1. Mutation of CarO participates in drug resistance in imipenem-resistant Acinetobacter baumannii.
- Author
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Zhu LJ, Chen XY, and Hou PF
- Subjects
- Acinetobacter Infections drug therapy, Acinetobacter Infections genetics, Acinetobacter baumannii genetics, Acinetobacter baumannii isolation & purification, Humans, Microbial Sensitivity Tests, Acinetobacter Infections microbiology, Acinetobacter baumannii drug effects, Anti-Bacterial Agents pharmacology, Bacterial Outer Membrane Proteins genetics, Drug Resistance, Bacterial genetics, Imipenem pharmacology, Mutation, Porins genetics
- Abstract
Objective: Acinetobacter baumannii has become an important problem because of the high drug resistance rate. The aim of this study was to assess the antimicrobial resistance profile and explore the role of membrane porin in imipenem resistance of A baumannii., Methods: A total of 63 isolates of imipenem-resistant A baumannii (IRAB) and 21 of imipenem-sensitive A baumannii (ISAB) were collected. Susceptibility testing to 16 kinds of antimicrobial agents was conducted by K-B method. PCR technique was used to detect carO and oprD genes, and sequencing was performed to compare the sequence between IRAB and ISAB. Three-dimensional structure model of CarO protein was established., Results: While ISAB isolates presented sensitive to most classes of antibiotics, isolates of IRAB displayed much higher resistance rate except tigecycline (3.2%), cefoperazone/sulbactam (28.6%), and minocycline (30.2%). All 84 isolates were observed carrying both carO and oprD genes. Further sequencing revealed important mutations of carO gene existed in IRAB in comparison with ISAB. Meanwhile, significant differences in three-dimensional structure of carO protein molecule were also found between IRAB and ISAB., Conclusions: The drug resistance profile of IRAB is increasingly severe in clinical settings. Mutation of CarO was identified as one of the molecular mechanisms involved in imipenem resistance in A baumannii., (© 2019 The Authors. Journal of Clinical Laboratory Analysis Published by Wiley Periodicals, Inc.)
- Published
- 2019
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