1. Glucose-free conditions induce the expression of AMPK in dental pulp cells.
- Author
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Muramatsu, Takashi, Yuasa, Kazuhiro, Ebihara, Kumi, Shibukawa, Yoshiyuki, Ohta, Kazumasa, Furusawa, Masahiro, and Shimono, Masaki
- Subjects
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PROTEIN kinases , *ADENOSINE monophosphate , *DENTAL pulp , *POLYMERASE chain reaction , *IMMUNOFLUORESCENCE , *WESTERN immunoblotting , *GLUCOSE in the body - Abstract
Abstract: Objectives: This study is aimed to test whether glucose-free conditions induce the activation of adenosine monophosphate-activated protein kinase (AMPK) and, to investigate association with AMPK expression and cell viability in human dental pulp cells. Design: Human dental pulp cells were initially maintained in culture medium containing glucose and the medium was subsequently changed to glucose-free medium. To evaluate the expression of AMPK, quantitative real-time RT-PCR, Western blot analysis and immunofluorescence were carried out. Cell viability was evaluated by MTT assay. Results: The expression of AMPK mRNA in glucose free conditions was 2.0–2.5 fold higher than the control at 1, 2 and 3h (P <0.01). The expression of phosphorylated-AMPK was characterized by Western blot analysis and by immunofluorescence. Compound C-pre-treated group showed a decline of both AMPK expression and cell viability, while AICAR-pre-treated group showed an increase of AMPK and maintain of cell viability at regular level. Conclusions: AMPK plays an important role on fluctuating in accordance with glucose availability and protects cell viability from glucose free condition in human dental pulp cells. [Copyright &y& Elsevier]
- Published
- 2013
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