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Start Over Author "Sihu Wang" Topic adipose tissue
3 results on '"Sihu Wang"'

1. Transcriptome profiling reveals differential expression of genes potentially involved in muscle and adipose tissue development of cattle

Author

Sihu Wang, Sayed Haidar Abbas Raza, Chugang Mei, Kai Zhu, Matthew Garcia, Nicola M. Schreurs, Chengcheng Liang, Xinran Yang, and Linsen Zan

Subjects
Adipose tissue, Bioinformatic analyses, Bovine adipocytes, Cattle, Developmental regulation, Expressed genes, Biotechnology, TP248.13-248.65, Biology (General), QH301-705.5
Abstract

Background: To identify differentially expressed genes (DEGs) between muscle and adipose in cattle, we analyzed the data from the RNA sequencing of three Angus×Qinchuan crossbred cattle. Results: Searched the Gene Expression Omnibus (GEO) for a microarray dataset of Yan yellow cattle, GSE49992. After the DEGs were identified, we used STRING and Cytoscape to construct a protein–protein interaction (PPI) network, subsequently analyzing the major modules of key genes. In total, 340 DEGs were discovered, including 21 hub genes, which were mainly enriched in muscle contraction, skeletal muscle contraction, troponin complex, lipid particle, Z disc, tropomyosin binding, and actin filament binding. Conclusions: In summary, these genes can be regarded as candidate biomarkers for the regulation of muscle and adipose development.How to cite: Wang S, Raza SHA, Mei C, et al. Transcriptome profiling reveals differential expression of genes potentially involved in muscle and adipose tissue development of cattle. Electron J Biotechnol 2020;48. https://doi.org/10.1016/j.ejbt.2020.09.004.

Published
2020
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2. Transcriptome profiling reveals differential expression of genes potentially involved in muscle and adipose tissue development of cattle

Author

Matthew D. Garcia, Sayed Haidar Abbas Raza, Chengcheng Liang, Linsen Zan, Chugang Mei, Sihu Wang, Kai Zhu, Nicola M. Schreurs, and Xinran Yang

Subjects
0106 biological sciences, 0301 basic medicine, Microarray, lcsh:Biotechnology, Adipose tissue, RNA-Seq, Computational biology, Biology, 01 natural sciences, Applied Microbiology and Biotechnology, Expressed genes, Transcriptome, 03 medical and health sciences, Tropomyosin binding, 010608 biotechnology, lcsh:TP248.13-248.65, Bioinformatic analyses, Gene, lcsh:QH301-705.5, Developmental regulation, Bovine adipocytes, 030104 developmental biology, lcsh:Biology (General), Actin filament binding, Cattle, Lipid particle, Biotechnology
Abstract

Background: To identify differentially expressed genes (DEGs) between muscle and adipose in cattle, we analyzed the data from the RNA sequencing of three Angus × Qinchuan crossbred cattle. Results: Searched the Gene Expression Omnibus (GEO) for a microarray dataset of Yan yellow cattle, GSE49992. After the DEGs were identified, we used STRING and Cytoscape to construct a protein–protein interaction (PPI) network, subsequently analyzing the major modules of key genes. In total, 340 DEGs were discovered, including 21 hub genes, which were mainly enriched in muscle contraction, skeletal muscle contraction, troponin complex, lipid particle, Z disc, tropomyosin binding, and actin filament binding. Conclusions: In summary, these genes can be regarded as candidate biomarkers for the regulation of muscle and adipose development. Normal 0 false false false EN-US X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabla normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:8.0pt; mso-para-margin-left:0cm; line-height:107%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri",sans-serif; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;}

Published
2020

3. The role of BAMBI in regulating adipogenesis and myogenesis and the association between its polymorphisms and growth traits in cattle

Author

Jingchun Sun, Linsen Zan, Xinran Yang, Weiyi Zhang, Chugang Mei, Yue Ning, and Sihu Wang

Subjects
0301 basic medicine, Muscle Fibers, Skeletal, Mutation, Missense, Muscle Proteins, Adipose tissue, Biology, Muscle Development, Bone morphogenetic protein, MyoD, Polymorphism, Single Nucleotide, Myoblasts, Andrology, Random Allocation, 03 medical and health sciences, 0302 clinical medicine, Gene Frequency, Consensus Sequence, Adipocytes, Genetics, Animals, Amino Acid Sequence, RNA, Messenger, Muscle, Skeletal, Molecular Biology, Cells, Cultured, Adipogenesis, Sequence Homology, Amino Acid, Myogenesis, Gene Expression Profiling, Wnt signaling pathway, Lipid Droplets, General Medicine, 030104 developmental biology, Animals, Newborn, Haplotypes, Gene Knockdown Techniques, 030220 oncology & carcinogenesis, MYF6, Cattle, Female, BAMBI, Sequence Alignment, Selective Breeding, Signal Transduction
Abstract

Bone morphogenic protein and activin membrane-bound inhibitor (BAMBI) is a transmembrane protein that affects the growth, development and muscle regeneration of the body by regulating the TGF-β, BMP and Wnt signaling pathways. Studies have found that BAMBI has important regulatory functions in skeletal muscle and preadipocytes in vivo and in vitro. However, research on this protein in cattle is lacking. In this study, to determine the role of BAMBI in the growth and development of cattle, we first found that the expression of BAMBI in adipose tissue and longissimus muscle of newborn and adult Qinchuan beef cattle was significantly different. Then we showed that BAMBI knockdown promoted the differentiation of bovine preadipocytes and suppressed myoblast myogenesis, as indicated by the increased lipid droplets and the decreased myotubes, as well as the corresponding significant changes in the expression of PPARγ, C/EBPα, C/EBPβ, FABP4, MyoD, MyoG and Myf6. Finally, to further verify the effect of BAMBI on the growth performance of cattle, we identified seven novel SNPs in the BAMBI genomic region, which were significantly correlated with one or more growth traits (p

Published
2020
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