1. Use of synthetic peptides for the detection of antibodies against the nef regulating protein in sera of HIV-infected patients.
- Author
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Sabatier JM, Clerget-Raslain B, Fontan G, Fenouillet E, Rochat H, Granier C, Gluckman JC, Van Rietschoten J, Montagnier L, and Bahraoui E
- Subjects
- Amino Acid Sequence, Female, GTP-Binding Proteins genetics, GTP-Binding Proteins immunology, Gene Products, nef, Genes, Regulator, HIV genetics, HIV physiology, Humans, Male, Molecular Sequence Data, Recombinant Proteins genetics, Recombinant Proteins immunology, Retroviridae Proteins genetics, Virus Replication, nef Gene Products, Human Immunodeficiency Virus, Acquired Immunodeficiency Syndrome immunology, HIV immunology, HIV Antibodies analysis, Peptides chemical synthesis, Retroviridae Proteins immunology
- Abstract
Human sera were tested for the presence of anti-nef antibodies by radioimmunoassay (RIA), with recombinant radiolabelled nef expressed in E. coli. Of the 300 HIV-positive sera tested by RIA, 70 +/- 5.3% were found to be anti-nef positive. Anti-nef antibodies bound to nef with a high affinity (K 0.5 = 2.2 x 10(-9) M). In 31 of the sera, the specificity of anti-nef antibodies was further analysed by enzyme-linked immunosorbent assay (ELISA) with large synthetic peptides ranging from 31 to 66 amino acid residues and spanning the total sequence of nef from HIV-1. The results obtained showed that the immunodominant antigenic sites of nef were located close to the N- and C-terminal regions of the molecule.
- Published
- 1989
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