1. Prognostic significance of microRNA-141 expression and its tumor suppressor function in human pancreatic ductal adenocarcinoma.
- Author
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Zhu ZM, Xu YF, Su QJ, Du JD, Tan XL, Tu YL, Tan JW, and Jiao HB
- Subjects
- Adenocarcinoma mortality, Adenocarcinoma pathology, Apoptosis genetics, Carcinoma, Pancreatic Ductal mortality, Carcinoma, Pancreatic Ductal pathology, Caspase 3, Cell Line, Tumor, Cell Proliferation, Down-Regulation genetics, Female, Gene Expression Regulation, Neoplastic, Genes, Tumor Suppressor, Humans, Lymphatic Metastasis genetics, Lymphatic Metastasis pathology, Male, MicroRNAs biosynthesis, Middle Aged, Pancreas pathology, Pancreatic Neoplasms mortality, Pancreatic Neoplasms pathology, Prognosis, RNA Interference, RNA, Small Interfering, Transcription Factors, YAP-Signaling Proteins, Adaptor Proteins, Signal Transducing genetics, Adenocarcinoma genetics, Carcinoma, Pancreatic Ductal genetics, MicroRNAs genetics, Pancreatic Neoplasms genetics, Phosphoproteins genetics
- Abstract
Increasing evidence shows that dysregulation of microRNAs is correlated with tumor development. This study was performed to determine the expression of miR-141 and investigate its clinical significance in pancreatic ductal adenocarcinoma (PDAC). Taqman quantitative RT-PCR was used to detect miR-141 expressions in 94 PDAC tissues and 16 nontumorous pancreatic tissues. Correlations between miR-141 expression and clinicopathologic features and prognosis of patients were statistically analyzed. The effects of miR-141 expression on growth and apoptosis of PDAC cell line (PANC-1) were determined by MTT, colony formation, and flow cytometry assays. Potential target genes were identified by luciferase reporter and Western blot assays. The expression level of miR-141 in PDAC tissues was significantly lower than that in corresponding nontumorous tissues. Downregulation of miR-141 correlated with poorer pT and pN status, advanced clinical stage, and lymphatic invasion. Also, low miR-141 expression in PDAC tissues was significantly correlated with shorter overall survival, and multivariate analysis showed that miR-141 was an independent prognostic factor for PDAC patients. Further, functional researches suggested that miR-141 inhibits growth and colony formation, and enhances caspase-3-dependent apoptosis in PANC-1 cells by targeting Yes-associated protein-1 (YAP1). Therefore, miR-141 is an independent prognostic factor for PDAC patients, and functions as a tumor suppressor gene by targeting YAP1.
- Published
- 2014
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