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Your search keyword '"DNA, Protozoan cerebrospinal fluid"' showing total 4 results
Start Over Descriptor "DNA, Protozoan cerebrospinal fluid" Topic acquired immunodeficiency syndrome
4 results on '"DNA, Protozoan cerebrospinal fluid"'

1. Molecular identification of Trypanosoma cruzi I tropism for central nervous system in Chagas reactivation due to AIDS.

Author

Burgos JM, Begher S, Silva HM, Bisio M, Duffy T, Levin MJ, Macedo AM, and Schijman AG

Subjects
Adult, Animals, Bolivia, Central Nervous System parasitology, Central Nervous System Protozoal Infections diagnosis, Central Nervous System Protozoal Infections etiology, Chagas Disease parasitology, DNA, Protozoan blood, DNA, Protozoan cerebrospinal fluid, Electron Transport Complex IV genetics, Fatal Outcome, Humans, Male, Microsatellite Repeats genetics, Polymorphism, Genetic, Polymorphism, Restriction Fragment Length, Recurrence, Tropism genetics, Trypanosoma cruzi genetics, Trypanosoma cruzi isolation & purification, AIDS-Related Opportunistic Infections parasitology, Acquired Immunodeficiency Syndrome complications, Central Nervous System Protozoal Infections parasitology, Chagas Disease etiology, Trypanosoma cruzi physiology
Abstract

Trypanosoma cruzi lineages, microsatellite allelic polymorphism, and mithocondrial gene haplotypes were directly typified from peripheral blood and cerebrospinal fluid specimens of a Bolivian patient with Chagas disease with accompanying AIDS and central nervous system severe involvement. Of note, the patient's blood was infected by a mixture of T. cruzi I and T. cruzi IId/e polyclonal populations while the cerebrospinal fluid showed only a monoclonal T. cruzi I population. Our findings do not corroborate the original assumption of innocuity for T. cruzi I in the southern cone of the Americas and highlight lineage I tropism for central nervous system causing lethal Chagas reactivation.

Published
2008

2. Detection of Toxoplasma gondii, Epstein-Barr virus, and JC virus DNAs in the cerebrospinal fluid in acquired immunodeficiency syndrome patients with focal central nervous system complications.

Author

Tachikawa N, Goto M, Hoshino Y, Gatanaga H, Yasuoka A, Wakabayashi T, Katano H, Kimura S, Oka S, and Iwamoto A

Subjects
Animals, Base Sequence, DNA Primers genetics, DNA, Protozoan genetics, DNA, Viral genetics, Female, Herpesvirus 4, Human genetics, Herpesvirus 4, Human isolation & purification, Humans, JC Virus genetics, JC Virus isolation & purification, Leukoencephalopathy, Progressive Multifocal complications, Leukoencephalopathy, Progressive Multifocal diagnosis, Lymphoma, AIDS-Related diagnosis, Male, Polymerase Chain Reaction methods, Toxoplasma genetics, Toxoplasma isolation & purification, Toxoplasmosis, Cerebral complications, Toxoplasmosis, Cerebral diagnosis, Acquired Immunodeficiency Syndrome complications, Brain Diseases complications, Brain Diseases diagnosis, DNA, Protozoan cerebrospinal fluid, DNA, Viral cerebrospinal fluid
Abstract

Object: Toxoplasmic encephalitis (TE), primary central nervous system lymphoma (PCNSL) and progressive multifocal leukoencephalopathy (PML) are major central nervous system (CNS) diseases in patients with acquired immunodeficiency syndrome (AIDS). We assessed the diagnostic value of polymerase chain reaction (PCR) in the detection of DNAs of Toxoplasma gondii (T. gondii), Epstein-Barr virus (EBV) and JC virus (JCV) in the cerebrospinal fluid (CSF)., Methods: We compared the PCR results with those of pathological findings at autopsy., Patients or Materials: The present study included 23 autopsies representing those in whom CSF samples were obtained before death while the patient was hospitalized or at autopsy., Results: The threshold levels for PCR detection were 4 tachyzoites of T. gondii, 5-15 genomes of EBV and 10 genomes of JCV. We identified T. gondii DNA in 4 out of 5 autopsy-defined cases of TE, EBV DNA in 5 out of 5 cases with PCNSL, and JCV DNA in 2 out of 2 cases with PML. The specificity of PCR was 100% in TE, 78% in PCNSL, and 100% in PML., Conclusion: Although the number of cases was relatively small in this study, PCR correctly identified T. gondii DNA in those cases in which PML or PCNSL was the sole clinical diagnosis. Our results indicate that PCR examination of CSF is a clinically useful tool for the diagnosis of focal brain lesions in patients with AIDS.

Published
1999
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3. Focal brain lesions in acquired immunodeficiency syndrome: DNA diagnosis and further monitoring.

Author

Kira J

Subjects
Animals, Brain Diseases genetics, DNA, Protozoan cerebrospinal fluid, DNA, Protozoan genetics, DNA, Viral cerebrospinal fluid, DNA, Viral genetics, Herpesvirus 4, Human genetics, Humans, JC Virus genetics, Leukoencephalopathy, Progressive Multifocal complications, Leukoencephalopathy, Progressive Multifocal diagnosis, Leukoencephalopathy, Progressive Multifocal genetics, Lymphoma, AIDS-Related complications, Lymphoma, AIDS-Related diagnosis, Lymphoma, AIDS-Related genetics, Polymerase Chain Reaction, Toxoplasma genetics, Toxoplasmosis, Cerebral complications, Toxoplasmosis, Cerebral diagnosis, Toxoplasmosis, Cerebral genetics, Acquired Immunodeficiency Syndrome complications, Brain Diseases complications, Brain Diseases diagnosis
Published
1999
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4. Detection of Toxoplasma gondii DNA by polymerase chain reaction in cerebrospinal fluid from AIDS patients with cerebral toxoplasmosis.

Author

Lebech M, Lebech AM, Nelsing S, Vuust J, Mathiesen L, and Petersen E

Subjects
Animals, Base Sequence, DNA, Protozoan chemistry, Humans, Molecular Sequence Data, Polymerase Chain Reaction, Toxoplasma genetics, Toxoplasmosis, Cerebral complications, Acquired Immunodeficiency Syndrome complications, DNA, Protozoan cerebrospinal fluid, Toxoplasma isolation & purification, Toxoplasmosis, Cerebral diagnosis
Published
1992
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