1. The effect of miR-146a on STAT1 expression and apoptosis in acute lymphoblastic leukemia Jurkat cells
- Author
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Hua Zheng, Tong Chen, Chunling Han, Weihong Yan, Hua Guo, and Feng Suo
- Subjects
0301 basic medicine ,Agonist ,Cancer Research ,Oncogene ,medicine.diagnostic_test ,medicine.drug_class ,Cell ,apoptosis ,Transfection ,Articles ,acute lymphoblastic leukemia ,Biology ,Jurkat cells ,In vitro ,Flow cytometry ,miR-146a ,03 medical and health sciences ,030104 developmental biology ,medicine.anatomical_structure ,STAT1 ,Oncology ,Apoptosis ,medicine ,Cancer research - Abstract
The effect of miR-146a-dependent regulation of STAT1 on apoptosis in acute lymphoblastic leukemia (ALL) Jurkat cells was investigated. The miR-146a mimic and miR-146a inhibitor vectors were constructed in vitro, and experimental grouping was as follows: Control group (untreated Jurkat cells), empty vector group (Jurkat cells transfected with empty vector), agonist group (Jurkat cells transfected with miR-146a mimic) and the inhibitor group (Jurkat cells transfected with miR-146a inhibitor). Western blot analysis was used to observe the expression, respectively, of STAT1, p-STAT1 and Bcl-xL, and flow cytometry was used to test apoptosis in Jurkat cells. STAT1 and p-STAT1 expression in the agonist group was higher than that in the control and empty vector groups, but lower in the inhibitor group, and differences were statistically significant (P
- Published
- 2016