1. N-acetyl cysteine pre-treatment attenuates inflammatory changes in the warm ischemic murine lung.
- Author
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Geudens N, Van De Wauwer C, Neyrinck AP, Timmermans L, Vanhooren HM, Vanaudenaerde BM, Verleden GM, Verbeken E, Lerut T, and Van Raemdonck DE
- Subjects
- Animals, Biopsy, Bronchoalveolar Lavage, Cell Count, Chemokines metabolism, Cytokines metabolism, Disease Models, Animal, Female, Intercellular Signaling Peptides and Proteins metabolism, Leukocytes pathology, Lung metabolism, Lung Transplantation methods, Lymphocytes pathology, Macrophages pathology, Mice, Reperfusion Injury etiology, Reperfusion Injury metabolism, Acetylcysteine therapeutic use, Free Radical Scavengers therapeutic use, Lung pathology, Lung Transplantation pathology, Reperfusion Injury prevention & control, Warm Ischemia adverse effects
- Abstract
Background: The warm ischemic period in non-heart-beating donor lungs may contribute to a higher degree of ischemia-reperfusion injury after lung transplantation. We investigated the impact and timing of administration of N-acetyl cysteine (NAC) on inflammatory parameters., Methods: Ischemia (I) was induced by clamping the hilum of the left lung for 90 minutes, and some protocols were followed by reperfusion (R) for 4 hours. Mice were divided into nine groups (n = 6/group): three control groups ([sham] (thoracotomy only), [I] and [I+R]); two groups with saline instillation only ([saline+I] and [saline+I+R]); and four experimental groups with NAC (50 mg/kg), administered by instillation ([NAC+I], [NAC+I+R] and [I+NAC+R]) or by aerosol ([NACaero+I+R]). Cell counts and protein levels in bronchoalveolar lavage (BAL) were determined., Results: NAC administered prior to hilar clamping led to a significant decrease in macrophages and lymphocytes and interleukin (IL)-1 beta levels after ischemia. NAC also resulted in significantly fewer macrophages, lymphocytes and neutrophils as well as IL-1 beta, keratinocyte cytokine (KC), monocyte chemoattractant protein (MCP)-1 and IL-6 levels in BAL taken after reperfusion., Conclusions: NAC treatment prior to warm ischemia attenuates inflammatory changes after both the ischemic and reperfusion periods.
- Published
- 2007
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