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The centrally staged combustor is an effective way to reduce NOx emissions from combustors. However, combustion instability caused by the mutual coupling between flames and acoustics during the combustion process is almost unavoidable. To better understand this problem, the effect of the swirl rotational direction is investigated in this paper using two different schemes with co-swirl and counter-swirl configurations. Pressure fluctuations and flame dynamics are investigated under self-excited combustion oscillation conditions. The CH* chemiluminescence distribution captured by a high-speed camera is utilized to characterize the flame macrostructure and heat release fluctuations. Furthermore, non-oscillating reaction velocity fields are acquired using particle image velocimetry (PIV) technology. The results indicate that the amplitude and frequency of the counter-swirl scheme are higher than those of the co-swirl scheme at varying main stage equivalence ratios. Combining the results from dynamic mode decomposition and the local Rayleigh index, it is found that the heat release regions of the counter-swirl scheme are mainly concentrated in the shear layer. Higher velocity gradients, vorticities, and strain rates in the inner shear layer (ISL) and outer shear layer (OSL) for the counter-swirl scheme are verified using PIV technology. The driving sources of thermoacoustic oscillations are located in the regions of the ISL, OSL, and the area where the flame impinges on the sidewall of the liner. Additionally, the counter-swirl scheme exhibits larger vorticities and strain rates in the ISL and OSL, facilitating the development of thermoacoustic oscillations. [ABSTRACT FROM AUTHOR]