1. Improvement of human pancreatic islet quality after co-culture with human adipose-derived stem cells.
- Author
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de Souza BM, Rodrigues M, de Oliveira FS, da Silva LPA, Bouças AP, Portinho CP, Dos Santos BP, Camassola M, Rocha D, Lysakowski S, Martini J, Leitão CB, Nardi NB, Bauer AC, and Crispim D
- Subjects
- Apoptosis drug effects, Apoptosis genetics, Chemokines metabolism, Coculture Techniques, Culture Media, Endoplasmic Reticulum Stress drug effects, Endoplasmic Reticulum Stress genetics, Gene Expression Regulation drug effects, Humans, Inflammation genetics, Inflammation Mediators metabolism, Insulin pharmacology, Intercellular Signaling Peptides and Proteins metabolism, Neovascularization, Physiologic drug effects, Neovascularization, Physiologic genetics, Stem Cells drug effects, Time Factors, Tissue Survival drug effects, Adipose Tissue cytology, Islets of Langerhans cytology, Stem Cells cytology
- Abstract
The aim of this study was to investigate whether co-culture of human islets with adipose-derived stem cells (ASCs) can improve islet quality and to evaluate which factors play a role in the protective effect of ASCs against islet dysfunction. Islets and ASCs were cultured in three experimental groups for 24 h, 48 h, and 72 h: 1) indirect co-culture of islets with ASC monolayer (Islets/ASCs); 2) islets alone; and 3) ASCs alone. Co-culture with ASCs improved islet viability and function in all culture time-points analyzed. VEGFA, HGF, IL6, IL8, IL10, CCL2, IL1B, and TNF protein levels were increased in supernatants of islet/ASC group compared to islets alone, mainly after 24 h. Moreover, VEGFA, IL6, CCL2, HIF1A, XIAP, CHOP, and NFKBIA genes were differentially expressed in islets from the co-culture condition compared to islets alone. In conclusion, co-culture of islets with ASCs promotes improvements in islet quality., Competing Interests: Declaration of competing interest The authors declare no competing interests., (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Published
- 2020
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