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Abundant marine biomass in coastal regions has continued to attract increasing attention in recent times as a possible source of renewable energy. This study aimed to evaluate the effects of hydrolytic pre-treatment for the purpose of enhancing biogas yield of Laminaria digitata and Ascophyllum nodosum species found on the west coast of Scotland. Results show that L. digitata , in its natural and untreated form, appears to be more readily hydrolysable than A. nodosum . Two treatments were assessed: acid only and acid followed by enzyme. Both treatments enhanced the hydrolysis of both seaweed species, with acid-enzyme treatment providing a better performance. [ABSTRACT FROM AUTHOR]