1. Effect of dihydrotestosterone on the upregulation of inflammatory cytokines in cultured sebocytes.
- Author
-
Lee WJ, Jung HD, Chi SG, Kim BS, Lee SJ, Kim DW, Kim MK, and Kim JC
- Subjects
- Acne Vulgaris immunology, Acne Vulgaris pathology, Cell Culture Techniques, Cells, Cultured, Dihydrotestosterone pharmacology, Fluorescent Antibody Technique, Humans, Interleukin-6 genetics, Interleukin-6 immunology, Keratinocytes drug effects, Keratinocytes immunology, Keratinocytes pathology, Organ Culture Techniques, Sebaceous Glands pathology, Sebum metabolism, Tumor Necrosis Factor-alpha genetics, Tumor Necrosis Factor-alpha immunology, Up-Regulation, Acne Vulgaris metabolism, Inflammation Mediators metabolism, Interleukin-6 metabolism, Keratinocytes metabolism, Tumor Necrosis Factor-alpha metabolism
- Abstract
Acne is a complex, chronic and common skin disorder of pilosebaceous units. Hyperkeratinization of keratinocytes, increased sebum excretion from sebocytes via androgen stimulation and inflammatory cytokines are the major factors involved in the pathophysiology of acne. In addition, it is known that keratinocytes play an important role in acne synthesizing a number of inflammatory cytokines. However, the nature of the association between inflammatory cytokines and sebocytes in acne remains unclear. Culture of sebocytes provides a new insight into the participation of dihydrotestosterone (DHT) in the production of inflammatory cytokines in acne. To examine the possible involvement of DHT in the production of inflammatory cytokines in the cultured sebocytes, we used immunohistochemistry and RT-PCR to compare the expression of interleukin-1 (IL-1), IL-6, tumor necrosis factor-alpha (TNF-alpha). Upregulation of IL-6 and TNF-alpha in immunohistochemistry, and increase in RNA amplification for IL-6 and TNF-alpha were observed after addition of DHT compared with the control. This study suggests that DHT may not only be involved in sebum production but also in production of proinflammatory cytokines in acne.
- Published
- 2010
- Full Text
- View/download PDF