1. Lipophilic quaternary ammonium salt acts as a mucosal adjuvant when co-administered by the nasal route with vaccine antigens.
- Author
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Klinguer C, Beck A, De-Lys P, Bussat MC, Blaecke A, Derouet F, Bonnefoy JY, Nguyen TN, Corvaïa N, and Velin D
- Subjects
- Administration, Intranasal, Animals, Diphtheria Toxoid administration & dosage, Female, Immunity, Mucosal, Mice, Mice, Inbred BALB C, Sigmodontinae, T-Lymphocytes immunology, Tetanus Toxoid administration & dosage, Adjuvants, Immunologic administration & dosage, Quaternary Ammonium Compounds administration & dosage, Respiratory Syncytial Viruses immunology, Vaccines, Synthetic administration & dosage
- Abstract
Nasal administration of vaccines is an attractive approach which offers several significant advantages over traditional intramuscular vaccine delivery. These advantages include easier administration and induction of immune responses in the mucosal secretions of the body. In this study we describe a new potent nasal adjuvant, dimethyldioctadecylammonium bromide (DDA), that induces both mucosal and systemic immune responses when co-administered with diphtheria toxoid (DT), tetanus toxoid (TT) and BBG2Na antigens. In particular, we show that the nasal delivery of recombinant fragment (BBG2Na) of the G protein of respiratory syncytial virus (RSV) mixed with DDA induces both local and systemic anti-RSV immune responses and protects against viral challenge. Furthermore, we provide evidence that the DDA+BBG2Na vaccine does not induce lung immunopathology upon subsequent RSV challenge.
- Published
- 2001
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