1. ADAM17 promotes lymph node metastasis in gastric cancer via activation of the Notch and Wnt signaling pathways
- Author
-
Lei Wang, Yang Zhang, Xuan Sun, Daguang Wang, Wei Li, and Jian Suo
- Subjects
Male ,0301 basic medicine ,Metastasis ,0302 clinical medicine ,disintegrin and metalloproteinase domain-containing protein 17 ,Cell Movement ,Odds Ratio ,prognostic factor ,Lymph node ,Receptors, Notch ,lymph node metastasis ,Wnt signaling pathway ,Articles ,General Medicine ,Middle Aged ,Prognosis ,Immunohistochemistry ,Gene Expression Regulation, Neoplastic ,medicine.anatomical_structure ,Area Under Curve ,Lymphatic Metastasis ,030220 oncology & carcinogenesis ,Female ,ADAM9 ,Signal Transduction ,Adult ,signaling pathway ,Cell Survival ,ADAM17 Protein ,Biology ,03 medical and health sciences ,Stomach Neoplasms ,Cell Line, Tumor ,Genetics ,medicine ,Humans ,Viability assay ,Aged ,Neoplasm Staging ,Oncogene ,gastric cancer ,Membrane Proteins ,Cancer ,medicine.disease ,Wnt Proteins ,ADAM Proteins ,030104 developmental biology ,Cancer cell ,Cancer research ,Lymph Nodes ,Biomarkers - Abstract
Disintegrin and metalloproteinase domain-containing proteins (ADAMs) have been implicated in cell adhesion, signaling and migration. The aim of the present study was to identify key members of the ADAM protein family associated with the metastasis of gastric cancer and to evaluate their clinical significance. A total of 193 patients with gastric cancer and positive lymph node metastasis were enrolled. Key members of the ADAM family associated with lymph node metastasis were identified. The correlations between survival times and the clinicopathological features of patients were investigated. Furthermore, ADAM17 expression in gastric cancer cells with different metastatic potentials was determined. ADAM17 was overexpressed in BGC-823 cells and suppressed in SGC-7901 cells to further investigate its effects on cell viability and migration. The key pathways associated with ADAM17 were identified by gene set enrichment analysis (GSEA). It was found that ADAM9 and ADAM17 were significantly upregulated in gastric cancer and positive metastatic lymph node tissues. Further, there was a strong correlation between the survival times of patients and ADAM17 expression. ADAM17 was upregulated in gastric cancer cells with high metastatic potential. The viability of BGC-823 cells significantly increased following ADAM17 overexpression, whereas the viability and migration of SGC-7901 cells decreased following ADAM17 suppression. GSEA and western blot analysis revealed a positive correlation between the Notch and Wnt signaling pathways with ADAM17 expression. In conclusion, the increased expression of ADAM17 promoted the progression of gastric cancer, potentially via Notch and/or Wnt signaling pathway activation, and ADAM17 may serve as a useful prognostic marker.
- Published
- 2018