Descriptor: "Maleates blood" / Topic: acetone - Searchworks@Jio Institute Digital Library Search Results

Author: Zolodz MD, Jia M, Liu H, Henderson GN, and Stacpoole PW
Subjects: Acetone blood, Acetone metabolism, Acetone urine, Animals, Blotting, Western, Dichloroacetic Acid blood, Dichloroacetic Acid urine, Heptanoates blood, Heptanoates urine, Humans, Liver metabolism, Male, Maleates blood, Maleates urine, Rats, Sensitivity and Specificity, Tyrosine antagonists & inhibitors, Tyrosine blood, Tyrosine urine, Acetone analogs & derivatives, Dichloroacetic Acid metabolism, Gas Chromatography-Mass Spectrometry methods, Heptanoates metabolism, Maleates metabolism, Tyrosine metabolism
Abstract: We developed a sensitive method to quantitate the tyrosine metabolites maleylacetone (MA) and succinylacetone (SA) and the tyrosine metabolism inhibitor dichloroacetate (DCA) in biological specimens. Accumulation of these metabolites may be responsible for the toxicity observed when exposed to DCA. Detection limits of previous methods are 200 ng/mL (1.2 pmol/microL) (MA) and 2.6 microg/mL (16.5 pmol/microL) (SA) but the metabolites are likely present in lower levels in biological specimens. To increase sensitivity, analytes were extracted from liver, urine, plasma and cultured nerve cells before and after dosing with DCA, derivatized to their pentafluorobenzyl esters, and analyzed via GC-MS/MS.
Published: 2006
Full Text: View/download PDF

Searchworks