1. The use of fluorescence lifetime technology in benign and malignant thyroid tissues.
- Author
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Nakache G, Yahav G, Siloni GH, Barshack I, Alon E, Wolf M, and Fixler D
- Subjects
- Adenoma metabolism, Female, Fluorescein-5-isothiocyanate pharmacology, Fluorescent Antibody Technique, Direct, Goiter, Nodular metabolism, Humans, Male, Microscopy, Fluorescence, Thyroglobulin antagonists & inhibitors, Thyroid Gland pathology, Adenoma diagnostic imaging, Goiter, Nodular diagnostic imaging, Thyroid Gland diagnostic imaging, Thyroid Neoplasms diagnostic imaging
- Abstract
Objective: To explore the use of fluorescence lifetime imaging microscopy in thyroid tissues, and to investigate how different thyroid lesions affect fluorescence lifetime., Method: Fluorescence lifetime measurements were taken of fresh frozen thyroid surgical specimens stained with fluorescein isothiocyanate tagged anti-thyroglobulin monoclonal antibodies., Results: The mean fluorescence lifetime measurements in 12 patients - 3 with multinodular goitre, 4 with follicular adenoma, 4 with papillary thyroid carcinoma and 1 with follicular carcinoma - were 3.16 ns (range, 2.66-3.52 ns), 3.75 ns (range, 2.99-4.57 ns), 2.97 ns (range, 2.57-3.21 ns) and 3.61 ns, respectively. The fluorescence lifetime of follicular adenoma patients was higher than that of papillary thyroid carcinoma patients by 26 per cent (p = 0.058). The fluorescence lifetime in the follicular carcinoma patient was similar to the follicular adenoma group, but higher than in the papillary thyroid carcinoma group by 22 per cent (p = 0.01)., Conclusion: Fluorescence lifetime measurements varied in different thyroid pathologies, possibly because of tissue-scale structural influences.
- Published
- 2019
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