1. ABO Antigens Active Tri- and Disaccharides Microarray to Evaluate C-type Lectin Receptor Binding Preferences.
- Author
-
Shanthamurthy CD, Jain P, Yehuda S, Monteiro JT, Leviatan Ben-Arye S, Subramani B, Lepenies B, Padler-Karavani V, and Kikkeri R
- Subjects
- Animals, Disaccharides chemistry, Disaccharides immunology, Glycosylation, Humans, Mice, Molecular Structure, Polysaccharides chemistry, Protein Array Analysis, Protein Binding immunology, ABO Blood-Group System immunology, ABO Blood-Group System metabolism, Lectins, C-Type metabolism, Polysaccharides immunology
- Abstract
Understanding blood group antigen binding preferences for C-type lectin receptors holds promise for modulating immune responses, since several Gram-negative bacteria express blood group antigens as molecular mimicry to evade immune responses. Herein, we report the synthesis of ABO blood group antigen active tri and disaccharides to investigate the binding specificity with various C-type lectin receptors using glycan microarray. The results of binding preferences show that distinct glycosylation on the galactose and fucose motifs are key for C-type lectin receptor binding and that these interactions occur in a Ca
2+ -dependent fashion.- Published
- 2018
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