.B.3antisense transgenic tomato lines was achieved. Compared with wild type,Sl-ERF.B.3antisense transgenic plants exhibited a salt stress dependent growth inhibition. This inhibition was significantly enhanced in shoots but reduced in roots, leading to an increased root to shoot ratio. Furthermore, the cold stress essay clearly revealed that introducing antisenseSl-ERF.B.3in transgenic tomato plants reduces their cell injury and enhances their tolerance against 14 d of cold stress. All these results suggest thatSl-ERF.B.3gene is involved in plant response to abiotic stresses and may play a role in the layout of stress symptoms under cold stress and in growth regulation under salinity.
- Published
- 2014
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