1. High levels of ANXA2 are characteristic of malignant salivary gland tumors.
- Author
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Cardoso CM, de Jesus SF, de Souza MG, Queiroz LDRP, Santos EM, Dos Santos EP, Oliveira LP, Cordeiro Santos CK, Santos SHS, de Paula AMB, Farias LC, and Guimaraes ALS
- Subjects
- Adenoma, Pleomorphic pathology, Biomarkers, Tumor metabolism, Carcinoma, Adenoid Cystic pathology, Carcinoma, Mucoepidermoid pathology, Case-Control Studies, Humans, Proteome, Proteomics, Salivary Gland Neoplasms pathology, Adenoma, Pleomorphic metabolism, Annexin A2 metabolism, Carcinoma, Adenoid Cystic metabolism, Carcinoma, Mucoepidermoid metabolism, Salivary Gland Neoplasms metabolism
- Abstract
Objective: Malignant salivary gland tumors (MSGTs) present different phenotypic characteristics and various clinical outcomes, which proved to be a diagnostic challenge. Considering the heterogeneity of MSGT, this study aims to identify molecule related to the nature of MSGT., Methods: For screening, proteomic analysis comparing MSGT with pleomorphic adenoma (PA) and salivary gland was performed. The MSGT-associated protein which presented in the higher number in the Gene Expression Omnibus (GEO) database was selected. To validate the data, immunohistochemistry (IHC) was performed in 14 patients with PA, 22 patients with MSGT, and 14 controls., Results: 16 proteins were associated with MSGT. ANXA2 was the primary protein, according to GEO database analyses. ANXA2 was most expressed in the cell membrane. However, some ANXA2 staining was also observed in the cytoplasm and nucleus. ANXA2 was highly expressed in MSGT in comparison with control. Also, ANXA2 has a higher expression in adenocarcinoma not otherwise specified (ANOS) and myoepithelial carcinoma (MC) in comparison with PA., Conclusion: In conclusion, this study demonstrated that MSGT presented higher levels of ANXA2 in comparison with normal salivary glands. Also, ANXA2 might be interesting as a molecular marker of ANOS and MS., (© 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2019
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