1. Effect of Acupotomy on FAK-PI3K Signaling Pathways in KOA Rabbit Articular Cartilages.
- Author
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Ma, Shi-Ning, Xie, Zhan-guo, Guo, Yan, Yu, Jia-Ni, Lu, Juan, Zhang, Wei, Wang, Li-Juan, Xu, Jing, Zhao, Rui-Li, Zhou, Shuai, and Guo, Chang-Qing
- Subjects
ACUPUNCTURE ,ANIMAL experimentation ,ARTICULAR cartilage ,BIOLOGICAL models ,CARTILAGE cells ,CELLULAR signal transduction ,COMPARATIVE studies ,ELECTROACUPUNCTURE ,GENE expression ,POLYMERASE chain reaction ,PROTEINS ,RABBITS ,STATISTICAL sampling ,WESTERN immunoblotting ,TREATMENT effectiveness ,REVERSE transcriptase polymerase chain reaction ,DESCRIPTIVE statistics - Abstract
Objective. By observing the needle-knife of KOA rabbit morphology, knee joint cartilage p-FAK, p-PI3K, Aggrecan gene, and protein expression, to study the effect of needle-knife to promote cartilage cell synthesis metabolism mechanism. Method. 49 male New Zealand rabbits, randomly divided into normal group (Z), model group (M), model-inhibitors (MP), needle-knife group (D), needle-knife inhibitors group (DP), electroacupuncture group (E), and electroacupuncture inhibitors (EP). RT-PCR and Western Blot were used to test each animal cartilage p-FAK, p-PI3K, and Aggrecan gene and protein expression level. Results. Compared with N group, p-FAK and p-PI3K protein and mRNA expression of M group, D group, and E group increased (P < 0.05), while the protein and mRNA expression of Aggrecan reduced (P < 0.05). Compared with M group, p-FAK, p-PI3K, Aggrecan protein, and mRNA of E and D group increased (P < 0.05). Compared with E group, p-FAK, p-PI3K, Aggrecan protein, and mRNA expression of D group increased (P < 0.05); after adding inhibitors, p-FAK, p-PI3K, Aggrecan protein, and mRNA expression reduced (P < 0.05). Conclusion. Needle-knife therapy can promote the repairment of cartilage cells by activating FAK-PI3K signaling pathways, promoting the synthesis of cartilage cell metabolism. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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