1. Intravenous high mobility group box 1 upregulates the expression of HIF-1α in the myocardium via a protein kinase B-dependent pathway in rats following acute myocardial ischemia
- Author
-
Qian‑Feng Han, Min Zhou, Yan-Hong Zhou, Lei Wu, Tao Liu, Heng‑Chen Yao, Ke‑Li Tian, Lan‑Hua Wang, Mei Zhang, and De‑Yong Zhang
- Subjects
0301 basic medicine ,Cancer Research ,high mobility group box 1 ,030204 cardiovascular system & hematology ,Biochemistry ,Phosphatidylinositol 3-Kinases ,0302 clinical medicine ,Troponin I ,hypoxia inducible factor-1α ,Myocardial infarction ,HMGB1 Protein ,Phosphoinositide-3 Kinase Inhibitors ,ischemia reperfusion injury ,biology ,Kinase ,Articles ,Oncogene Protein v-akt ,Oncology ,Molecular Medicine ,medicine.symptom ,acute myocardial ischemia ,Signal Transduction ,medicine.medical_specialty ,Morpholines ,Ischemia ,Myocardial Reperfusion Injury ,chemical and pharmacologic phenomena ,HMGB1 ,03 medical and health sciences ,Internal medicine ,Genetics ,medicine ,Animals ,Humans ,Molecular Biology ,PI3K/AKT/mTOR pathway ,Tumor Necrosis Factor-alpha ,business.industry ,Akt/PKB signaling pathway ,Myocardium ,Hypoxia (medical) ,Hypoxia-Inducible Factor 1, alpha Subunit ,medicine.disease ,Rats ,030104 developmental biology ,Endocrinology ,Gene Expression Regulation ,Chromones ,Immunology ,biology.protein ,business ,Proto-Oncogene Proteins c-akt - Abstract
The effects of intravenous high mobility group box 1 (HMGB1) on myocardial ischemia/reperfusion (I/R) injury remains to be elucidated. The purpose of the present study was to investigate the effects of intravenous HMGB1 on the expression of hypoxia inducible factor-1α (HIF-1α) in the myocardium of rats following acute myocardial ischemia, and to examine the effects of intravenous HMGB1 on myocardial I/R injury. Male Wistar rats were divided into the following groups: Sham operation group (n=10), a group exposed to ischemia for 30 min and reperfusion for 4 h (I/R group) as a control (n=10), an HMGB group, in which 100 ng/kg HMGB was administered intravenously 30 min prior to ischemia (n=10), an LY group, in which LY294002, an inhibitor of phosphoinositide 3-kinase (PI3K), was administered intravenously (0.3 mg/kg) 40 min prior to ischemia (n=10), and the HMGB1+LY group, in which HMGB1 (100 ng/kg) and LY294002 (0.3 mg/kg) were administered intravenously 30 min and 40 min prior to ischemia, respectively (n=10). The serum levels of cardiac troponin I (cTnI) and tumor necrosis factor-α (TNF-α), and myocardial infarct size were measured. The expression levels of phosphorylated Akt and HIF-1α were investigated using western blot analyses. The results showed that pre-treatment with HMGB1 significantly decreased serum levels of cTnI, and TNF-α, and reduced myocardial infarct size following 4 h reperfusion (all P
- Published
- 2015