1. Upregulation of Pro-inflammatory Cytokine Expression Following Chronic Paracetamol Treatment in Astrocyte.
- Author
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Tantarungsee N, Yisarakun W, Thongtan T, Lalert L, Srikam S, Reuangwechvorachai P, Ingruanglert P, and Maneesri-le Grand S
- Subjects
- Animals, Astrocytes ultrastructure, Cell Line, Transformed, Dose-Response Relationship, Drug, Mice, NF-kappa B metabolism, Time Factors, Acetaminophen pharmacology, Analgesics, Non-Narcotic pharmacology, Astrocytes drug effects, Interleukin-1beta metabolism, Tumor Necrosis Factor-alpha metabolism, Up-Regulation drug effects
- Abstract
The present study aimed to investigate the effect of APAP treatment on the expression of pro-inflammatory cytokines in the astrocytes. The mouse astrocyte cells (C8-D1A) were treated with APAP at the concentration of 100 μM for 24 h, 16 and 28 days. The expressions of pro-inflammatory cytokines and NF-kB were determined using western blot analysis. Furthermore, the expression and localization of phosphorylation of NF-kB were detected by immunohistochemical and immunofluorescent analysis. The ultrastructure of C8-D1A cells was as well monitored. The results revealed that acute APAP treatment (24 h) had no effect on the expression of pro-inflammatory cytokines and pNF-kB. This treatment did not alter the ultrastructure of C8-D1A cells when compared with those in the control cells. However, the results obtained from the study on chronic APAP-treated cells (16 and 28 days) showed the different effect of APAP treatment. The results obtained from western blot analysis showed the increment of pro-inflammatory cytokine (IL-1β and TNF-α) expressions and the activation of NF-kB signaling pathway. Nuclear translocation of pNF-kB and alteration of several cell structures were well observed in the C8-D1A cells with chronic APAP treatment. The results obtained from this study suggest that chronic APAP treatment can induce an upregulation of pro-inflammatory cytokines (IL-1β and TNFα) in astrocytes. This alteration implies the involvement of the activation of NF-kB signaling pathway.
- Published
- 2018
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